Selectivity issues in affinity-based biochemical sensors: Determining the ratio of similar biomolecules in binary mixtures
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We consider selectivity of affinity-based nanosensors utilizing resonance shift due to the presence of adsorbed analyte. Among such devices we analyze mass-based sensors utilizing mechanical resonance in e.g. micro or
nanocantilevers and all-optical refractometric sensors utilizing surface plasmon polariton resonance. The sensitivity of such devices can be extremely high, reaching single-molecule level, however their selectivity is limited by the differences in mass in the first case or in refractive index values between different analytes in the second. The typical approach is to use some kind of receptors on the sensor surface with highly specific binding of a targeted analyte. The properties of a given biomolecule, for instance protein, will vary between its different conformations due to different arrangements of their atoms in space. Since the conformation of a molecule is critically important for its
function, it is of interest to determine the ratio between different conformat...ional isomers in a given sample. In general, different conformations of a biomolecule may have different affinity toward binding sites on the surface of an affinity-based nanosensor, as well as different surface-volume ratios. We argue
that the analysis of adsorption kinetics ensures sufficient data to discriminate between different conformations in a mixture.
Ključne reči:
Biochemical sensors / Affinity Sensors / Microcantilevers / Surface Plasmon Polaritons / Refractometric Devices / Adsorption Kinetics / Desorption / Sensor SelectivityIzvor:
Proceedings - 1st International Conference on Electrical, Electronic and Computing Engineering IcETRAN 2014, 2014, MOI2.5.1-MOI2.5.4Izdavač:
- Beograd : Društvo za ETRAN / Belgrade : ETRAN Society
Finansiranje / projekti:
- Mikro, nano-sistemi i senzori za primenu u elektroprivredi, procesnoj industriji i zaštiti životne sredine (RS-MESTD-Technological Development (TD or TR)-32008)
Institucija/grupa
IHTMTY - CONF AU - Jokić, Ivana AU - Jakšić, Olga AU - Jakšić, Zoran AU - Radulović, Katarina AU - Frantlović, Miloš PY - 2014 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5734 AB - We consider selectivity of affinity-based nanosensors utilizing resonance shift due to the presence of adsorbed analyte. Among such devices we analyze mass-based sensors utilizing mechanical resonance in e.g. micro or nanocantilevers and all-optical refractometric sensors utilizing surface plasmon polariton resonance. The sensitivity of such devices can be extremely high, reaching single-molecule level, however their selectivity is limited by the differences in mass in the first case or in refractive index values between different analytes in the second. The typical approach is to use some kind of receptors on the sensor surface with highly specific binding of a targeted analyte. The properties of a given biomolecule, for instance protein, will vary between its different conformations due to different arrangements of their atoms in space. Since the conformation of a molecule is critically important for its function, it is of interest to determine the ratio between different conformational isomers in a given sample. In general, different conformations of a biomolecule may have different affinity toward binding sites on the surface of an affinity-based nanosensor, as well as different surface-volume ratios. We argue that the analysis of adsorption kinetics ensures sufficient data to discriminate between different conformations in a mixture. PB - Beograd : Društvo za ETRAN / Belgrade : ETRAN Society C3 - Proceedings - 1st International Conference on Electrical, Electronic and Computing Engineering IcETRAN 2014 T1 - Selectivity issues in affinity-based biochemical sensors: Determining the ratio of similar biomolecules in binary mixtures SP - MOI2.5.1 EP - MOI2.5.4 UR - https://hdl.handle.net/21.15107/rcub_cer_5734 ER -
@conference{ author = "Jokić, Ivana and Jakšić, Olga and Jakšić, Zoran and Radulović, Katarina and Frantlović, Miloš", year = "2014", abstract = "We consider selectivity of affinity-based nanosensors utilizing resonance shift due to the presence of adsorbed analyte. Among such devices we analyze mass-based sensors utilizing mechanical resonance in e.g. micro or nanocantilevers and all-optical refractometric sensors utilizing surface plasmon polariton resonance. The sensitivity of such devices can be extremely high, reaching single-molecule level, however their selectivity is limited by the differences in mass in the first case or in refractive index values between different analytes in the second. The typical approach is to use some kind of receptors on the sensor surface with highly specific binding of a targeted analyte. The properties of a given biomolecule, for instance protein, will vary between its different conformations due to different arrangements of their atoms in space. Since the conformation of a molecule is critically important for its function, it is of interest to determine the ratio between different conformational isomers in a given sample. In general, different conformations of a biomolecule may have different affinity toward binding sites on the surface of an affinity-based nanosensor, as well as different surface-volume ratios. We argue that the analysis of adsorption kinetics ensures sufficient data to discriminate between different conformations in a mixture.", publisher = "Beograd : Društvo za ETRAN / Belgrade : ETRAN Society", journal = "Proceedings - 1st International Conference on Electrical, Electronic and Computing Engineering IcETRAN 2014", title = "Selectivity issues in affinity-based biochemical sensors: Determining the ratio of similar biomolecules in binary mixtures", pages = "MOI2.5.1-MOI2.5.4", url = "https://hdl.handle.net/21.15107/rcub_cer_5734" }
Jokić, I., Jakšić, O., Jakšić, Z., Radulović, K.,& Frantlović, M.. (2014). Selectivity issues in affinity-based biochemical sensors: Determining the ratio of similar biomolecules in binary mixtures. in Proceedings - 1st International Conference on Electrical, Electronic and Computing Engineering IcETRAN 2014 Beograd : Društvo za ETRAN / Belgrade : ETRAN Society., MOI2.5.1-MOI2.5.4. https://hdl.handle.net/21.15107/rcub_cer_5734
Jokić I, Jakšić O, Jakšić Z, Radulović K, Frantlović M. Selectivity issues in affinity-based biochemical sensors: Determining the ratio of similar biomolecules in binary mixtures. in Proceedings - 1st International Conference on Electrical, Electronic and Computing Engineering IcETRAN 2014. 2014;:MOI2.5.1-MOI2.5.4. https://hdl.handle.net/21.15107/rcub_cer_5734 .
Jokić, Ivana, Jakšić, Olga, Jakšić, Zoran, Radulović, Katarina, Frantlović, Miloš, "Selectivity issues in affinity-based biochemical sensors: Determining the ratio of similar biomolecules in binary mixtures" in Proceedings - 1st International Conference on Electrical, Electronic and Computing Engineering IcETRAN 2014 (2014):MOI2.5.1-MOI2.5.4, https://hdl.handle.net/21.15107/rcub_cer_5734 .