TY  - JOUR
AU  - Grujić, Marica
AU  - Dojnov, Biljana
AU  - Potočnik, Ivana
AU  - Atanasova, Lea
AU  - Duduk, Bojan
AU  - Srebotnik, Ewald
AU  - Druzhinina, Irina S.
AU  - Kubicek, Christian P.
AU  - Vujčić, Zoran
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3382
AB  - Lignocellulosic plant biomass is the world’s most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain—UB483FTG2/ TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (β-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.
PB  - Springer Science and Business Media LLC
T2  - World Journal of Microbiology and Biotechnology
T1  - Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw
VL  - 35
IS  - 12
SP  - 194
DO  - 10.1007/s11274-019-2774-y
ER  - 

@article{
author = "Grujić, Marica and Dojnov, Biljana and Potočnik, Ivana and Atanasova, Lea and Duduk, Bojan and Srebotnik, Ewald and Druzhinina, Irina S. and Kubicek, Christian P. and Vujčić, Zoran",
year = "2019",
abstract = "Lignocellulosic plant biomass is the world’s most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain—UB483FTG2/ TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (β-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.",
publisher = "Springer Science and Business Media LLC",
journal = "World Journal of Microbiology and Biotechnology",
title = "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw",
volume = "35",
number = "12",
pages = "194",
doi = "10.1007/s11274-019-2774-y"
}

Grujić, M., Dojnov, B., Potočnik, I., Atanasova, L., Duduk, B., Srebotnik, E., Druzhinina, I. S., Kubicek, C. P.,& Vujčić, Z.. (2019). Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology and Biotechnology
Springer Science and Business Media LLC., 35(12), 194.
https://doi.org/10.1007/s11274-019-2774-y

Grujić M, Dojnov B, Potočnik I, Atanasova L, Duduk B, Srebotnik E, Druzhinina IS, Kubicek CP, Vujčić Z. Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology and Biotechnology. 2019;35(12):194.
doi:10.1007/s11274-019-2774-y .

Grujić, Marica, Dojnov, Biljana, Potočnik, Ivana, Atanasova, Lea, Duduk, Bojan, Srebotnik, Ewald, Druzhinina, Irina S., Kubicek, Christian P., Vujčić, Zoran, "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw" in World Journal of Microbiology and Biotechnology, 35, no. 12 (2019):194,
https://doi.org/10.1007/s11274-019-2774-y . .

TY  - JOUR
AU  - Dojnov, Biljana
AU  - Grujić, Marica
AU  - Vujčić, Zoran
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3408
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3144
AB  - A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.
PB  - Wiley-Blackwell, Hoboken
T2  - Electrophoresis
T1  - Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex
VL  - 36
IS  - 15
SP  - 1724
EP  - 1727
DO  - 10.1002/elps.201400541
ER  - 

@article{
author = "Dojnov, Biljana and Grujić, Marica and Vujčić, Zoran",
year = "2015",
abstract = "A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Electrophoresis",
title = "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex",
volume = "36",
number = "15",
pages = "1724-1727",
doi = "10.1002/elps.201400541"
}

Dojnov, B., Grujić, M.,& Vujčić, Z.. (2015). Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis
Wiley-Blackwell, Hoboken., 36(15), 1724-1727.
https://doi.org/10.1002/elps.201400541

Dojnov B, Grujić M, Vujčić Z. Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis. 2015;36(15):1724-1727.
doi:10.1002/elps.201400541 .

Dojnov, Biljana, Grujić, Marica, Vujčić, Zoran, "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex" in Electrophoresis, 36, no. 15 (2015):1724-1727,
https://doi.org/10.1002/elps.201400541 . .