TY  - CONF
AU  - Marinković, Vesna
AU  - Lugonja, Nikoleta
AU  - Jesic, Milos
AU  - Hajdarpasic, Vesna
AU  - Sekulovic, Gordana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7251
AB  - Background and Aims:
Human milk (HM) feeding has an important role in prevention of oxydative stress in preterm
infants. Human milk is a complex biological fluid with an antioxidative function. Antioxidative
enzymes present in HM are superoxid dismutase (SOD), glutatione peroxidase (GSH-Px) and
glutatione reductase (GR).
Evaluation of antioxidative enzymes (SOD, GSH-Px, GR) in HM after heat treatment, ie
pasteurisation of donor HM previously freezed for 7 days in HM bank, using spectrophotometric
methods.
Methods:
Cohort study enrolled 30 mothers of preterm neonates (birth before 37 weeks of gestational age)
that established lactation. Antioxidative activity of enzymes in milk samples before and after the
heat treatment was measured with spectrophotometric methods. Study protocol was approved by
Ethical Comittee of Institute for neonatology, all mothers gave their written consent prior to
participation.
Study was supported by Ministry of education, science and tehchnological development, Serbia.
Results:
SOD, GSH-Px and GR acitivity was significantly higher in fresh mature milk samples compared
to mature, frozen for seven days and then pasteurised, milk of mothers of preterm infants
(p<0,001).
Conclusions:
Process of heat treatment and/or storage of mature milk of mothers of preterm infants reduce
enzyme acitivity of HM, thereby decreasing total antioxidative capacity. This implies that it
would be optimal to feed the preterm infants with fresh fortified HM whenever possible.
PB  - Kenes Group
C3  - 10th International Conference on Nutrition and Growth, 30 March - 1 April 2023 London, UK
T1  - Impact of freezing and pasteurization on antioxidative enzymes in milk of preterm infants' mothers
VL  - EP017/#126
UR  - https://hdl.handle.net/21.15107/rcub_cer_7251
ER  - 

@conference{
author = "Marinković, Vesna and Lugonja, Nikoleta and Jesic, Milos and Hajdarpasic, Vesna and Sekulovic, Gordana",
year = "2023",
abstract = "Background and Aims:
Human milk (HM) feeding has an important role in prevention of oxydative stress in preterm
infants. Human milk is a complex biological fluid with an antioxidative function. Antioxidative
enzymes present in HM are superoxid dismutase (SOD), glutatione peroxidase (GSH-Px) and
glutatione reductase (GR).
Evaluation of antioxidative enzymes (SOD, GSH-Px, GR) in HM after heat treatment, ie
pasteurisation of donor HM previously freezed for 7 days in HM bank, using spectrophotometric
methods.
Methods:
Cohort study enrolled 30 mothers of preterm neonates (birth before 37 weeks of gestational age)
that established lactation. Antioxidative activity of enzymes in milk samples before and after the
heat treatment was measured with spectrophotometric methods. Study protocol was approved by
Ethical Comittee of Institute for neonatology, all mothers gave their written consent prior to
participation.
Study was supported by Ministry of education, science and tehchnological development, Serbia.
Results:
SOD, GSH-Px and GR acitivity was significantly higher in fresh mature milk samples compared
to mature, frozen for seven days and then pasteurised, milk of mothers of preterm infants
(p<0,001).
Conclusions:
Process of heat treatment and/or storage of mature milk of mothers of preterm infants reduce
enzyme acitivity of HM, thereby decreasing total antioxidative capacity. This implies that it
would be optimal to feed the preterm infants with fresh fortified HM whenever possible.",
publisher = "Kenes Group",
journal = "10th International Conference on Nutrition and Growth, 30 March - 1 April 2023 London, UK",
title = "Impact of freezing and pasteurization on antioxidative enzymes in milk of preterm infants' mothers",
volume = "EP017/#126",
url = "https://hdl.handle.net/21.15107/rcub_cer_7251"
}

Marinković, V., Lugonja, N., Jesic, M., Hajdarpasic, V.,& Sekulovic, G.. (2023). Impact of freezing and pasteurization on antioxidative enzymes in milk of preterm infants' mothers. in 10th International Conference on Nutrition and Growth, 30 March - 1 April 2023 London, UK
Kenes Group., EP017/#126.
https://hdl.handle.net/21.15107/rcub_cer_7251

Marinković V, Lugonja N, Jesic M, Hajdarpasic V, Sekulovic G. Impact of freezing and pasteurization on antioxidative enzymes in milk of preterm infants' mothers. in 10th International Conference on Nutrition and Growth, 30 March - 1 April 2023 London, UK. 2023;EP017/#126.
https://hdl.handle.net/21.15107/rcub_cer_7251 .

Marinković, Vesna, Lugonja, Nikoleta, Jesic, Milos, Hajdarpasic, Vesna, Sekulovic, Gordana, "Impact of freezing and pasteurization on antioxidative enzymes in milk of preterm infants' mothers" in 10th International Conference on Nutrition and Growth, 30 March - 1 April 2023 London, UK, EP017/#126 (2023),
https://hdl.handle.net/21.15107/rcub_cer_7251 .

TY  - CONF
AU  - Marinković, Vesna
AU  - Lugonja, Nikoleta
AU  - Jesic, Milos
AU  - Hajdarpasic, Vesna
AU  - Sekulovic, Gordana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7248
AB  - Objectives and Study: Human milk (HM) is a complex biological fluid with an antioxidative function. HM feeding has an important role in prevention of antioxidative stress in preterm infants. Evaluation of total antioxidative capacity (TAC) in milk of mothers f preterm infants during lactation (colostrum, transitional and mature milk). Methods: Thirty mothers of preterm infants (infants born before 37 weeks of gestational age) who established lactation were enrolled in this cohort study. TAC of colostrum, transitional and mature milk of these mothers was evaluated using the fluorimetric method of determination of antioxidative capacity of oxygen radical absorption (ORAC). Study protocol was approved by Ethical Comittee of Institute for neonatology, all mothers gave their written consent prior to participation. Study was supported by Ministry of education, science and tehchnological development, Serbia. Results: ORAC method showed that TAC was highest in colostrum, gradual decrease was registered during lactation until reaching its lowest level in mature milk. Conclusions: Significant changes in antioxidative characteristics of human milk occur during the lactation period. Colostrum has a higher TAC compared to mature milk, as shown with ORAC method. This result distinguishes colostrum as a superior sample of HM, indicating its importance in nutrition of preterm infants.
PB  - The European Society for Paediatric Gastroenterology, Hepatology and Nutrition
C3  - Pediatric Gastroenterology and Nutrition
T1  - Evaluation of total antioxidative capacity in milk of mothers of preterm infants during lactation
VL  - 76
IS  - 1
IS  - S1
DO  - 10.1097/MPG.0000000000003823
ER  - 

@conference{
author = "Marinković, Vesna and Lugonja, Nikoleta and Jesic, Milos and Hajdarpasic, Vesna and Sekulovic, Gordana",
year = "2023",
abstract = "Objectives and Study: Human milk (HM) is a complex biological fluid with an antioxidative function. HM feeding has an important role in prevention of antioxidative stress in preterm infants. Evaluation of total antioxidative capacity (TAC) in milk of mothers f preterm infants during lactation (colostrum, transitional and mature milk). Methods: Thirty mothers of preterm infants (infants born before 37 weeks of gestational age) who established lactation were enrolled in this cohort study. TAC of colostrum, transitional and mature milk of these mothers was evaluated using the fluorimetric method of determination of antioxidative capacity of oxygen radical absorption (ORAC). Study protocol was approved by Ethical Comittee of Institute for neonatology, all mothers gave their written consent prior to participation. Study was supported by Ministry of education, science and tehchnological development, Serbia. Results: ORAC method showed that TAC was highest in colostrum, gradual decrease was registered during lactation until reaching its lowest level in mature milk. Conclusions: Significant changes in antioxidative characteristics of human milk occur during the lactation period. Colostrum has a higher TAC compared to mature milk, as shown with ORAC method. This result distinguishes colostrum as a superior sample of HM, indicating its importance in nutrition of preterm infants.",
publisher = "The European Society for Paediatric Gastroenterology, Hepatology and Nutrition",
journal = "Pediatric Gastroenterology and Nutrition",
title = "Evaluation of total antioxidative capacity in milk of mothers of preterm infants during lactation",
volume = "76",
number = "1, S1",
doi = "10.1097/MPG.0000000000003823"
}

Marinković, V., Lugonja, N., Jesic, M., Hajdarpasic, V.,& Sekulovic, G.. (2023). Evaluation of total antioxidative capacity in milk of mothers of preterm infants during lactation. in Pediatric Gastroenterology and Nutrition
The European Society for Paediatric Gastroenterology, Hepatology and Nutrition., 76(1).
https://doi.org/10.1097/MPG.0000000000003823

Marinković V, Lugonja N, Jesic M, Hajdarpasic V, Sekulovic G. Evaluation of total antioxidative capacity in milk of mothers of preterm infants during lactation. in Pediatric Gastroenterology and Nutrition. 2023;76(1).
doi:10.1097/MPG.0000000000003823 .

Marinković, Vesna, Lugonja, Nikoleta, Jesic, Milos, Hajdarpasic, Vesna, Sekulovic, Gordana, "Evaluation of total antioxidative capacity in milk of mothers of preterm infants during lactation" in Pediatric Gastroenterology and Nutrition, 76, no. 1 (2023),
https://doi.org/10.1097/MPG.0000000000003823 . .

TY  - JOUR
AU  - Minić, Simeon
AU  - Jesic, Milos
AU  - Đurović, Dijana
AU  - Miletić, Srđan
AU  - Lugonja, Nikoleta
AU  - Marinković, Vesna
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Vrvić, Miroslav
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2471
AB  - AimThere is a discrepancy between the amount of transitional milk produced by mothers of preterm infants and the low capacity of premature infants to consume it. This milk can be used in milk banks, but previous studies found that there are large variations in the level of host-defence proteins in individual samples of milk from mothers of premature infants, which implies that large individual variations in antioxidative defence composition are also possible. MethodsMilk samples were collected from 20 healthy mothers of preterm infants. We determined the values for non-enzymatic antioxidative capacity parameters (oxygen radical absorbance capacity (ORAC)), static oxidation-reduction potential (ORP), activities of antioxidant defence enzymes and the amount of vitamin C in whole milk, skim and whey fractions of transitional milk. ResultsThe main low-molecular-weight antioxidant in transitional milk is vitamin C and most of it is contained in whey. ORAC is higher in whole transitional milk than in skim milk and whey, and ORP is lower in whole transitional milk than that in skim milk and whey. Antioxidative enzyme activities are similar in all individual samples of transitional milk from mothers of preterm infants. ConclusionsOur results indicate that transitional milk of mothers of preterm infants shows slow individual variations in antioxidative defence composition; therefore, it can be used in human milk banks.
PB  - Wiley, Hoboken
T2  - Journal of Paediatrics and Child Health
T1  - Redox properties of transitional milk from mothers of preterm infants
VL  - 54
IS  - 2
SP  - 160
EP  - 164
DO  - 10.1111/jpc.13676
ER  - 

@article{
author = "Minić, Simeon and Jesic, Milos and Đurović, Dijana and Miletić, Srđan and Lugonja, Nikoleta and Marinković, Vesna and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Vrvić, Miroslav",
year = "2018",
abstract = "AimThere is a discrepancy between the amount of transitional milk produced by mothers of preterm infants and the low capacity of premature infants to consume it. This milk can be used in milk banks, but previous studies found that there are large variations in the level of host-defence proteins in individual samples of milk from mothers of premature infants, which implies that large individual variations in antioxidative defence composition are also possible. MethodsMilk samples were collected from 20 healthy mothers of preterm infants. We determined the values for non-enzymatic antioxidative capacity parameters (oxygen radical absorbance capacity (ORAC)), static oxidation-reduction potential (ORP), activities of antioxidant defence enzymes and the amount of vitamin C in whole milk, skim and whey fractions of transitional milk. ResultsThe main low-molecular-weight antioxidant in transitional milk is vitamin C and most of it is contained in whey. ORAC is higher in whole transitional milk than in skim milk and whey, and ORP is lower in whole transitional milk than that in skim milk and whey. Antioxidative enzyme activities are similar in all individual samples of transitional milk from mothers of preterm infants. ConclusionsOur results indicate that transitional milk of mothers of preterm infants shows slow individual variations in antioxidative defence composition; therefore, it can be used in human milk banks.",
publisher = "Wiley, Hoboken",
journal = "Journal of Paediatrics and Child Health",
title = "Redox properties of transitional milk from mothers of preterm infants",
volume = "54",
number = "2",
pages = "160-164",
doi = "10.1111/jpc.13676"
}

Minić, S., Jesic, M., Đurović, D., Miletić, S., Lugonja, N., Marinković, V., Nikolić-Kokić, A., Spasić, S.,& Vrvić, M.. (2018). Redox properties of transitional milk from mothers of preterm infants. in Journal of Paediatrics and Child Health
Wiley, Hoboken., 54(2), 160-164.
https://doi.org/10.1111/jpc.13676

Minić S, Jesic M, Đurović D, Miletić S, Lugonja N, Marinković V, Nikolić-Kokić A, Spasić S, Vrvić M. Redox properties of transitional milk from mothers of preterm infants. in Journal of Paediatrics and Child Health. 2018;54(2):160-164.
doi:10.1111/jpc.13676 .

Minić, Simeon, Jesic, Milos, Đurović, Dijana, Miletić, Srđan, Lugonja, Nikoleta, Marinković, Vesna, Nikolić-Kokić, Aleksandra, Spasić, Snežana, Vrvić, Miroslav, "Redox properties of transitional milk from mothers of preterm infants" in Journal of Paediatrics and Child Health, 54, no. 2 (2018):160-164,
https://doi.org/10.1111/jpc.13676 . .