TY  - JOUR
AU  - Čairović, Aleksandra D.
AU  - Stanimirović, Dragan D.
AU  - Krajnović, Tamara T.
AU  - Dojčinović, Biljana
AU  - Maksimović, Vesna M.
AU  - Cvijović-Alagić, Ivana Lj.
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2993
AB  - The biological quality and chemical composition of alloys used in dental practice change during heat treatment.
Often the residues of the previous cast are not disposed of but are reused and recycled until consumed. Thus, manufactured dental restorations have modified biological quality and chemical composition, and compromised biocompatibility. The aim of this study was to investigate the influence of repeated casting on the cytotoxicity of the silver-palladium (Ag-Pd) alloy. Our results showed that repeated casting of the Ag-Pd dental alloy affected its biocompatibility by promoting toxicity against transformed fibroblasts in a contact-independent manner. A strong decrease in cell proliferation, induction of senescence and massive cell death were observed in cultures exposed only to a medium previously incubated with dental alloy samples. The obtained data indicated that toxicity mediated by the accumulation of the Ag, Pd, Cu and Zn cations released from the Ag-Pd material was enhanced by recasting. The induction of cell senescence and subsequent apoptotic
and necrotic death were accompanied by amplified intracellular production of reactive oxygen and nitrogen species, suggesting their involvement in the cell destruction process. Therefore, compromised biocompatibility after recasting with the Ag-Pd alloy can be the cause of serious local cell destruction, as observed in clinical practice.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death
VL  - 71
IS  - 2
SP  - 347
EP  - 356
DO  - 10.2298/ABS190305017C
ER  - 

@article{
author = "Čairović, Aleksandra D. and Stanimirović, Dragan D. and Krajnović, Tamara T. and Dojčinović, Biljana and Maksimović, Vesna M. and Cvijović-Alagić, Ivana Lj.",
year = "2019",
abstract = "The biological quality and chemical composition of alloys used in dental practice change during heat treatment.
Often the residues of the previous cast are not disposed of but are reused and recycled until consumed. Thus, manufactured dental restorations have modified biological quality and chemical composition, and compromised biocompatibility. The aim of this study was to investigate the influence of repeated casting on the cytotoxicity of the silver-palladium (Ag-Pd) alloy. Our results showed that repeated casting of the Ag-Pd dental alloy affected its biocompatibility by promoting toxicity against transformed fibroblasts in a contact-independent manner. A strong decrease in cell proliferation, induction of senescence and massive cell death were observed in cultures exposed only to a medium previously incubated with dental alloy samples. The obtained data indicated that toxicity mediated by the accumulation of the Ag, Pd, Cu and Zn cations released from the Ag-Pd material was enhanced by recasting. The induction of cell senescence and subsequent apoptotic
and necrotic death were accompanied by amplified intracellular production of reactive oxygen and nitrogen species, suggesting their involvement in the cell destruction process. Therefore, compromised biocompatibility after recasting with the Ag-Pd alloy can be the cause of serious local cell destruction, as observed in clinical practice.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death",
volume = "71",
number = "2",
pages = "347-356",
doi = "10.2298/ABS190305017C"
}

Čairović, A. D., Stanimirović, D. D., Krajnović, T. T., Dojčinović, B., Maksimović, V. M.,& Cvijović-Alagić, I. Lj.. (2019). Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death. in Archives of Biological Sciences
Serbian Biological Society., 71(2), 347-356.
https://doi.org/10.2298/ABS190305017C

Čairović AD, Stanimirović DD, Krajnović TT, Dojčinović B, Maksimović VM, Cvijović-Alagić IL. Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death. in Archives of Biological Sciences. 2019;71(2):347-356.
doi:10.2298/ABS190305017C .

Čairović, Aleksandra D., Stanimirović, Dragan D., Krajnović, Tamara T., Dojčinović, Biljana, Maksimović, Vesna M., Cvijović-Alagić, Ivana Lj., "Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death" in Archives of Biological Sciences, 71, no. 2 (2019):347-356,
https://doi.org/10.2298/ABS190305017C . .

TY  - JOUR
AU  - Kaluđerović, Goran N.
AU  - Bulatović, Mirna
AU  - Krajnović, Tamara T.
AU  - Paschke, Reinhard
AU  - Zmejkovski, Bojana
AU  - Maksimović-Ivanić, Danijela D.
AU  - Mijatović, Sanja
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2222
AB  - Synthesis of platinum(II) conjugate with acetylated betulinic acid tris(hydroxymethyl) aminomethane ester (BATRIS) is presented (BATRISPt). HR-ESI-MS and multinuclear NMR spectroscopy, as well as elemental analysis were used for characterization of BATRISPt. Cytotoxicity (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), crystal violet (CV), and sulforhodamine B (SRB) assays) of BA, BATRIS, BATRISPt, and cisplatin were assessed on seven different tumor cell lines: melanoma B16, colon HCT116 and DLD-1, adenocarcinoma HeLa, breast MCF-7, and anaplastic thyroid tumor 8505C and SW1736; as well as normal MRC-5 fibroblasts. Furthermore, the effect of the mentioned compounds on the apoptosis (Annexin V/PI assay) and autophagy induction (acridine orange (AO) assay) as well as caspase 3, 8, and 9 activation were investigated on the selected B16 melanoma cell line. BATRISPt showed lower activity than BA, BATRIS, or cisplatin. All tested compounds triggered apoptosis in B16 cells. Induction of autophagy was observed in B16 cells exposed only to BATRIS. On the other hand, new conjugate activates caspases 8 and 9 in B16 cells with higher impact than BATRIS or cisplatin alone.
PB  - MDPI
T2  - Inorganics
T1  - (18-Crown-6)potassium(I) trichlorido[28-acetyl-3- (tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and in vitro antitumor activity
VL  - 5
IS  - 3
DO  - 10.3390/inorganics5030056
ER  - 

@article{
author = "Kaluđerović, Goran N. and Bulatović, Mirna and Krajnović, Tamara T. and Paschke, Reinhard and Zmejkovski, Bojana and Maksimović-Ivanić, Danijela D. and Mijatović, Sanja",
year = "2017",
abstract = "Synthesis of platinum(II) conjugate with acetylated betulinic acid tris(hydroxymethyl) aminomethane ester (BATRIS) is presented (BATRISPt). HR-ESI-MS and multinuclear NMR spectroscopy, as well as elemental analysis were used for characterization of BATRISPt. Cytotoxicity (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), crystal violet (CV), and sulforhodamine B (SRB) assays) of BA, BATRIS, BATRISPt, and cisplatin were assessed on seven different tumor cell lines: melanoma B16, colon HCT116 and DLD-1, adenocarcinoma HeLa, breast MCF-7, and anaplastic thyroid tumor 8505C and SW1736; as well as normal MRC-5 fibroblasts. Furthermore, the effect of the mentioned compounds on the apoptosis (Annexin V/PI assay) and autophagy induction (acridine orange (AO) assay) as well as caspase 3, 8, and 9 activation were investigated on the selected B16 melanoma cell line. BATRISPt showed lower activity than BA, BATRIS, or cisplatin. All tested compounds triggered apoptosis in B16 cells. Induction of autophagy was observed in B16 cells exposed only to BATRIS. On the other hand, new conjugate activates caspases 8 and 9 in B16 cells with higher impact than BATRIS or cisplatin alone.",
publisher = "MDPI",
journal = "Inorganics",
title = "(18-Crown-6)potassium(I) trichlorido[28-acetyl-3- (tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and in vitro antitumor activity",
volume = "5",
number = "3",
doi = "10.3390/inorganics5030056"
}

Kaluđerović, G. N., Bulatović, M., Krajnović, T. T., Paschke, R., Zmejkovski, B., Maksimović-Ivanić, D. D.,& Mijatović, S.. (2017). (18-Crown-6)potassium(I) trichlorido[28-acetyl-3- (tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and in vitro antitumor activity. in Inorganics
MDPI., 5(3).
https://doi.org/10.3390/inorganics5030056

Kaluđerović GN, Bulatović M, Krajnović TT, Paschke R, Zmejkovski B, Maksimović-Ivanić DD, Mijatović S. (18-Crown-6)potassium(I) trichlorido[28-acetyl-3- (tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and in vitro antitumor activity. in Inorganics. 2017;5(3).
doi:10.3390/inorganics5030056 .

Kaluđerović, Goran N., Bulatović, Mirna, Krajnović, Tamara T., Paschke, Reinhard, Zmejkovski, Bojana, Maksimović-Ivanić, Danijela D., Mijatović, Sanja, "(18-Crown-6)potassium(I) trichlorido[28-acetyl-3- (tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and in vitro antitumor activity" in Inorganics, 5, no. 3 (2017),
https://doi.org/10.3390/inorganics5030056 . .