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Crystallographic structure and molecular dynamics simulations of the major endoglucanase from Xanthomonas campestris pv. campestris shed light on its oligosaccharide products release pattern
dc.creator | Puhl, Ana C. | |
dc.creator | Prates, Erica T. | |
dc.creator | Rosseto, Flávio R. | |
dc.creator | Manzine, Livia R. | |
dc.creator | Stanković, Ivana | |
dc.creator | de Araújo, Simara S. | |
dc.creator | Alvarez, Thabata M. | |
dc.creator | Squina, Fábio M. | |
dc.creator | Skaf, Munir S. | |
dc.creator | Polikarpov, Igor | |
dc.date.accessioned | 2023-05-06T16:30:12Z | |
dc.date.available | 2023-05-06T16:30:12Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0141-8130 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/6083 | |
dc.description.abstract | Cellulases are essential enzymatic components for the transformation of plant biomass into fuels, renewable ma- terials and green chemicals. Here, we determined the crystal structure, pattern of hydrolysis products release, and conducted molecular dynamics simulations of the major endoglucanase from the Xanthomonas campestris pv. campestris (XccCel5A). XccCel5A has a TIM barrel fold with the catalytic site centrally placed in a binding groove surrounded by aromatic side chains. Molecular dynamics simulations show that productive position of the substrate is secured by a network of hydrogen bonds in the four main subsites, which differ in details from homologous structures. Capillary zone electrophoresis and computational studies reveal XccCel5A can act both as endoglucanase and licheninase, but there are preferable arrangements of substrate regarding β-1,3 and β- 1,4 bonds within the binding cleft which are related to the enzymatic efficiency. | sr |
dc.language.iso | en | sr |
dc.publisher | Elsevier B.V. | sr |
dc.relation | Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) grant 10/52362-5 | sr |
dc.relation | Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) grant 11/20505-4 | sr |
dc.relation | Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) grant 11/ 21608-1 | sr |
dc.relation | Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) grant 15/50590-4 | sr |
dc.relation | Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) grant 15/13684-0 | sr |
dc.relation | INCT Bioetanol (FAPESP/CNPq) | sr |
dc.relation | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grant 405191/2015-4 | sr |
dc.relation | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grant 303988/2016-9 | sr |
dc.relation | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grant 440977/2016-9 | sr |
dc.relation | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grant 151963/2018-5 | sr |
dc.relation | MCT/CNPq/FAPESP EU-Brazil Collaboration program in Second Generation Biofuels (CeProBio Project; FAPESP 2009/52840-7 and CNPq 490022/2009-0) | sr |
dc.rights | restrictedAccess | sr |
dc.source | International Journal of Biological Macromolecules | sr |
dc.subject | Endoglucanase | sr |
dc.subject | Molecular dynamics | sr |
dc.subject | X-ray structure | sr |
dc.title | Crystallographic structure and molecular dynamics simulations of the major endoglucanase from Xanthomonas campestris pv. campestris shed light on its oligosaccharide products release pattern | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.citation.volume | 136 | |
dc.citation.spage | 493 | |
dc.citation.epage | 502 | |
dc.citation.rank | aM21 | |
dc.identifier.pmid | 0141-8130 | |
dc.identifier.doi | 10.1016/j.ijbiomac.2019.06.107 | |
dc.identifier.scopus | 2-s2.0-85067429708 | |
dc.type.version | publishedVersion | sr |