TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Zarić, Božidarka
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Radojčić, Marija
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3945
AB  - To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-κB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4+ lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4+ cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4+ cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.
PB  - Taylor & Francis
T2  - Redox Report
T1  - Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects
VL  - 13
IS  - 1
SP  - 17
EP  - 22
DO  - 10.1179/135100008X259088
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Zarić, Božidarka and Nešković-Konstantinović, Zora and Spasić, Snežana and Jones, David and Radojčić, Marija",
year = "2013",
abstract = "To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-κB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4+ lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4+ cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4+ cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.",
publisher = "Taylor & Francis",
journal = "Redox Report",
title = "Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects",
volume = "13",
number = "1",
pages = "17-22",
doi = "10.1179/135100008X259088"
}

Adžić, M., Nićiforović, A., Zarić, B., Nešković-Konstantinović, Z., Spasić, S., Jones, D.,& Radojčić, M.. (2013). Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects. in Redox Report
Taylor & Francis., 13(1), 17-22.
https://doi.org/10.1179/135100008X259088

Adžić M, Nićiforović A, Zarić B, Nešković-Konstantinović Z, Spasić S, Jones D, Radojčić M. Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects. in Redox Report. 2013;13(1):17-22.
doi:10.1179/135100008X259088 .

Adžić, Miroslav, Nićiforović, Ana, Zarić, Božidarka, Nešković-Konstantinović, Zora, Spasić, Snežana, Jones, David, Radojčić, Marija, "Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects" in Redox Report, 13, no. 1 (2013):17-22,
https://doi.org/10.1179/135100008X259088 . .

TY  - JOUR
AU  - Nićiforović, A.
AU  - Adžić, Miroslav
AU  - Spasić, Snežana
AU  - Radojčić, M. B.
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/372
AB  - Most experimental models, including cell culture studies, have demonstrated that over-expression of manganese superoxide dismutase (MnSOD) in cells bearing a carcinoma phenotype has anti-proliferative and tumour suppression characteristics. In contrast, when cervical carcinoma biopsies express MnSOD, there is a poor prognosis and resistance to radiation therapy. The results herein indicate that human cervical adenocarcinoma (HeLaS3) cells have increased MnSOD activity (up to 50 % of the total SOD activity) due to low expression of its repressor p53 and a high level of oxidative stress arising from the cell culture conditions. High MnSOD activity may be related to HeLaS3 cell radioresistance, illustrated by a high IC50 of 3.4 Gy and by a relatively high level of cell viability after gamma irradiation. In contrast to MnSOD activity, cytosolic CuZnSOD activity decreased after ionising radiation. The catalase (Cat) activity was unchanged. IR also increased the nitric oxide synthase (NOS) activity. Such conditions lead to increased concentrations of the superoxide radical, hydrogen peroxide and NO., which together may be responsible for the decreased expression of NF-κB and unaltered Cat activity. Therefore, the disturbed redox balance within HeLaS3 cells may be responsible for the cytotoxicity observed at higher irradiation doses. It could be concluded that inhibition of the CuZnSOD activity may be an important target for the selective killing of radioresistant cancer cells.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?
VL  - 72
IS  - 10
SP  - 945
EP  - 952
DO  - 10.2298/JSC0710945N
ER  - 

@article{
author = "Nićiforović, A. and Adžić, Miroslav and Spasić, Snežana and Radojčić, M. B.",
year = "2007",
abstract = "Most experimental models, including cell culture studies, have demonstrated that over-expression of manganese superoxide dismutase (MnSOD) in cells bearing a carcinoma phenotype has anti-proliferative and tumour suppression characteristics. In contrast, when cervical carcinoma biopsies express MnSOD, there is a poor prognosis and resistance to radiation therapy. The results herein indicate that human cervical adenocarcinoma (HeLaS3) cells have increased MnSOD activity (up to 50 % of the total SOD activity) due to low expression of its repressor p53 and a high level of oxidative stress arising from the cell culture conditions. High MnSOD activity may be related to HeLaS3 cell radioresistance, illustrated by a high IC50 of 3.4 Gy and by a relatively high level of cell viability after gamma irradiation. In contrast to MnSOD activity, cytosolic CuZnSOD activity decreased after ionising radiation. The catalase (Cat) activity was unchanged. IR also increased the nitric oxide synthase (NOS) activity. Such conditions lead to increased concentrations of the superoxide radical, hydrogen peroxide and NO., which together may be responsible for the decreased expression of NF-κB and unaltered Cat activity. Therefore, the disturbed redox balance within HeLaS3 cells may be responsible for the cytotoxicity observed at higher irradiation doses. It could be concluded that inhibition of the CuZnSOD activity may be an important target for the selective killing of radioresistant cancer cells.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?",
volume = "72",
number = "10",
pages = "945-952",
doi = "10.2298/JSC0710945N"
}

Nićiforović, A., Adžić, M., Spasić, S.,& Radojčić, M. B.. (2007). Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 72(10), 945-952.
https://doi.org/10.2298/JSC0710945N

Nićiforović A, Adžić M, Spasić S, Radojčić MB. Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?. in Journal of the Serbian Chemical Society. 2007;72(10):945-952.
doi:10.2298/JSC0710945N .

Nićiforović, A., Adžić, Miroslav, Spasić, Snežana, Radojčić, M. B., "Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?" in Journal of the Serbian Chemical Society, 72, no. 10 (2007):945-952,
https://doi.org/10.2298/JSC0710945N . .

TY  - JOUR
AU  - Niciforovic, Ana
AU  - Adžić, Miroslav
AU  - Spasić, Snežana
AU  - Radojcic, Marija B.
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/335
AB  - The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Cancer Biology & Therapy
T1  - Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells
VL  - 6
IS  - 8
SP  - 1200
EP  - 1205
UR  - https://hdl.handle.net/21.15107/rcub_cer_335
ER  - 

@article{
author = "Niciforovic, Ana and Adžić, Miroslav and Spasić, Snežana and Radojcic, Marija B.",
year = "2007",
abstract = "The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Cancer Biology & Therapy",
title = "Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells",
volume = "6",
number = "8",
pages = "1200-1205",
url = "https://hdl.handle.net/21.15107/rcub_cer_335"
}

Niciforovic, A., Adžić, M., Spasić, S.,& Radojcic, M. B.. (2007). Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells. in Cancer Biology & Therapy
Taylor & Francis Inc, Philadelphia., 6(8), 1200-1205.
https://hdl.handle.net/21.15107/rcub_cer_335

Niciforovic A, Adžić M, Spasić S, Radojcic MB. Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells. in Cancer Biology & Therapy. 2007;6(8):1200-1205.
https://hdl.handle.net/21.15107/rcub_cer_335 .

Niciforovic, Ana, Adžić, Miroslav, Spasić, Snežana, Radojcic, Marija B., "Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells" in Cancer Biology & Therapy, 6, no. 8 (2007):1200-1205,
https://hdl.handle.net/21.15107/rcub_cer_335 .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Vucić, Vesna
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Radojčić, Marija
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3965
AB  - There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappaB and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappaB and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.
PB  - Maney Publishing
T2  - Redox Report
T1  - Systemic NF-kappaB activation in blood cells of breast cancer patients
VL  - 11
IS  - 1
SP  - 39
SP  - 44
DO  - 10.1179/135100006X101002
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Vucić, Vesna and Nešković-Konstantinović, Zora and Spasić, Snežana and Jones, David and Radojčić, Marija and Spasić, Mihajlo",
year = "2006",
abstract = "There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappaB and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappaB and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.",
publisher = "Maney Publishing",
journal = "Redox Report",
title = "Systemic NF-kappaB activation in blood cells of breast cancer patients",
volume = "11",
number = "1",
pages = "39-44",
doi = "10.1179/135100006X101002"
}

Adžić, M., Nićiforović, A., Vucić, V., Nešković-Konstantinović, Z., Spasić, S., Jones, D., Radojčić, M.,& Spasić, M.. (2006). Systemic NF-kappaB activation in blood cells of breast cancer patients. in Redox Report
Maney Publishing., 11(1), 39.
https://doi.org/10.1179/135100006X101002

Adžić M, Nićiforović A, Vucić V, Nešković-Konstantinović Z, Spasić S, Jones D, Radojčić M, Spasić M. Systemic NF-kappaB activation in blood cells of breast cancer patients. in Redox Report. 2006;11(1):39.
doi:10.1179/135100006X101002 .

Adžić, Miroslav, Nićiforović, Ana, Vucić, Vesna, Nešković-Konstantinović, Zora, Spasić, Snežana, Jones, David, Radojčić, Marija, Spasić, Mihajlo, "Systemic NF-kappaB activation in blood cells of breast cancer patients" in Redox Report, 11, no. 1 (2006):39,
https://doi.org/10.1179/135100006X101002 . .