TY  - JOUR
AU  - Jovanovic, Jovana
AU  - Kolarevic, Stoimir
AU  - Miloskovic, Aleksandra
AU  - Radojkovic, Natasa
AU  - Simić, Vladica
AU  - Dojčinović, Biljana
AU  - Kracun-Kolarevic, Margareta
AU  - Paunović, Momir
AU  - Kostic, Jovana
AU  - Sunjog, Karolina
AU  - Timilijic, Jovana
AU  - Đorđević, Jelena B.
AU  - Gačić, Zoran
AU  - Zegura, Bojana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2468
AB  - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
PB  - Elsevier
T2  - Science of the Total Environment
T1  - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
VL  - 621
SP  - 1289
EP  - 1299
DO  - 10.1016/j.scitotenv.2017.10.099
ER  - 

@article{
author = "Jovanovic, Jovana and Kolarevic, Stoimir and Miloskovic, Aleksandra and Radojkovic, Natasa and Simić, Vladica and Dojčinović, Biljana and Kracun-Kolarevic, Margareta and Paunović, Momir and Kostic, Jovana and Sunjog, Karolina and Timilijic, Jovana and Đorđević, Jelena B. and Gačić, Zoran and Zegura, Bojana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.",
publisher = "Elsevier",
journal = "Science of the Total Environment",
title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ",
volume = "621",
pages = "1289-1299",
doi = "10.1016/j.scitotenv.2017.10.099"
}

Jovanovic, J., Kolarevic, S., Miloskovic, A., Radojkovic, N., Simić, V., Dojčinović, B., Kracun-Kolarevic, M., Paunović, M., Kostic, J., Sunjog, K., Timilijic, J., Đorđević, J. B., Gačić, Z., Zegura, B.,& Vukovic-Gacic, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment
Elsevier., 621, 1289-1299.
https://doi.org/10.1016/j.scitotenv.2017.10.099

Jovanovic J, Kolarevic S, Miloskovic A, Radojkovic N, Simić V, Dojčinović B, Kracun-Kolarevic M, Paunović M, Kostic J, Sunjog K, Timilijic J, Đorđević JB, Gačić Z, Zegura B, Vukovic-Gacic B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment. 2018;621:1289-1299.
doi:10.1016/j.scitotenv.2017.10.099 .

Jovanovic, Jovana, Kolarevic, Stoimir, Miloskovic, Aleksandra, Radojkovic, Natasa, Simić, Vladica, Dojčinović, Biljana, Kracun-Kolarevic, Margareta, Paunović, Momir, Kostic, Jovana, Sunjog, Karolina, Timilijic, Jovana, Đorđević, Jelena B., Gačić, Zoran, Zegura, Bojana, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of the Total Environment, 621 (2018):1289-1299,
https://doi.org/10.1016/j.scitotenv.2017.10.099 . .

TY  - JOUR
AU  - Kolarevic, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kracun-Kolarevic, Margareta
AU  - Kostic, Jovana
AU  - Simonović, Predrag
AU  - Simić, Vladica
AU  - Miloskovic, Aleksandra
AU  - Reischer, Georg
AU  - Farnleitner, Andreas
AU  - Gačić, Zoran
AU  - Milacic, Radmila
AU  - Zuliani, Tea
AU  - Vidmar, Janja
AU  - Pergal, Marija
AU  - Piria, Marina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1920
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
PB  - Public Library of Science (PLoS)
T2  - Plos One
T1  - Evaluation of Genotoxic Pressure along the Sava River
VL  - 11
IS  - 9
DO  - 10.1371/journal.pone.0162450
ER  - 

@article{
author = "Kolarevic, Stoimir and Aborgiba, Mustafa and Kracun-Kolarevic, Margareta and Kostic, Jovana and Simonović, Predrag and Simić, Vladica and Miloskovic, Aleksandra and Reischer, Georg and Farnleitner, Andreas and Gačić, Zoran and Milacic, Radmila and Zuliani, Tea and Vidmar, Janja and Pergal, Marija and Piria, Marina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
publisher = "Public Library of Science (PLoS)",
journal = "Plos One",
title = "Evaluation of Genotoxic Pressure along the Sava River",
volume = "11",
number = "9",
doi = "10.1371/journal.pone.0162450"
}

Kolarevic, S., Aborgiba, M., Kracun-Kolarevic, M., Kostic, J., Simonović, P., Simić, V., Miloskovic, A., Reischer, G., Farnleitner, A., Gačić, Z., Milacic, R., Zuliani, T., Vidmar, J., Pergal, M., Piria, M., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of Genotoxic Pressure along the Sava River. in Plos One
Public Library of Science (PLoS)., 11(9).
https://doi.org/10.1371/journal.pone.0162450

Kolarevic S, Aborgiba M, Kracun-Kolarevic M, Kostic J, Simonović P, Simić V, Miloskovic A, Reischer G, Farnleitner A, Gačić Z, Milacic R, Zuliani T, Vidmar J, Pergal M, Piria M, Paunović M, Vukovic-Gacic B. Evaluation of Genotoxic Pressure along the Sava River. in Plos One. 2016;11(9).
doi:10.1371/journal.pone.0162450 .

Kolarevic, Stoimir, Aborgiba, Mustafa, Kracun-Kolarevic, Margareta, Kostic, Jovana, Simonović, Predrag, Simić, Vladica, Miloskovic, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milacic, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of Genotoxic Pressure along the Sava River" in Plos One, 11, no. 9 (2016),
https://doi.org/10.1371/journal.pone.0162450 . .