Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources
Аутори
Stojanović, SanjaStepanović, Jelena
Ristović, Marina
Dojnov, Biljana
Božić, Nataša
Duduk, Bojan
Vujčić, Zoran
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Fungal inulinases have wide application in industrial biotechnology, and it is presumed that their expression is regulated at the transcriptional level via promoter. It is also known that different sugars have an inducing effect on gene expression in fungal genome, including inulinases. Aim of this work was to determine which of the sugars used in growth medium, as the only carbon source, induce the extracellular exoinulinase gene inuE expression in Aspergillus welwitschiae FAW1. Inulin, rafinose, sucrose, glucose and fructose were used as carbon sources, and expression of inuE was monitored during 72 h of cultivation (tested after 24, 36, 48 and 72 h). Both, presence of mRNA in the mycelia and extracellular enzyme activity in the growth media were monitored. Interestingly,
obtained results showed that inuE was induced by fructose, sucrose and rafinose and not by inulin. In all cases, the highest mRNA was detected after 24 h of cultivation, while extracellular exoinulinase activity in...creased from 24 h with a peak in 72 h. Further experiments are necessary for a comprehensive understanding of the regulation mechanisms of AweinuE promoter for its more purposeful application in biotechnology.
Кључне речи:
inulinases / Aspergillus welwitschiae / carbon source / exoinulinaseИзвор:
Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022, 2022, 141-Издавач:
- University of Belgrade - Faculty of Chemistry
- Serbian Biochemical Society
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-200168)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200214 (Институт за пестициде и заштиту животне средине, Београд) (RS-200214)
Институција/група
IHTMTY - CONF AU - Stojanović, Sanja AU - Stepanović, Jelena AU - Ristović, Marina AU - Dojnov, Biljana AU - Božić, Nataša AU - Duduk, Bojan AU - Vujčić, Zoran PY - 2022 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5920 AB - Fungal inulinases have wide application in industrial biotechnology, and it is presumed that their expression is regulated at the transcriptional level via promoter. It is also known that different sugars have an inducing effect on gene expression in fungal genome, including inulinases. Aim of this work was to determine which of the sugars used in growth medium, as the only carbon source, induce the extracellular exoinulinase gene inuE expression in Aspergillus welwitschiae FAW1. Inulin, rafinose, sucrose, glucose and fructose were used as carbon sources, and expression of inuE was monitored during 72 h of cultivation (tested after 24, 36, 48 and 72 h). Both, presence of mRNA in the mycelia and extracellular enzyme activity in the growth media were monitored. Interestingly, obtained results showed that inuE was induced by fructose, sucrose and rafinose and not by inulin. In all cases, the highest mRNA was detected after 24 h of cultivation, while extracellular exoinulinase activity increased from 24 h with a peak in 72 h. Further experiments are necessary for a comprehensive understanding of the regulation mechanisms of AweinuE promoter for its more purposeful application in biotechnology. PB - University of Belgrade - Faculty of Chemistry PB - Serbian Biochemical Society C3 - Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022 T1 - Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources SP - 141 UR - https://hdl.handle.net/21.15107/rcub_cer_5920 ER -
@conference{ author = "Stojanović, Sanja and Stepanović, Jelena and Ristović, Marina and Dojnov, Biljana and Božić, Nataša and Duduk, Bojan and Vujčić, Zoran", year = "2022", abstract = "Fungal inulinases have wide application in industrial biotechnology, and it is presumed that their expression is regulated at the transcriptional level via promoter. It is also known that different sugars have an inducing effect on gene expression in fungal genome, including inulinases. Aim of this work was to determine which of the sugars used in growth medium, as the only carbon source, induce the extracellular exoinulinase gene inuE expression in Aspergillus welwitschiae FAW1. Inulin, rafinose, sucrose, glucose and fructose were used as carbon sources, and expression of inuE was monitored during 72 h of cultivation (tested after 24, 36, 48 and 72 h). Both, presence of mRNA in the mycelia and extracellular enzyme activity in the growth media were monitored. Interestingly, obtained results showed that inuE was induced by fructose, sucrose and rafinose and not by inulin. In all cases, the highest mRNA was detected after 24 h of cultivation, while extracellular exoinulinase activity increased from 24 h with a peak in 72 h. Further experiments are necessary for a comprehensive understanding of the regulation mechanisms of AweinuE promoter for its more purposeful application in biotechnology.", publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society", journal = "Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022", title = "Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources", pages = "141", url = "https://hdl.handle.net/21.15107/rcub_cer_5920" }
Stojanović, S., Stepanović, J., Ristović, M., Dojnov, B., Božić, N., Duduk, B.,& Vujčić, Z.. (2022). Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022 University of Belgrade - Faculty of Chemistry., 141. https://hdl.handle.net/21.15107/rcub_cer_5920
Stojanović S, Stepanović J, Ristović M, Dojnov B, Božić N, Duduk B, Vujčić Z. Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022. 2022;:141. https://hdl.handle.net/21.15107/rcub_cer_5920 .
Stojanović, Sanja, Stepanović, Jelena, Ristović, Marina, Dojnov, Biljana, Božić, Nataša, Duduk, Bojan, Vujčić, Zoran, "Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources" in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022 (2022):141, https://hdl.handle.net/21.15107/rcub_cer_5920 .