TY  - JOUR
AU  - Nicoleti, Jorge Luis
AU  - Braga, Erik Sobrinho
AU  - Stanisic, Danijela
AU  - Jadranin, Milka
AU  - Façanha, Débora Andréa Evangelista
AU  - Barral, Thiago Doria
AU  - Hanna, Samira Abdallah
AU  - Azevedo, Vasco
AU  - Meyer, Roberto
AU  - Tasic, Ljubica
AU  - Portela, Ricardo Wagner
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7094
AB  - Caseous lymphadenitis (CLA), an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants, is highly prevalent worldwide. Economic losses have already been associated with the disease, and little is known about the host–pathogen relationship associated with the disease. The present study aimed to perform a metabolomic study of the C. pseudotuberculosis infection in goats. Serum samples were collected from a herd of 173 goats. The animals were classified as controls (not infected), asymptomatic (seropositives but without detectable CLA clinical signs), and symptomatic (seropositive animals presenting CLA lesions), according to microbiological isolation and immunodiagnosis. The serum samples were analyzed using nuclear magnetic resonance (1H-NMR), nuclear Overhauser effect spectroscopy (NOESY), and Carr–Purcell–Meiboom–Gill (CPMG) sequences. The NMR data were analyzed using chemometrics, and principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were performed to discover specific biomarkers responsible for discrimination between the groups. A high dissemination of the infection by C. pseudotuberculosis was observed, being 74.57% asymptomatic and 11.56% symptomatic. In the evaluation of 62 serum samples by NMR, the techniques were satisfactory in the discrimination of the groups, being also complementary and mutually confirming, demonstrating possible biomarkers for the infection by the bacterium. Twenty metabolites of interest were identified by NOESY and 29 by CPMG, such as tryptophan, polyunsaturated fatty acids, formic acid, NAD+, and 3-hydroxybutyrate, opening promising possibilities for the use of these results in new therapeutic, immunodiagnosis, and immunoprophylactic tools, as well as for studies of the immune response against C. pseudotuberculosis.
PB  - Springer Science and Business Media LLC
T2  - Applied Microbiology and Biotechnology
T1  - A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats
VL  - 107
IS  - 14
SP  - 4593
EP  - 4603
DO  - 10.1007/s00253-023-12595-0
ER  - 

@article{
author = "Nicoleti, Jorge Luis and Braga, Erik Sobrinho and Stanisic, Danijela and Jadranin, Milka and Façanha, Débora Andréa Evangelista and Barral, Thiago Doria and Hanna, Samira Abdallah and Azevedo, Vasco and Meyer, Roberto and Tasic, Ljubica and Portela, Ricardo Wagner",
year = "2023",
abstract = "Caseous lymphadenitis (CLA), an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants, is highly prevalent worldwide. Economic losses have already been associated with the disease, and little is known about the host–pathogen relationship associated with the disease. The present study aimed to perform a metabolomic study of the C. pseudotuberculosis infection in goats. Serum samples were collected from a herd of 173 goats. The animals were classified as controls (not infected), asymptomatic (seropositives but without detectable CLA clinical signs), and symptomatic (seropositive animals presenting CLA lesions), according to microbiological isolation and immunodiagnosis. The serum samples were analyzed using nuclear magnetic resonance (1H-NMR), nuclear Overhauser effect spectroscopy (NOESY), and Carr–Purcell–Meiboom–Gill (CPMG) sequences. The NMR data were analyzed using chemometrics, and principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were performed to discover specific biomarkers responsible for discrimination between the groups. A high dissemination of the infection by C. pseudotuberculosis was observed, being 74.57% asymptomatic and 11.56% symptomatic. In the evaluation of 62 serum samples by NMR, the techniques were satisfactory in the discrimination of the groups, being also complementary and mutually confirming, demonstrating possible biomarkers for the infection by the bacterium. Twenty metabolites of interest were identified by NOESY and 29 by CPMG, such as tryptophan, polyunsaturated fatty acids, formic acid, NAD+, and 3-hydroxybutyrate, opening promising possibilities for the use of these results in new therapeutic, immunodiagnosis, and immunoprophylactic tools, as well as for studies of the immune response against C. pseudotuberculosis.",
publisher = "Springer Science and Business Media LLC",
journal = "Applied Microbiology and Biotechnology",
title = "A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats",
volume = "107",
number = "14",
pages = "4593-4603",
doi = "10.1007/s00253-023-12595-0"
}

Nicoleti, J. L., Braga, E. S., Stanisic, D., Jadranin, M., Façanha, D. A. E., Barral, T. D., Hanna, S. A., Azevedo, V., Meyer, R., Tasic, L.,& Portela, R. W.. (2023). A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats. in Applied Microbiology and Biotechnology
Springer Science and Business Media LLC., 107(14), 4593-4603.
https://doi.org/10.1007/s00253-023-12595-0

Nicoleti JL, Braga ES, Stanisic D, Jadranin M, Façanha DAE, Barral TD, Hanna SA, Azevedo V, Meyer R, Tasic L, Portela RW. A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats. in Applied Microbiology and Biotechnology. 2023;107(14):4593-4603.
doi:10.1007/s00253-023-12595-0 .

Nicoleti, Jorge Luis, Braga, Erik Sobrinho, Stanisic, Danijela, Jadranin, Milka, Façanha, Débora Andréa Evangelista, Barral, Thiago Doria, Hanna, Samira Abdallah, Azevedo, Vasco, Meyer, Roberto, Tasic, Ljubica, Portela, Ricardo Wagner, "A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats" in Applied Microbiology and Biotechnology, 107, no. 14 (2023):4593-4603,
https://doi.org/10.1007/s00253-023-12595-0 . .

TY  - CONF
AU  - Tasić, Ljubica
AU  - Martins, Lucas
AU  - Braga, Erik Sobrinho
AU  - Novais, M.
AU  - Jadranin, Milka
AU  - Stanišić, Danijela
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7277
AB  - The infection with SARS-CoV-2 mostly leads to mild disease, but some individuals progress to severe disease with death outcome [1]. These severe cases are driven by the host response to the infection, with multisystem dysfunctions [1,2]. Timely intervention in patients in critical conditions could be crucial for their survival. Therefore, it is of paramount importance to understand the disease phase and its progression markers. Serum samples from two cohorts of patients, with mild (n = 92) and severe disease (n = 41) were analyzed, with two subgroups in each cohort, positive and negative to the virus. The samples were age and gender-matched to healthy volunteers (n = 87), too. Three NMR data sets (1H-NMR, T2, and edited by diffusion) were recorded on a 600 MHz Bruker AVANCE III spectrometer, and analyzed in MetaboAnalyst 5.0. Serum metabolomics showed that proteins, very low and low-density lipoproteins (VLDL and LDL), phospholipids, 
lysophosphatidylcholines among other 50 metabolites were disrupted. Furthermore, changes in the levels of NAD(P)+, hyperglycemia and dysregulated glucose were detrimental in people with COVID-19. Glu cose excess, increased levels of VLDL, and decreased proteins were related to the disease severity, and might be important for determining a patient’s phase of the disease, and NMR can greatly add to the differentiation between severe and mild symptoms.
PB  - NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)
C3  - 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event
T1  - Impressions on COVID-19 by NMR
SP  - OC24
UR  - https://hdl.handle.net/21.15107/rcub_cer_7277
ER  - 

@conference{
author = "Tasić, Ljubica and Martins, Lucas and Braga, Erik Sobrinho and Novais, M. and Jadranin, Milka and Stanišić, Danijela",
year = "2021",
abstract = "The infection with SARS-CoV-2 mostly leads to mild disease, but some individuals progress to severe disease with death outcome [1]. These severe cases are driven by the host response to the infection, with multisystem dysfunctions [1,2]. Timely intervention in patients in critical conditions could be crucial for their survival. Therefore, it is of paramount importance to understand the disease phase and its progression markers. Serum samples from two cohorts of patients, with mild (n = 92) and severe disease (n = 41) were analyzed, with two subgroups in each cohort, positive and negative to the virus. The samples were age and gender-matched to healthy volunteers (n = 87), too. Three NMR data sets (1H-NMR, T2, and edited by diffusion) were recorded on a 600 MHz Bruker AVANCE III spectrometer, and analyzed in MetaboAnalyst 5.0. Serum metabolomics showed that proteins, very low and low-density lipoproteins (VLDL and LDL), phospholipids, 
lysophosphatidylcholines among other 50 metabolites were disrupted. Furthermore, changes in the levels of NAD(P)+, hyperglycemia and dysregulated glucose were detrimental in people with COVID-19. Glu cose excess, increased levels of VLDL, and decreased proteins were related to the disease severity, and might be important for determining a patient’s phase of the disease, and NMR can greatly add to the differentiation between severe and mild symptoms.",
publisher = "NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)",
journal = "10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event",
title = "Impressions on COVID-19 by NMR",
pages = "OC24",
url = "https://hdl.handle.net/21.15107/rcub_cer_7277"
}

Tasić, L., Martins, L., Braga, E. S., Novais, M., Jadranin, M.,& Stanišić, D.. (2021). Impressions on COVID-19 by NMR. in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event
NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)., OC24.
https://hdl.handle.net/21.15107/rcub_cer_7277

Tasić L, Martins L, Braga ES, Novais M, Jadranin M, Stanišić D. Impressions on COVID-19 by NMR. in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event. 2021;:OC24.
https://hdl.handle.net/21.15107/rcub_cer_7277 .

Tasić, Ljubica, Martins, Lucas, Braga, Erik Sobrinho, Novais, M., Jadranin, Milka, Stanišić, Danijela, "Impressions on COVID-19 by NMR" in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event (2021):OC24,
https://hdl.handle.net/21.15107/rcub_cer_7277 .