TY  - JOUR
AU  - Tasic, Ljubica
AU  - Avramović, Nataša
AU  - Jadranin, Milka
AU  - Quintero, Melissa
AU  - Stanisic, Danijela
AU  - Martins, Lucas G.
AU  - Costa, Tássia Brena Barroso Carneiro
AU  - Novak, Estela
AU  - Odone, Vicente
AU  - Rivas, Maria
AU  - Aguiar, Talita
AU  - Carraro, Dirce Maria
AU  - Werneck da Cunha, Isabela
AU  - Lima da Costa, Cecilia Maria
AU  - Maschietto, Mariana
AU  - Krepischi, Ana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5568
AB  - Cancer is one of the leading causes of death in children and adolescents worldwide; among the types of liver cancer, hepatoblastoma (HBL) is the most common in childhood. Although it affects only two to three individuals in a million, it is mostly asymptomatic at diagnosis, so by the time it is detected it has already advanced. There are specific recommendations regarding HBL treatment, and ongoing studies to stratify the risks of HBL, understand the pathology, and predict prognostics and survival rates. Although magnetic resonance imaging spectroscopy is frequently used in diagnostics of HBL, high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy of HBL tissues is scarce. Using this technique, we studied the alterations among tissue metabolites of ex vivo samples from (a) HBL and non-cancer liver tissues (NCL), (b) HBL and adjacent non-tumor samples, and (c) two regions of the same HBL samples, one more centralized and the other at the edge of the tumor. It was possible to identify metabolites in HBL, then metabolites from the HBL center and the border samples, and link them to altered metabolisms in tumor tissues, highlighting their potential as biochemical markers. Metabolites closely related to liver metabolisms such as some phospholipids, triacylglycerides, fatty acids, glucose, and amino acids showed differences between the tissues.
PB  - MDPI AG
T2  - Biomedicines
T1  - High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks
VL  - 10
IS  - 12
SP  - 3091
DO  - 10.3390/biomedicines10123091
ER  - 

@article{
author = "Tasic, Ljubica and Avramović, Nataša and Jadranin, Milka and Quintero, Melissa and Stanisic, Danijela and Martins, Lucas G. and Costa, Tássia Brena Barroso Carneiro and Novak, Estela and Odone, Vicente and Rivas, Maria and Aguiar, Talita and Carraro, Dirce Maria and Werneck da Cunha, Isabela and Lima da Costa, Cecilia Maria and Maschietto, Mariana and Krepischi, Ana",
year = "2022",
abstract = "Cancer is one of the leading causes of death in children and adolescents worldwide; among the types of liver cancer, hepatoblastoma (HBL) is the most common in childhood. Although it affects only two to three individuals in a million, it is mostly asymptomatic at diagnosis, so by the time it is detected it has already advanced. There are specific recommendations regarding HBL treatment, and ongoing studies to stratify the risks of HBL, understand the pathology, and predict prognostics and survival rates. Although magnetic resonance imaging spectroscopy is frequently used in diagnostics of HBL, high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy of HBL tissues is scarce. Using this technique, we studied the alterations among tissue metabolites of ex vivo samples from (a) HBL and non-cancer liver tissues (NCL), (b) HBL and adjacent non-tumor samples, and (c) two regions of the same HBL samples, one more centralized and the other at the edge of the tumor. It was possible to identify metabolites in HBL, then metabolites from the HBL center and the border samples, and link them to altered metabolisms in tumor tissues, highlighting their potential as biochemical markers. Metabolites closely related to liver metabolisms such as some phospholipids, triacylglycerides, fatty acids, glucose, and amino acids showed differences between the tissues.",
publisher = "MDPI AG",
journal = "Biomedicines",
title = "High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks",
volume = "10",
number = "12",
pages = "3091",
doi = "10.3390/biomedicines10123091"
}

Tasic, L., Avramović, N., Jadranin, M., Quintero, M., Stanisic, D., Martins, L. G., Costa, T. B. B. C., Novak, E., Odone, V., Rivas, M., Aguiar, T., Carraro, D. M., Werneck da Cunha, I., Lima da Costa, C. M., Maschietto, M.,& Krepischi, A.. (2022). High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks. in Biomedicines
MDPI AG., 10(12), 3091.
https://doi.org/10.3390/biomedicines10123091

Tasic L, Avramović N, Jadranin M, Quintero M, Stanisic D, Martins LG, Costa TBBC, Novak E, Odone V, Rivas M, Aguiar T, Carraro DM, Werneck da Cunha I, Lima da Costa CM, Maschietto M, Krepischi A. High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks. in Biomedicines. 2022;10(12):3091.
doi:10.3390/biomedicines10123091 .

Tasic, Ljubica, Avramović, Nataša, Jadranin, Milka, Quintero, Melissa, Stanisic, Danijela, Martins, Lucas G., Costa, Tássia Brena Barroso Carneiro, Novak, Estela, Odone, Vicente, Rivas, Maria, Aguiar, Talita, Carraro, Dirce Maria, Werneck da Cunha, Isabela, Lima da Costa, Cecilia Maria, Maschietto, Mariana, Krepischi, Ana, "High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks" in Biomedicines, 10, no. 12 (2022):3091,
https://doi.org/10.3390/biomedicines10123091 . .

TY  - JOUR
AU  - Tasić, Ljubica
AU  - Avramović, Nataša
AU  - Quintero, Melissa
AU  - Stanišić, Danijela
AU  - Martins, Lucas
AU  - Brena da Costa, Tássia
AU  - Jadranin, Milka
AU  - Accioly de Souza, Maria Theresa
AU  - Faria, Paulo
AU  - de Camargo, Beatriz
AU  - de Sá Pereira, Bruna Maria
AU  - Maschietto, Mariana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5323
AB  - Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.
PB  - MDPI
T2  - Diagnostic
T1  - A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy
VL  - 12
IS  - 1
SP  - 2
EP  - 10
DO  - 10.3390/diagnostics12010157
ER  - 

@article{
author = "Tasić, Ljubica and Avramović, Nataša and Quintero, Melissa and Stanišić, Danijela and Martins, Lucas and Brena da Costa, Tássia and Jadranin, Milka and Accioly de Souza, Maria Theresa and Faria, Paulo and de Camargo, Beatriz and de Sá Pereira, Bruna Maria and Maschietto, Mariana",
year = "2022",
abstract = "Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.",
publisher = "MDPI",
journal = "Diagnostic",
title = "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy",
volume = "12",
number = "1",
pages = "2-10",
doi = "10.3390/diagnostics12010157"
}

Tasić, L., Avramović, N., Quintero, M., Stanišić, D., Martins, L., Brena da Costa, T., Jadranin, M., Accioly de Souza, M. T., Faria, P., de Camargo, B., de Sá Pereira, B. M.,& Maschietto, M.. (2022). A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic
MDPI., 12(1), 2-10.
https://doi.org/10.3390/diagnostics12010157

Tasić L, Avramović N, Quintero M, Stanišić D, Martins L, Brena da Costa T, Jadranin M, Accioly de Souza MT, Faria P, de Camargo B, de Sá Pereira BM, Maschietto M. A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic. 2022;12(1):2-10.
doi:10.3390/diagnostics12010157 .

Tasić, Ljubica, Avramović, Nataša, Quintero, Melissa, Stanišić, Danijela, Martins, Lucas, Brena da Costa, Tássia, Jadranin, Milka, Accioly de Souza, Maria Theresa, Faria, Paulo, de Camargo, Beatriz, de Sá Pereira, Bruna Maria, Maschietto, Mariana, "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy" in Diagnostic, 12, no. 1 (2022):2-10,
https://doi.org/10.3390/diagnostics12010157 . .