Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads
Samo za registrovane korisnike
2024
Autori
Radović, JelenaPopović, Dragana
Ćurčić, Tatjana
Veličković, Luka
![](/themes/MirageCER/images/orcid.png)
Lević, Steva
Pavlović, Vladimir
Minić, Simeon
![](/themes/MirageCER/images/orcid.png)
Nikolić, Milan
![](/themes/MirageCER/images/orcid.png)
Gligorijević, Nikola
![](/themes/MirageCER/images/orcid.png)
Članak u časopisu (Objavljena verzija)
![](/themes/MirageCER//images/creativecommons/arr.png)
Elsevier B.V.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Mercury emissions represent a significant risk to the environment and human health. Mercury is persistent and
can circulate in the environment for thousands of years, which is why treating this toxic metal is important.
Chitosan polymer is easily obtainable, and it has good mercury adsorption characteristics. This study aimed to
improve its capabilities to absorb mercury by immobilizing phycobiliproteins (PBPs) onto the surface of chitosan
beads (chitosan–PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have several
industrially essential applications. These proteins can bind heavy metals with high affinities. Protein extracts
obtained from both Arthrospira platensis, with C-phycocyanin (C-PC) as the primary protein, and Porphyra
yezoensis, with R-phycocyanin (R-PC) and R-phycoerythrin (R-PE) as the dominant PBPs, were covalently
immobilized onto chitosan beads. Beads with immobilized PBPs were characterized by scanning electron microscopy
and ...Fourier transform infrared spectroscopy. Binding analysis showed that, on average, each chitosan
bead weighed 20 mg and immobilized 63.54 μg of PBPs from Spirulina and 44.12 μg of PBPs from Porphyra.
Immobilized proteins were still in their native state, with no visible color change months after immobilization.
Chitosan–PBPs and chitosan alone were tested for mercury adsorption at pH 4 and pH 7 by atomic absorption
spectroscopy. The tested concentration range of mercury was from 1 to 70 ppm. Affinity, calculated using
Henry’s binding isotherm, of chitosan–PBPs for mercury was twice as much higher at both pH values than
chitosan alone. Furthermore, chitosan-PBP beads were able to absorb more mercury than chitosan alone. These
results showed that the covalent immobilization of PBPs onto chitosan improves its mercury adsorption characteristics
and creates a more efficient eco-friendly adsorbent to potentially remove mercury ions in the tested
concentration range from polluted waters.
Ključne reči:
Phycobiliproteins / Water pollution / Mercury / Immobilization / ChitosanIzvor:
Algal Research, 2024, 80, 103543-Izdavač:
- Elsevier
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200168 (Univerzitet u Beogradu, Hemijski fakultet) (RS-MESTD-inst-2020-200168)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200026 (Univerzitet u Beogradu, Institut za hemiju, tehnologiju i metalurgiju - IHTM) (RS-MESTD-inst-2020-200026)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200116 (Univerzitet u Beogradu, Poljoprivredni fakultet) (RS-MESTD-inst-2020-200116)
Institucija/grupa
IHTMTY - JOUR AU - Radović, Jelena AU - Popović, Dragana AU - Ćurčić, Tatjana AU - Veličković, Luka AU - Lević, Steva AU - Pavlović, Vladimir AU - Minić, Simeon AU - Nikolić, Milan AU - Gligorijević, Nikola PY - 2024 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7596 AB - Mercury emissions represent a significant risk to the environment and human health. Mercury is persistent and can circulate in the environment for thousands of years, which is why treating this toxic metal is important. Chitosan polymer is easily obtainable, and it has good mercury adsorption characteristics. This study aimed to improve its capabilities to absorb mercury by immobilizing phycobiliproteins (PBPs) onto the surface of chitosan beads (chitosan–PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have several industrially essential applications. These proteins can bind heavy metals with high affinities. Protein extracts obtained from both Arthrospira platensis, with C-phycocyanin (C-PC) as the primary protein, and Porphyra yezoensis, with R-phycocyanin (R-PC) and R-phycoerythrin (R-PE) as the dominant PBPs, were covalently immobilized onto chitosan beads. Beads with immobilized PBPs were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. Binding analysis showed that, on average, each chitosan bead weighed 20 mg and immobilized 63.54 μg of PBPs from Spirulina and 44.12 μg of PBPs from Porphyra. Immobilized proteins were still in their native state, with no visible color change months after immobilization. Chitosan–PBPs and chitosan alone were tested for mercury adsorption at pH 4 and pH 7 by atomic absorption spectroscopy. The tested concentration range of mercury was from 1 to 70 ppm. Affinity, calculated using Henry’s binding isotherm, of chitosan–PBPs for mercury was twice as much higher at both pH values than chitosan alone. Furthermore, chitosan-PBP beads were able to absorb more mercury than chitosan alone. These results showed that the covalent immobilization of PBPs onto chitosan improves its mercury adsorption characteristics and creates a more efficient eco-friendly adsorbent to potentially remove mercury ions in the tested concentration range from polluted waters. PB - Elsevier T2 - Algal Research T1 - Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads VL - 80 SP - 103543 DO - 10.1016/j.algal.2024.103543 ER -
@article{ author = "Radović, Jelena and Popović, Dragana and Ćurčić, Tatjana and Veličković, Luka and Lević, Steva and Pavlović, Vladimir and Minić, Simeon and Nikolić, Milan and Gligorijević, Nikola", year = "2024", abstract = "Mercury emissions represent a significant risk to the environment and human health. Mercury is persistent and can circulate in the environment for thousands of years, which is why treating this toxic metal is important. Chitosan polymer is easily obtainable, and it has good mercury adsorption characteristics. This study aimed to improve its capabilities to absorb mercury by immobilizing phycobiliproteins (PBPs) onto the surface of chitosan beads (chitosan–PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have several industrially essential applications. These proteins can bind heavy metals with high affinities. Protein extracts obtained from both Arthrospira platensis, with C-phycocyanin (C-PC) as the primary protein, and Porphyra yezoensis, with R-phycocyanin (R-PC) and R-phycoerythrin (R-PE) as the dominant PBPs, were covalently immobilized onto chitosan beads. Beads with immobilized PBPs were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. Binding analysis showed that, on average, each chitosan bead weighed 20 mg and immobilized 63.54 μg of PBPs from Spirulina and 44.12 μg of PBPs from Porphyra. Immobilized proteins were still in their native state, with no visible color change months after immobilization. Chitosan–PBPs and chitosan alone were tested for mercury adsorption at pH 4 and pH 7 by atomic absorption spectroscopy. The tested concentration range of mercury was from 1 to 70 ppm. Affinity, calculated using Henry’s binding isotherm, of chitosan–PBPs for mercury was twice as much higher at both pH values than chitosan alone. Furthermore, chitosan-PBP beads were able to absorb more mercury than chitosan alone. These results showed that the covalent immobilization of PBPs onto chitosan improves its mercury adsorption characteristics and creates a more efficient eco-friendly adsorbent to potentially remove mercury ions in the tested concentration range from polluted waters.", publisher = "Elsevier", journal = "Algal Research", title = "Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads", volume = "80", pages = "103543", doi = "10.1016/j.algal.2024.103543" }
Radović, J., Popović, D., Ćurčić, T., Veličković, L., Lević, S., Pavlović, V., Minić, S., Nikolić, M.,& Gligorijević, N.. (2024). Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads. in Algal Research Elsevier., 80, 103543. https://doi.org/10.1016/j.algal.2024.103543
Radović J, Popović D, Ćurčić T, Veličković L, Lević S, Pavlović V, Minić S, Nikolić M, Gligorijević N. Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads. in Algal Research. 2024;80:103543. doi:10.1016/j.algal.2024.103543 .
Radović, Jelena, Popović, Dragana, Ćurčić, Tatjana, Veličković, Luka, Lević, Steva, Pavlović, Vladimir, Minić, Simeon, Nikolić, Milan, Gligorijević, Nikola, "Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads" in Algal Research, 80 (2024):103543, https://doi.org/10.1016/j.algal.2024.103543 . .