Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae
Samo za registrovane korisnike
2022
Autori
Gođevac, DejanIvanović, Stefan
Simić, Katarina
Anđelković, Boban
Jovanović, Živko
Rakić, Tamara
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Introduction
Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated.
Objective
To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process
Methods
Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates.
Results
Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved ...in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples.
Conclusion
Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.
Ključne reči:
Ramonda / resurrection plant / metabolomics / NMR / GC–MSIzvor:
Phytochemical Analysis, 2022, 33, 6, 961-970Izdavač:
- Willey
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200026 (Univerzitet u Beogradu, Institut za hemiju, tehnologiju i metalurgiju - IHTM) (RS-MESTD-inst-2020-200026)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-MESTD-inst-2020-200178)
DOI: 10.1002/pca.3151
ISSN: 0958-0344
WoS: 00081078340000
Scopus: 2-s2.0-85131833166
Institucija/grupa
IHTMTY - JOUR AU - Gođevac, Dejan AU - Ivanović, Stefan AU - Simić, Katarina AU - Anđelković, Boban AU - Jovanović, Živko AU - Rakić, Tamara PY - 2022 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5165 AB - Introduction Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated. Objective To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process Methods Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates. Results Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples. Conclusion Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants. PB - Willey T2 - Phytochemical Analysis T1 - Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae VL - 33 IS - 6 SP - 961 SP - 961 EP - 970 DO - 10.1002/pca.3151 ER -
@article{ author = "Gođevac, Dejan and Ivanović, Stefan and Simić, Katarina and Anđelković, Boban and Jovanović, Živko and Rakić, Tamara", year = "2022", abstract = "Introduction Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated. Objective To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process Methods Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates. Results Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples. Conclusion Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.", publisher = "Willey", journal = "Phytochemical Analysis", title = "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae", volume = "33", number = "6", pages = "961-961-970", doi = "10.1002/pca.3151" }
Gođevac, D., Ivanović, S., Simić, K., Anđelković, B., Jovanović, Ž.,& Rakić, T.. (2022). Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis Willey., 33(6), 961-970. https://doi.org/10.1002/pca.3151
Gođevac D, Ivanović S, Simić K, Anđelković B, Jovanović Ž, Rakić T. Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis. 2022;33(6):961-970. doi:10.1002/pca.3151 .
Gođevac, Dejan, Ivanović, Stefan, Simić, Katarina, Anđelković, Boban, Jovanović, Živko, Rakić, Tamara, "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae" in Phytochemical Analysis, 33, no. 6 (2022):961-970, https://doi.org/10.1002/pca.3151 . .