Combined hydrogels of starch and β-lactoglobulin as matrices for the preservation of C-phycocyanin
2023
Аутори
Jovanović, ZoranaAnnighöfer, Burkhard
Dudzinski, Daniel
Veličković, Luka
Gligorijević, Nikola
Nikolić, Milan
Brûlet, Annie
Assifaoui, Ali
Combet, Sophie
Minić, Simeon
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The color of food products is an important aspect in food industry, and its preservation
remains a big challenge. We aim to preserve the natural blue dye of C-phycocyanin (C-PC)
phycobiliprotein from Spirulina microalgae. For this purpose, we incorporated C-PC in
combined starch and β-lactoglobulin (BLG) hydrogels by using a high-pressure (HP)
process. Indeed, in thermal treatment, the color derived from C-PC is entirely lost. We
characterized the obtained HP gels by both rheology and small-angle X-ray scattering
(SAXS). Various formulations of binary (BLG/C-PC) and ternary (starch/BLG/C-PC)
systems were tested under HP up to 4,500 bar. A good preservation of the C-PC pigment
was established by mixing BLG and starch with C-PC at pH 7, with concentrations of 180,
5, and 10 mg/mL, respectively. Identical component concentrations were maintained in the
binary systems. Structure of gels was characterized by SAXS providing insight of C-PC
interactions with BLG and starch after HP... process which leads to the formation of solid
gels with larger mesh compared to two-component systems. This results in enhanced
mechanical properties, which were determined by amplitude and frequency sweep
measurements using a rheometer with applied plane/plane geometry. Therefore, adding
starch, even at small concentration, significantly improves gel visual appearance and
mechanical properties. Our study reveals that preservation through HP treatment is more
effective than high temperature treatment, as visually observed through the sustained color
integrity of C-PC blue dye.
Кључне речи:
starch / β-lactoglobulin / C-phycocyanin / food productsИзвор:
Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia, 2023, 150-150Издавач:
- Serbian Biochemical Society
Финансирање / пројекти:
- The Alliance of International Science Organizations, Project No. ANSO-CR-PP-2021-01
Институција/група
IHTMTY - CONF AU - Jovanović, Zorana AU - Annighöfer, Burkhard AU - Dudzinski, Daniel AU - Veličković, Luka AU - Gligorijević, Nikola AU - Nikolić, Milan AU - Brûlet, Annie AU - Assifaoui, Ali AU - Combet, Sophie AU - Minić, Simeon PY - 2023 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6669 AB - The color of food products is an important aspect in food industry, and its preservation remains a big challenge. We aim to preserve the natural blue dye of C-phycocyanin (C-PC) phycobiliprotein from Spirulina microalgae. For this purpose, we incorporated C-PC in combined starch and β-lactoglobulin (BLG) hydrogels by using a high-pressure (HP) process. Indeed, in thermal treatment, the color derived from C-PC is entirely lost. We characterized the obtained HP gels by both rheology and small-angle X-ray scattering (SAXS). Various formulations of binary (BLG/C-PC) and ternary (starch/BLG/C-PC) systems were tested under HP up to 4,500 bar. A good preservation of the C-PC pigment was established by mixing BLG and starch with C-PC at pH 7, with concentrations of 180, 5, and 10 mg/mL, respectively. Identical component concentrations were maintained in the binary systems. Structure of gels was characterized by SAXS providing insight of C-PC interactions with BLG and starch after HP process which leads to the formation of solid gels with larger mesh compared to two-component systems. This results in enhanced mechanical properties, which were determined by amplitude and frequency sweep measurements using a rheometer with applied plane/plane geometry. Therefore, adding starch, even at small concentration, significantly improves gel visual appearance and mechanical properties. Our study reveals that preservation through HP treatment is more effective than high temperature treatment, as visually observed through the sustained color integrity of C-PC blue dye. PB - Serbian Biochemical Society C3 - Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia T1 - Combined hydrogels of starch and β-lactoglobulin as matrices for the preservation of C-phycocyanin SP - 150 EP - 150 UR - https://hdl.handle.net/21.15107/rcub_cer_6669 ER -
@conference{ author = "Jovanović, Zorana and Annighöfer, Burkhard and Dudzinski, Daniel and Veličković, Luka and Gligorijević, Nikola and Nikolić, Milan and Brûlet, Annie and Assifaoui, Ali and Combet, Sophie and Minić, Simeon", year = "2023", abstract = "The color of food products is an important aspect in food industry, and its preservation remains a big challenge. We aim to preserve the natural blue dye of C-phycocyanin (C-PC) phycobiliprotein from Spirulina microalgae. For this purpose, we incorporated C-PC in combined starch and β-lactoglobulin (BLG) hydrogels by using a high-pressure (HP) process. Indeed, in thermal treatment, the color derived from C-PC is entirely lost. We characterized the obtained HP gels by both rheology and small-angle X-ray scattering (SAXS). Various formulations of binary (BLG/C-PC) and ternary (starch/BLG/C-PC) systems were tested under HP up to 4,500 bar. A good preservation of the C-PC pigment was established by mixing BLG and starch with C-PC at pH 7, with concentrations of 180, 5, and 10 mg/mL, respectively. Identical component concentrations were maintained in the binary systems. Structure of gels was characterized by SAXS providing insight of C-PC interactions with BLG and starch after HP process which leads to the formation of solid gels with larger mesh compared to two-component systems. This results in enhanced mechanical properties, which were determined by amplitude and frequency sweep measurements using a rheometer with applied plane/plane geometry. Therefore, adding starch, even at small concentration, significantly improves gel visual appearance and mechanical properties. Our study reveals that preservation through HP treatment is more effective than high temperature treatment, as visually observed through the sustained color integrity of C-PC blue dye.", publisher = "Serbian Biochemical Society", journal = "Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia", title = "Combined hydrogels of starch and β-lactoglobulin as matrices for the preservation of C-phycocyanin", pages = "150-150", url = "https://hdl.handle.net/21.15107/rcub_cer_6669" }
Jovanović, Z., Annighöfer, B., Dudzinski, D., Veličković, L., Gligorijević, N., Nikolić, M., Brûlet, A., Assifaoui, A., Combet, S.,& Minić, S.. (2023). Combined hydrogels of starch and β-lactoglobulin as matrices for the preservation of C-phycocyanin. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia Serbian Biochemical Society., 150-150. https://hdl.handle.net/21.15107/rcub_cer_6669
Jovanović Z, Annighöfer B, Dudzinski D, Veličković L, Gligorijević N, Nikolić M, Brûlet A, Assifaoui A, Combet S, Minić S. Combined hydrogels of starch and β-lactoglobulin as matrices for the preservation of C-phycocyanin. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia. 2023;:150-150. https://hdl.handle.net/21.15107/rcub_cer_6669 .
Jovanović, Zorana, Annighöfer, Burkhard, Dudzinski, Daniel, Veličković, Luka, Gligorijević, Nikola, Nikolić, Milan, Brûlet, Annie, Assifaoui, Ali, Combet, Sophie, Minić, Simeon, "Combined hydrogels of starch and β-lactoglobulin as matrices for the preservation of C-phycocyanin" in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia (2023):150-150, https://hdl.handle.net/21.15107/rcub_cer_6669 .