TY  - JOUR
AU  - Nikolić, Milan R.
AU  - Stanić-Vučinić, Dragana
AU  - Baričević, Ivona I.
AU  - Jones, David R.B.
AU  - Nedić, Olgica
AU  - Niketić, Vesna
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4247
AB  - Objective: The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. Design and methods: We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). Results: When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and correlated with the loss of cholesterol from Hb-Ch. Conclusion: The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo.
PB  - Elsevier
T2  - Clinical Biochemistry
T1  - Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma
VL  - 40
IS  - 5-6
SP  - 305
EP  - 309
DO  - 10.1016/j.clinbiochem.2006.11.005
ER  - 

@article{
author = "Nikolić, Milan R. and Stanić-Vučinić, Dragana and Baričević, Ivona I. and Jones, David R.B. and Nedić, Olgica and Niketić, Vesna",
year = "2007",
abstract = "Objective: The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. Design and methods: We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). Results: When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and correlated with the loss of cholesterol from Hb-Ch. Conclusion: The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo.",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma",
volume = "40",
number = "5-6",
pages = "305-309",
doi = "10.1016/j.clinbiochem.2006.11.005"
}

Nikolić, M. R., Stanić-Vučinić, D., Baričević, I. I., Jones, D. R.B., Nedić, O.,& Niketić, V.. (2007). Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma. in Clinical Biochemistry
Elsevier., 40(5-6), 305-309.
https://doi.org/10.1016/j.clinbiochem.2006.11.005

Nikolić MR, Stanić-Vučinić D, Baričević II, Jones DR, Nedić O, Niketić V. Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma. in Clinical Biochemistry. 2007;40(5-6):305-309.
doi:10.1016/j.clinbiochem.2006.11.005 .

Nikolić, Milan R., Stanić-Vučinić, Dragana, Baričević, Ivona I., Jones, David R.B., Nedić, Olgica, Niketić, Vesna, "Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma" in Clinical Biochemistry, 40, no. 5-6 (2007):305-309,
https://doi.org/10.1016/j.clinbiochem.2006.11.005 . .