TY  - JOUR
AU  - Šaraba, Vladimir
AU  - Popović, Slađana
AU  - Obradović, Vesna
AU  - Štrbački, Jana
AU  - Gajić, Violeta
AU  - Vulić, Predrag
AU  - Subakov-Simić, Gordana
AU  - Krunić, Olivera
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3359
AB  - Investigations focusing on wellhead encrustations within select zones of emergence of mineral water, in different hydrogeological provinces, were conducted from 2014 to 2017 in Serbia. They included: well BB-1 in Bogatić (Inner Dinaric Alps of western Serbia), wells LB-4 and LB-5 in Lukovska Banja (Šumadija-Kopaonik-Kosovo Province), and wells VG-2 and VG-3 in Vranjska Banja (Serbian Crystalline Core). The studied occurrences belong to the group of hot mineral waters, with temperatures ranging from 62°C to 93.9°C, and total dissolved solids (TDS) from 600 to 1267 mg/L. They are mildly acidic to mildly alkaline (pH 6.6 – 8.0) and their genetic types are HCO3 --Na++K+ (Bogatić and Lukovska Banja) and SO4 2-, HCO3 --Na++K+ (Vranjska Banja). Macroscopic, optical and diffraction assessment revealed that the encrustations comprised calcite and aragonite minerals, along with some local quartz, muscovite, albite and clinochlore. The conclusion was that there was a predominant presence of calcium carbonate in all the occurrences, represented by crystalline calcite grains, characteristically birefractive, and dark, semitranslucent grains in the form of microcrystalline calcite highly stained by iron hydroxides (Bogatić), as well as crystalline calcite with radially developed carbonate - aragonite (Lukovska Banja) and rod-like and filamentous aggregates of aragonite and calcite, highly translucent in places (Vranjska Banja).The texture of the samples exhibits alternating horizontal or wavy carbonate laminae. In Vranjska Banja, they include thin micritic to medium crystalline laminae and laminae composed of aggregates of aragonite needles in the form of bunch. In Bogatić and Lukovska Banja, there are thick micritic and highly porous laminae, 0.025 to 1 mm thick, or interlayers of a coarser crystalline structure. Apart from encrustations, all the occurrences exhibit phototrophic microbial mats, which were analyzed by scanning electron microscopy. The onset and progress of encrustation are primarily influenced by the physical and chemical composition of the mineral water (T, pH, TDS, HCO3 -, Ca2+, CO2, etc.), as corroborated by hydrogeochemical modeling software PHREEQC 3.4.0. In addition, phototrophic biofilms might be contributing to the creation and accumulation of minerals in the encrustations.
PB  - Institut za Geoloska Istrazivanja
T2  - Geologia Croatica
T1  - Macroscopic, optical and diffraction assessment of encrustations and SEM analyses of phototrophic microbial mats from wellheads and select zones of emergence of mineral water in Serbia
VL  - 72
IS  - 2
SP  - 145
EP  - 162
DO  - 10.4154/gc.2019.09
ER  - 

@article{
author = "Šaraba, Vladimir and Popović, Slađana and Obradović, Vesna and Štrbački, Jana and Gajić, Violeta and Vulić, Predrag and Subakov-Simić, Gordana and Krunić, Olivera",
year = "2019",
abstract = "Investigations focusing on wellhead encrustations within select zones of emergence of mineral water, in different hydrogeological provinces, were conducted from 2014 to 2017 in Serbia. They included: well BB-1 in Bogatić (Inner Dinaric Alps of western Serbia), wells LB-4 and LB-5 in Lukovska Banja (Šumadija-Kopaonik-Kosovo Province), and wells VG-2 and VG-3 in Vranjska Banja (Serbian Crystalline Core). The studied occurrences belong to the group of hot mineral waters, with temperatures ranging from 62°C to 93.9°C, and total dissolved solids (TDS) from 600 to 1267 mg/L. They are mildly acidic to mildly alkaline (pH 6.6 – 8.0) and their genetic types are HCO3 --Na++K+ (Bogatić and Lukovska Banja) and SO4 2-, HCO3 --Na++K+ (Vranjska Banja). Macroscopic, optical and diffraction assessment revealed that the encrustations comprised calcite and aragonite minerals, along with some local quartz, muscovite, albite and clinochlore. The conclusion was that there was a predominant presence of calcium carbonate in all the occurrences, represented by crystalline calcite grains, characteristically birefractive, and dark, semitranslucent grains in the form of microcrystalline calcite highly stained by iron hydroxides (Bogatić), as well as crystalline calcite with radially developed carbonate - aragonite (Lukovska Banja) and rod-like and filamentous aggregates of aragonite and calcite, highly translucent in places (Vranjska Banja).The texture of the samples exhibits alternating horizontal or wavy carbonate laminae. In Vranjska Banja, they include thin micritic to medium crystalline laminae and laminae composed of aggregates of aragonite needles in the form of bunch. In Bogatić and Lukovska Banja, there are thick micritic and highly porous laminae, 0.025 to 1 mm thick, or interlayers of a coarser crystalline structure. Apart from encrustations, all the occurrences exhibit phototrophic microbial mats, which were analyzed by scanning electron microscopy. The onset and progress of encrustation are primarily influenced by the physical and chemical composition of the mineral water (T, pH, TDS, HCO3 -, Ca2+, CO2, etc.), as corroborated by hydrogeochemical modeling software PHREEQC 3.4.0. In addition, phototrophic biofilms might be contributing to the creation and accumulation of minerals in the encrustations.",
publisher = "Institut za Geoloska Istrazivanja",
journal = "Geologia Croatica",
title = "Macroscopic, optical and diffraction assessment of encrustations and SEM analyses of phototrophic microbial mats from wellheads and select zones of emergence of mineral water in Serbia",
volume = "72",
number = "2",
pages = "145-162",
doi = "10.4154/gc.2019.09"
}

Šaraba, V., Popović, S., Obradović, V., Štrbački, J., Gajić, V., Vulić, P., Subakov-Simić, G.,& Krunić, O.. (2019). Macroscopic, optical and diffraction assessment of encrustations and SEM analyses of phototrophic microbial mats from wellheads and select zones of emergence of mineral water in Serbia. in Geologia Croatica
Institut za Geoloska Istrazivanja., 72(2), 145-162.
https://doi.org/10.4154/gc.2019.09

Šaraba V, Popović S, Obradović V, Štrbački J, Gajić V, Vulić P, Subakov-Simić G, Krunić O. Macroscopic, optical and diffraction assessment of encrustations and SEM analyses of phototrophic microbial mats from wellheads and select zones of emergence of mineral water in Serbia. in Geologia Croatica. 2019;72(2):145-162.
doi:10.4154/gc.2019.09 .

Šaraba, Vladimir, Popović, Slađana, Obradović, Vesna, Štrbački, Jana, Gajić, Violeta, Vulić, Predrag, Subakov-Simić, Gordana, Krunić, Olivera, "Macroscopic, optical and diffraction assessment of encrustations and SEM analyses of phototrophic microbial mats from wellheads and select zones of emergence of mineral water in Serbia" in Geologia Croatica, 72, no. 2 (2019):145-162,
https://doi.org/10.4154/gc.2019.09 . .

TY  - JOUR
AU  - Saraba, Vladimir
AU  - Popović, Slađana
AU  - Krunić, Olivera
AU  - Subakov-Simić, Gordana
AU  - Kljajic, Zeljko
AU  - Lazić, Milojko M.
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2100
AB  - Phototropic microorganisms in thermal mats had not attracted enough attention in Serbia in the past. The research presented in this paper is the first to address biofilms at selected occurrences of mineral water in Serbia (Bogatic, Metkovic and Belotic in the Macva District as well as Radaljska Banja, Lukovska Banja and Vranjska Banja) (in Serbian, banja denotes a spa town). The study period is from 2014 to 2016. The temperatures of the studied occurrences are in the 30-93.9 degrees C range and the chemical composition corresponds to the HCO3- -Na++ K+ and SO42-, HCO3--Na++ K+ types of mineral water. One of the objectives was to examine the condition of wellheads (incrustation, biofouling and corrosion), focusing on the presence of phototrophic microorganisms whose metabolites may lead to changes in mineral water quality, yield and chemistry. Analysis by light and scanning electron microscopy provides insight into the diversity of phototrophic microorganisms that populate biofilms near the points of emergence and on wellhead. The research reveals the presence of representatives of three groups of phototrophic microorganisms: Cyanobacteria, Chlorophyta and Bacillariophyta. Cyanobacteria were the most numerous, dominated by the order Oscillatoriales with ten recorded genera, of which Leptolynbgya and Phormidium feature the largest numbers of species. The order Chroococcales are the second most numerous, with seven recorded genera, of which Gloeocapsa and Synechocystisare the most noteworthy. The largest numbers of representative taxa were noted at Radaljska Banja and Vranjska Banja, where mineral waters originate from lithostratigraphic units of igneous origin, followed by mineral water occurrences in karst aquifers (Bogatic and Belotic) and an intergranular aquifer (Metkovic). The smallest number of identified phototrophic microorganisms is noted at Lukovska Banja, where the origin is associated with a fractured aquifer formed in sedimentary and metamorphic rocks, with occasional diabase interbeds. Although many representative taxa are found on only one of the studied localities, such as Synechococcus bigranulatus and Pseudanabaena thermalis, those of the genera Leptolyngbya, Phormidium and Cosmarium laeve, as well as genera of the phylum Bacillariophyta, occur at several sampling sites. Principal component analysis (PCA) was used to show the relationship between documented cyanobacterial and algal taxa and environmental parameters.
AB  - Fototrofni mikroorganizmi v oblogah termalnih izvirov v Srbiji v preteklosti niso bili deležni posebne pozornosti. V članku predstavljena raziskava je prva, ki obravnava biofilme na izbranih mestih izvirov mineralnih vod v Srbiji (Bogatić, Metković in Belotić v Mačvi ter Radaljska Banja, Lukovska Banja in Vranjska Banja) (v srbskem jeziku je banja izraz za toplice). Preučevanje je potekalo med letoma 2014 in 2016. Temperature obravnavanih voda se gibljejo med 30 in 93,9 °C, glede na kemijsko sestavo pa jih uvrščamo v HCO3 − – Na++ K+ in SO4 2−, HCO3 − – Na++K+ tipe mineralne vode. Eden od ciljev raziskave je bil proučevati razmere na ustjih vrtin (inkrustacija, obrast in korozija) in se še posebej posvetiti navzočnosti fototrofnih mikroorganizmov, katerih metaboliti lahko spremenijo kakovost, izdatnost in kemizem mineralnih vod. Z analizo slik s svetlobnim in vrstičnim elektronskim mikroskopom lahko podrobneje spoznamo raznolikost fototrofnih organizmov, ki sestavljajo biofilme na mestih izvirov in na ustjih vrtin. Raziskava je pokazala obstoj predstavnikov treh skupin fototrofnih mikroorganizmov: cianobakterij ter predstavnikov debel Chlorophyta in Bacillariophyta. Cianobakterije so bile najbolj številčne, prevladovali so osebki reda Oscillatoriales z desetimi ugotovljenimi rodovi, od katerih je bilo največje število vrst iz rodov Leptolynbgya in Phormidium. Red Chroococcales je bil s sedmimi zabeleženimi rodovi drugi najbolj številčen, prevladovala sta Gloeocapsa in Synechocystis. Največreprezentativnih taksonov je bilo v Radaljski Banji in Vranjski Banji, kjer mineralne vode izvirajo iz litostratigrafskih enot vulkanskega izvora, sledijo mineralne vode iz kraškega (Bogatić in Belotić) in medzrnskega vodonosnika (Metković). Najmanjfototrofnih organizmov je bilo v vzorcu iz Lukovske Banje, kjer vode izvirajo iz razpoklinskih vodonosnikov v sedimentnih in metamorfnih kamninah z vmesnimi lečami diabaza. Čeprav soštevilne reprezentativne taksone našli le na eni od proučevanih lokacij, kot na primer Synechococcus bigranulatus in Pseudanabaena thermalis, se tisti iz rodu Leptolyngbya, Phormidium in Cosmarium laeve in predstavniki več rodov, ki pripadajo deblu Bacillariophyta, pojavljajo na več vzorčnih mestih. Za ugotavljanje odvisnosti med taksoni cianobakterij in alg ter okoljskimi parametri so uporabili analizo glavnih komponent.
PB  - Zalozba Z R C
T2  - Acta Carsologica
T1  - Mineral waters of Serbia and development of phototrophic microbial communities near points of emergence and on wellheads
VL  - 46
IS  - 2-3
SP  - 295
EP  - 316
DO  - 10.3986/ac.v46i2-3.4961
ER  - 

@article{
author = "Saraba, Vladimir and Popović, Slađana and Krunić, Olivera and Subakov-Simić, Gordana and Kljajic, Zeljko and Lazić, Milojko M.",
year = "2017",
abstract = "Phototropic microorganisms in thermal mats had not attracted enough attention in Serbia in the past. The research presented in this paper is the first to address biofilms at selected occurrences of mineral water in Serbia (Bogatic, Metkovic and Belotic in the Macva District as well as Radaljska Banja, Lukovska Banja and Vranjska Banja) (in Serbian, banja denotes a spa town). The study period is from 2014 to 2016. The temperatures of the studied occurrences are in the 30-93.9 degrees C range and the chemical composition corresponds to the HCO3- -Na++ K+ and SO42-, HCO3--Na++ K+ types of mineral water. One of the objectives was to examine the condition of wellheads (incrustation, biofouling and corrosion), focusing on the presence of phototrophic microorganisms whose metabolites may lead to changes in mineral water quality, yield and chemistry. Analysis by light and scanning electron microscopy provides insight into the diversity of phototrophic microorganisms that populate biofilms near the points of emergence and on wellhead. The research reveals the presence of representatives of three groups of phototrophic microorganisms: Cyanobacteria, Chlorophyta and Bacillariophyta. Cyanobacteria were the most numerous, dominated by the order Oscillatoriales with ten recorded genera, of which Leptolynbgya and Phormidium feature the largest numbers of species. The order Chroococcales are the second most numerous, with seven recorded genera, of which Gloeocapsa and Synechocystisare the most noteworthy. The largest numbers of representative taxa were noted at Radaljska Banja and Vranjska Banja, where mineral waters originate from lithostratigraphic units of igneous origin, followed by mineral water occurrences in karst aquifers (Bogatic and Belotic) and an intergranular aquifer (Metkovic). The smallest number of identified phototrophic microorganisms is noted at Lukovska Banja, where the origin is associated with a fractured aquifer formed in sedimentary and metamorphic rocks, with occasional diabase interbeds. Although many representative taxa are found on only one of the studied localities, such as Synechococcus bigranulatus and Pseudanabaena thermalis, those of the genera Leptolyngbya, Phormidium and Cosmarium laeve, as well as genera of the phylum Bacillariophyta, occur at several sampling sites. Principal component analysis (PCA) was used to show the relationship between documented cyanobacterial and algal taxa and environmental parameters., Fototrofni mikroorganizmi v oblogah termalnih izvirov v Srbiji v preteklosti niso bili deležni posebne pozornosti. V članku predstavljena raziskava je prva, ki obravnava biofilme na izbranih mestih izvirov mineralnih vod v Srbiji (Bogatić, Metković in Belotić v Mačvi ter Radaljska Banja, Lukovska Banja in Vranjska Banja) (v srbskem jeziku je banja izraz za toplice). Preučevanje je potekalo med letoma 2014 in 2016. Temperature obravnavanih voda se gibljejo med 30 in 93,9 °C, glede na kemijsko sestavo pa jih uvrščamo v HCO3 − – Na++ K+ in SO4 2−, HCO3 − – Na++K+ tipe mineralne vode. Eden od ciljev raziskave je bil proučevati razmere na ustjih vrtin (inkrustacija, obrast in korozija) in se še posebej posvetiti navzočnosti fototrofnih mikroorganizmov, katerih metaboliti lahko spremenijo kakovost, izdatnost in kemizem mineralnih vod. Z analizo slik s svetlobnim in vrstičnim elektronskim mikroskopom lahko podrobneje spoznamo raznolikost fototrofnih organizmov, ki sestavljajo biofilme na mestih izvirov in na ustjih vrtin. Raziskava je pokazala obstoj predstavnikov treh skupin fototrofnih mikroorganizmov: cianobakterij ter predstavnikov debel Chlorophyta in Bacillariophyta. Cianobakterije so bile najbolj številčne, prevladovali so osebki reda Oscillatoriales z desetimi ugotovljenimi rodovi, od katerih je bilo največje število vrst iz rodov Leptolynbgya in Phormidium. Red Chroococcales je bil s sedmimi zabeleženimi rodovi drugi najbolj številčen, prevladovala sta Gloeocapsa in Synechocystis. Največreprezentativnih taksonov je bilo v Radaljski Banji in Vranjski Banji, kjer mineralne vode izvirajo iz litostratigrafskih enot vulkanskega izvora, sledijo mineralne vode iz kraškega (Bogatić in Belotić) in medzrnskega vodonosnika (Metković). Najmanjfototrofnih organizmov je bilo v vzorcu iz Lukovske Banje, kjer vode izvirajo iz razpoklinskih vodonosnikov v sedimentnih in metamorfnih kamninah z vmesnimi lečami diabaza. Čeprav soštevilne reprezentativne taksone našli le na eni od proučevanih lokacij, kot na primer Synechococcus bigranulatus in Pseudanabaena thermalis, se tisti iz rodu Leptolyngbya, Phormidium in Cosmarium laeve in predstavniki več rodov, ki pripadajo deblu Bacillariophyta, pojavljajo na več vzorčnih mestih. Za ugotavljanje odvisnosti med taksoni cianobakterij in alg ter okoljskimi parametri so uporabili analizo glavnih komponent.",
publisher = "Zalozba Z R C",
journal = "Acta Carsologica",
title = "Mineral waters of Serbia and development of phototrophic microbial communities near points of emergence and on wellheads",
volume = "46",
number = "2-3",
pages = "295-316",
doi = "10.3986/ac.v46i2-3.4961"
}

Saraba, V., Popović, S., Krunić, O., Subakov-Simić, G., Kljajic, Z.,& Lazić, M. M.. (2017). Mineral waters of Serbia and development of phototrophic microbial communities near points of emergence and on wellheads. in Acta Carsologica
Zalozba Z R C., 46(2-3), 295-316.
https://doi.org/10.3986/ac.v46i2-3.4961

Saraba V, Popović S, Krunić O, Subakov-Simić G, Kljajic Z, Lazić MM. Mineral waters of Serbia and development of phototrophic microbial communities near points of emergence and on wellheads. in Acta Carsologica. 2017;46(2-3):295-316.
doi:10.3986/ac.v46i2-3.4961 .

Saraba, Vladimir, Popović, Slađana, Krunić, Olivera, Subakov-Simić, Gordana, Kljajic, Zeljko, Lazić, Milojko M., "Mineral waters of Serbia and development of phototrophic microbial communities near points of emergence and on wellheads" in Acta Carsologica, 46, no. 2-3 (2017):295-316,
https://doi.org/10.3986/ac.v46i2-3.4961 . .