TY  - JOUR
AU  - Ivanović, Ljubica
AU  - Topalović, Ana
AU  - Bogdanović, Višnja
AU  - Djurović, Dijana
AU  - Mugoša, Boban
AU  - Jadranin, Milka
AU  - Tešević, Vele
AU  - Beškoski, Vladimir
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4797
AB  - Purpose: The purpose of this paper is to determine the polyphenol content, antioxidative potential and antiproliferative activity of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. Design/methodology/approach: Swiss chard was grown in the open field (Lješkopolje, Montenegro) where it was subjected to different fertilization and irrigation regimes. Chard samples were analyzed for previously identified polyphenols and for antioxidant parameters. Additionally, in order to complete the biological activities, chard extracts were tested for antiproliferative activity against MFC-7 and HT-29 tumor cell lines. Findings: The polyphenols identified in Swiss chard were flavonoids: vitexin-2?-O-xyloside, vitexin-6?-O-acetyl-2?-O-xyloside, vitexin-6?-O-malonyl-2?-O-xyloside and isorhamnetin-3,7-diglucoside. In the antioxidant tests, DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), chard extract had values of 7.00 and 8.50 (mean values) µmol Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equ/d.w., respectively. The tested chard extracts inhibited cell proliferation at different concentrations (3.125–50.0 µg/mL) against the MCF-7 cell line, after 24 and 48 h of incubation. The antiproliferative activity, expressed in terms of IC50, was 32.97 and 86.45 µg/mL after 24 h of incubation and 20.76 and 23.33 µg/mL after 48 h of incubation, for treated and untreated chard extracts, respectively. Originality/value: These data suggest Montenegrin Swiss chard grown under different irrigation and fertilization treatments can be considered as a functional food and should be included in an everyday diet. The collected data could help in the growth improvement of chard with functional food properties.
PB  - Emerald Publishing Limited
T2  - British Food Journal
T1  - Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes
VL  - 123
IS  - 7
SP  - 2335
EP  - 2348
DO  - 10.1108/BFJ-11-2020-1062
ER  - 

@article{
author = "Ivanović, Ljubica and Topalović, Ana and Bogdanović, Višnja and Djurović, Dijana and Mugoša, Boban and Jadranin, Milka and Tešević, Vele and Beškoski, Vladimir",
year = "2021",
abstract = "Purpose: The purpose of this paper is to determine the polyphenol content, antioxidative potential and antiproliferative activity of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. Design/methodology/approach: Swiss chard was grown in the open field (Lješkopolje, Montenegro) where it was subjected to different fertilization and irrigation regimes. Chard samples were analyzed for previously identified polyphenols and for antioxidant parameters. Additionally, in order to complete the biological activities, chard extracts were tested for antiproliferative activity against MFC-7 and HT-29 tumor cell lines. Findings: The polyphenols identified in Swiss chard were flavonoids: vitexin-2?-O-xyloside, vitexin-6?-O-acetyl-2?-O-xyloside, vitexin-6?-O-malonyl-2?-O-xyloside and isorhamnetin-3,7-diglucoside. In the antioxidant tests, DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), chard extract had values of 7.00 and 8.50 (mean values) µmol Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equ/d.w., respectively. The tested chard extracts inhibited cell proliferation at different concentrations (3.125–50.0 µg/mL) against the MCF-7 cell line, after 24 and 48 h of incubation. The antiproliferative activity, expressed in terms of IC50, was 32.97 and 86.45 µg/mL after 24 h of incubation and 20.76 and 23.33 µg/mL after 48 h of incubation, for treated and untreated chard extracts, respectively. Originality/value: These data suggest Montenegrin Swiss chard grown under different irrigation and fertilization treatments can be considered as a functional food and should be included in an everyday diet. The collected data could help in the growth improvement of chard with functional food properties.",
publisher = "Emerald Publishing Limited",
journal = "British Food Journal",
title = "Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes",
volume = "123",
number = "7",
pages = "2335-2348",
doi = "10.1108/BFJ-11-2020-1062"
}

Ivanović, L., Topalović, A., Bogdanović, V., Djurović, D., Mugoša, B., Jadranin, M., Tešević, V.,& Beškoski, V.. (2021). Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. in British Food Journal
Emerald Publishing Limited., 123(7), 2335-2348.
https://doi.org/10.1108/BFJ-11-2020-1062

Ivanović L, Topalović A, Bogdanović V, Djurović D, Mugoša B, Jadranin M, Tešević V, Beškoski V. Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. in British Food Journal. 2021;123(7):2335-2348.
doi:10.1108/BFJ-11-2020-1062 .

Ivanović, Ljubica, Topalović, Ana, Bogdanović, Višnja, Djurović, Dijana, Mugoša, Boban, Jadranin, Milka, Tešević, Vele, Beškoski, Vladimir, "Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes" in British Food Journal, 123, no. 7 (2021):2335-2348,
https://doi.org/10.1108/BFJ-11-2020-1062 . .

TY  - JOUR
AU  - Spasić, Snežana
AU  - Martinov, Olga B.
AU  - Bogdanović, Višnja
AU  - Miletić, Srđan
AU  - Vrvić, Miroslav
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/325
AB  - We examined effect of α-tocopherol addition and influence of nitric oxide from sodium nitroprusside (SNP) on free thiol content and SOD activity in exponentially growing K562 cells (human erythroleukaemia) in RPMI 1640 (Sigma) supplemented with 2 mM glutamine, 10 % heat-inactivated fetal calf serum and antibiotics at 37 oC in a humidified 5 % carbon dioxide atmosphere. Cells concentration was of 0.85 x 106 per flask. Our results indicate potential dual role of α-tocopherol: induction of more reduced cell state (increased free -SH) and increased resistance of cancer cells to treatment which induce oxidative stress (increased SOD). α-Tocopherol pretreated K562 cells do not expected respond on the SNP treatment. .
AB  - Ispitivali smo efekat dodatka α-tokoferola i uticaj azot-oksida iz natrijum-nitroprusida (NNP) na sadržaj slobodnih tiol grupa i SOD aktivnost kod K562 ćelija (humana eritroleukemija) u eksponencijalnoj fazi rasta, koje su gajene u RPMI 1640 (Sigma) sa dodatkom 2 mM glutamina, 10 % toplotom-inaktiviranog fetalnog govedjeg seruma i antibiotika, na 37 oC u vlažnoj atmosferi sa 5 % ugljen-dioksida. Koncentracija ćelija bila je 0,85 x 106 po posudi. Naši rezultati ukazuju na moguću dvostruku ulogu α-tokoferola kod ovih ćelija: indukciju više redukovanog stanja (porast slobodnih -SH grupa), ali i porast njihove otpornosti na tretmane koji indukuju oksidacioni stres (porast SOD aktivnosti). α-Tokoferolom pretretirane K562 ćelije ne daju očekivani odgovor na tretman sa NNP. .
T2  - Zbornik radova Tehnološkog fakulteta, Leskovac
T1  - Effect of α-tocopherol on free thiol content and SOD activity in K562 cells
T1  - Efekat α-tokoferola na količinu slobodnih tiola i SOD aktivnost kod K562 ćelija
IS  - 16
SP  - 51
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_cer_325
ER  - 

@article{
author = "Spasić, Snežana and Martinov, Olga B. and Bogdanović, Višnja and Miletić, Srđan and Vrvić, Miroslav",
year = "2007",
abstract = "We examined effect of α-tocopherol addition and influence of nitric oxide from sodium nitroprusside (SNP) on free thiol content and SOD activity in exponentially growing K562 cells (human erythroleukaemia) in RPMI 1640 (Sigma) supplemented with 2 mM glutamine, 10 % heat-inactivated fetal calf serum and antibiotics at 37 oC in a humidified 5 % carbon dioxide atmosphere. Cells concentration was of 0.85 x 106 per flask. Our results indicate potential dual role of α-tocopherol: induction of more reduced cell state (increased free -SH) and increased resistance of cancer cells to treatment which induce oxidative stress (increased SOD). α-Tocopherol pretreated K562 cells do not expected respond on the SNP treatment. ., Ispitivali smo efekat dodatka α-tokoferola i uticaj azot-oksida iz natrijum-nitroprusida (NNP) na sadržaj slobodnih tiol grupa i SOD aktivnost kod K562 ćelija (humana eritroleukemija) u eksponencijalnoj fazi rasta, koje su gajene u RPMI 1640 (Sigma) sa dodatkom 2 mM glutamina, 10 % toplotom-inaktiviranog fetalnog govedjeg seruma i antibiotika, na 37 oC u vlažnoj atmosferi sa 5 % ugljen-dioksida. Koncentracija ćelija bila je 0,85 x 106 po posudi. Naši rezultati ukazuju na moguću dvostruku ulogu α-tokoferola kod ovih ćelija: indukciju više redukovanog stanja (porast slobodnih -SH grupa), ali i porast njihove otpornosti na tretmane koji indukuju oksidacioni stres (porast SOD aktivnosti). α-Tokoferolom pretretirane K562 ćelije ne daju očekivani odgovor na tretman sa NNP. .",
journal = "Zbornik radova Tehnološkog fakulteta, Leskovac",
title = "Effect of α-tocopherol on free thiol content and SOD activity in K562 cells, Efekat α-tokoferola na količinu slobodnih tiola i SOD aktivnost kod K562 ćelija",
number = "16",
pages = "51-56",
url = "https://hdl.handle.net/21.15107/rcub_cer_325"
}

Spasić, S., Martinov, O. B., Bogdanović, V., Miletić, S.,& Vrvić, M.. (2007). Effect of α-tocopherol on free thiol content and SOD activity in K562 cells. in Zbornik radova Tehnološkog fakulteta, Leskovac(16), 51-56.
https://hdl.handle.net/21.15107/rcub_cer_325

Spasić S, Martinov OB, Bogdanović V, Miletić S, Vrvić M. Effect of α-tocopherol on free thiol content and SOD activity in K562 cells. in Zbornik radova Tehnološkog fakulteta, Leskovac. 2007;(16):51-56.
https://hdl.handle.net/21.15107/rcub_cer_325 .

Spasić, Snežana, Martinov, Olga B., Bogdanović, Višnja, Miletić, Srđan, Vrvić, Miroslav, "Effect of α-tocopherol on free thiol content and SOD activity in K562 cells" in Zbornik radova Tehnološkog fakulteta, Leskovac, no. 16 (2007):51-56,
https://hdl.handle.net/21.15107/rcub_cer_325 .

TY  - CONF
AU  - Spasić, Snežana
AU  - Martinov, Olga B.
AU  - Bogdanović, Višnja
AU  - Miletić, Srđan
AU  - Vrvić, Miroslav
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5098
PB  - Balkan society for microbiology
PB  - Montenegrin society for microbiology
C3  - Programme, Abstract book - 5th Balkan congress for microbiology, Budva, Montenegro, October 2007
T1  - Effect of α-tocopherol and α-tocopherol + nitric oxide donor on L929 cells
SP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_cer_5098
ER  - 

@conference{
author = "Spasić, Snežana and Martinov, Olga B. and Bogdanović, Višnja and Miletić, Srđan and Vrvić, Miroslav",
year = "2007",
publisher = "Balkan society for microbiology, Montenegrin society for microbiology",
journal = "Programme, Abstract book - 5th Balkan congress for microbiology, Budva, Montenegro, October 2007",
title = "Effect of α-tocopherol and α-tocopherol + nitric oxide donor on L929 cells",
pages = "146",
url = "https://hdl.handle.net/21.15107/rcub_cer_5098"
}

Spasić, S., Martinov, O. B., Bogdanović, V., Miletić, S.,& Vrvić, M.. (2007). Effect of α-tocopherol and α-tocopherol + nitric oxide donor on L929 cells. in Programme, Abstract book - 5th Balkan congress for microbiology, Budva, Montenegro, October 2007
Balkan society for microbiology., 146.
https://hdl.handle.net/21.15107/rcub_cer_5098

Spasić S, Martinov OB, Bogdanović V, Miletić S, Vrvić M. Effect of α-tocopherol and α-tocopherol + nitric oxide donor on L929 cells. in Programme, Abstract book - 5th Balkan congress for microbiology, Budva, Montenegro, October 2007. 2007;:146.
https://hdl.handle.net/21.15107/rcub_cer_5098 .

Spasić, Snežana, Martinov, Olga B., Bogdanović, Višnja, Miletić, Srđan, Vrvić, Miroslav, "Effect of α-tocopherol and α-tocopherol + nitric oxide donor on L929 cells" in Programme, Abstract book - 5th Balkan congress for microbiology, Budva, Montenegro, October 2007 (2007):146,
https://hdl.handle.net/21.15107/rcub_cer_5098 .