A novel gallium activated macroporous glycidyl methacrylate based copolymer and its antimicrobial potential
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cross-linked macroporous copolymers based on glycidyl methacrylate (GMA) have
been used as sorbents for metals, support for enzymes, as biomaterials etc. In this
research GMA copolymer was prepared by copolymerization of GMA (glycidyl
methacrylate) and EGDMA (ethylene glycol dimethacrylate), in the presence of inert
component (90 mass% of cyclohexanol and 10 mass% of dodecanol), and functionalized
with diethylenetriamine (DETA). Presence of epoxy groups in GMA-based copolymers
allows chemical modifications on the polymer in order to achieve its different
applications. GMA-based copolymer was activated with 0.1 M solution of Ga(NO3)3, at
pH 3, and 25 °C [1].
Antimicrobial activity of functionalized and activated GMA-based copolymer,
GMA/Ga, was analyzed against Staphylococcus aureus (Gram positive bacteria),
Escherichia coli (Gram negative bacteria), Candida albicans (yeast) and Aspergillus
niger (fungi) [2]. The agar-well diffusion method was used to evaluate antimicrobial
...
activity of GMA/Ga polymer. Nutrient agar plates were inoculated with broth of 1 MFU
microbial suspensions. Wells of 5 mm diameter were punched in the inoculated plates
using a sterile metallic borer, and then filled with 100 μL of water solution containing
nanoparticles of GMA/Ga copolymer, at various concentrations: 10, 20, and 30 mg/mL.
Control wells were filled with 100 μL of distilled water. Inoculated plates were incubated
at 37 °C/24 h for bacteria and yeast, and at 30 °C/48 h for fungus. Antimicrobial activity
of Ga activated copolymer was evaluated by measuring the inhibition zone diameter
around the wells. The GMA/Ga copolymer displayed good antimicrobial activity against
Escherichia coli and Candida albicans, which makes it selective for Gram negative
bacteria and yeasts, and as a material that can be potentially used for biomedical
applications, in food industry etc.
Кључне речи:
copolymers / glycidyl methacrylateИзвор:
9th Conference of Young Chemists of Serbia, Book of abstracts, 2023, 153-153Издавач:
- Belgrade : Serbian Chemical Society
- Belgrade : Serbian Young Chemists’ Club
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
Институција/група
IHTMTY - CONF AU - Bulatović, Sandra AU - Tadić, Tamara AU - Marković, Bojana AU - Nedić, Natalija PY - 2023 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6824 AB - Cross-linked macroporous copolymers based on glycidyl methacrylate (GMA) have been used as sorbents for metals, support for enzymes, as biomaterials etc. In this research GMA copolymer was prepared by copolymerization of GMA (glycidyl methacrylate) and EGDMA (ethylene glycol dimethacrylate), in the presence of inert component (90 mass% of cyclohexanol and 10 mass% of dodecanol), and functionalized with diethylenetriamine (DETA). Presence of epoxy groups in GMA-based copolymers allows chemical modifications on the polymer in order to achieve its different applications. GMA-based copolymer was activated with 0.1 M solution of Ga(NO3)3, at pH 3, and 25 °C [1]. Antimicrobial activity of functionalized and activated GMA-based copolymer, GMA/Ga, was analyzed against Staphylococcus aureus (Gram positive bacteria), Escherichia coli (Gram negative bacteria), Candida albicans (yeast) and Aspergillus niger (fungi) [2]. The agar-well diffusion method was used to evaluate antimicrobial activity of GMA/Ga polymer. Nutrient agar plates were inoculated with broth of 1 MFU microbial suspensions. Wells of 5 mm diameter were punched in the inoculated plates using a sterile metallic borer, and then filled with 100 μL of water solution containing nanoparticles of GMA/Ga copolymer, at various concentrations: 10, 20, and 30 mg/mL. Control wells were filled with 100 μL of distilled water. Inoculated plates were incubated at 37 °C/24 h for bacteria and yeast, and at 30 °C/48 h for fungus. Antimicrobial activity of Ga activated copolymer was evaluated by measuring the inhibition zone diameter around the wells. The GMA/Ga copolymer displayed good antimicrobial activity against Escherichia coli and Candida albicans, which makes it selective for Gram negative bacteria and yeasts, and as a material that can be potentially used for biomedical applications, in food industry etc. PB - Belgrade : Serbian Chemical Society PB - Belgrade : Serbian Young Chemists’ Club C3 - 9th Conference of Young Chemists of Serbia, Book of abstracts T1 - A novel gallium activated macroporous glycidyl methacrylate based copolymer and its antimicrobial potential SP - 153 EP - 153 UR - https://hdl.handle.net/21.15107/rcub_cer_6824 ER -
@conference{ author = "Bulatović, Sandra and Tadić, Tamara and Marković, Bojana and Nedić, Natalija", year = "2023", abstract = "Cross-linked macroporous copolymers based on glycidyl methacrylate (GMA) have been used as sorbents for metals, support for enzymes, as biomaterials etc. In this research GMA copolymer was prepared by copolymerization of GMA (glycidyl methacrylate) and EGDMA (ethylene glycol dimethacrylate), in the presence of inert component (90 mass% of cyclohexanol and 10 mass% of dodecanol), and functionalized with diethylenetriamine (DETA). Presence of epoxy groups in GMA-based copolymers allows chemical modifications on the polymer in order to achieve its different applications. GMA-based copolymer was activated with 0.1 M solution of Ga(NO3)3, at pH 3, and 25 °C [1]. Antimicrobial activity of functionalized and activated GMA-based copolymer, GMA/Ga, was analyzed against Staphylococcus aureus (Gram positive bacteria), Escherichia coli (Gram negative bacteria), Candida albicans (yeast) and Aspergillus niger (fungi) [2]. The agar-well diffusion method was used to evaluate antimicrobial activity of GMA/Ga polymer. Nutrient agar plates were inoculated with broth of 1 MFU microbial suspensions. Wells of 5 mm diameter were punched in the inoculated plates using a sterile metallic borer, and then filled with 100 μL of water solution containing nanoparticles of GMA/Ga copolymer, at various concentrations: 10, 20, and 30 mg/mL. Control wells were filled with 100 μL of distilled water. Inoculated plates were incubated at 37 °C/24 h for bacteria and yeast, and at 30 °C/48 h for fungus. Antimicrobial activity of Ga activated copolymer was evaluated by measuring the inhibition zone diameter around the wells. The GMA/Ga copolymer displayed good antimicrobial activity against Escherichia coli and Candida albicans, which makes it selective for Gram negative bacteria and yeasts, and as a material that can be potentially used for biomedical applications, in food industry etc.", publisher = "Belgrade : Serbian Chemical Society, Belgrade : Serbian Young Chemists’ Club", journal = "9th Conference of Young Chemists of Serbia, Book of abstracts", title = "A novel gallium activated macroporous glycidyl methacrylate based copolymer and its antimicrobial potential", pages = "153-153", url = "https://hdl.handle.net/21.15107/rcub_cer_6824" }
Bulatović, S., Tadić, T., Marković, B.,& Nedić, N.. (2023). A novel gallium activated macroporous glycidyl methacrylate based copolymer and its antimicrobial potential. in 9th Conference of Young Chemists of Serbia, Book of abstracts Belgrade : Serbian Chemical Society., 153-153. https://hdl.handle.net/21.15107/rcub_cer_6824
Bulatović S, Tadić T, Marković B, Nedić N. A novel gallium activated macroporous glycidyl methacrylate based copolymer and its antimicrobial potential. in 9th Conference of Young Chemists of Serbia, Book of abstracts. 2023;:153-153. https://hdl.handle.net/21.15107/rcub_cer_6824 .
Bulatović, Sandra, Tadić, Tamara, Marković, Bojana, Nedić, Natalija, "A novel gallium activated macroporous glycidyl methacrylate based copolymer and its antimicrobial potential" in 9th Conference of Young Chemists of Serbia, Book of abstracts (2023):153-153, https://hdl.handle.net/21.15107/rcub_cer_6824 .