Study on the assessment of humification processes during biodegradation of heavy residual fuel oil
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2021
Authors
Avdalović, JelenaMiletić, Srđan
Božović, Olga
Šolević Knudsen, Tatjana
Stanković, Dalibor
Lugonja, Nikoleta
Spasić, Snežana
Joksimović, Kristina
Dragičević, Igor
Vrvić, Miroslav
Article (Published version)
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The aim of this study was to investigate the creation of humic substances during biodegradation of heavy residual fuel oil, because there are indications that substances similar to humic substances are generated during biodegradation of polycyclic aromatic hydrocarbons. In the study, which lasted for 110 days, biodegradation of heavy residual fuel oil was carried out in a layer of artificial soil substrate. The initial concentration of the total petroleum hydrocarbon in the prepared artificial soil substrate (biopile) was 23.1 g kg-1 dry weight (d.w.). At the end of the process, the total petroleum hydrocarbons were reduced to 8.1 g kg-1 d.w. in the inoculated biopile, while the content of humic acids increased during bioremediation from 3.15 g kg-1 d.w. to 4.95 g kg-1 d.w. The humic acids extracted from biopile during the biodegradation process were characterized by various chemical techniques (elemental analysis, spectrofluorimetric analysis, electrochemical measurements, and size ex...clusion chromatography). The results showed that levels of C, H and the H/C ratio decreased as the biodegradation process progressed. This indicated that humic acids aromatization process took place and this was confirmed by the spectrofluorimetric analysis. The increase of oxygen percentage and the O/C ratio in the humic acids after the biodegradation treatment indicated an increase in functional oxygen groups. Additional analyses of humic acids from the inoculated biopile showed that they were transformed during the bioremediation process. They had greater redox and buffering capacities and a larger portion of the fractions had high molecular mass. Also, the humification parameters (the CHAs/CFAs ratio and CHAs/Corg ratio) increased during the biodegradation. This is one of the few studies that describes the generation of humic substances during the biodegradation of oil compounds.
Keywords:
Humic acids / Humification parameters / Zymogenic microorganisms / Biodegradation / Heavy residual fuel oilSource:
Science of the Total Environment, 2021, 797, 149099-Publisher:
- Elsevier
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-MESTD-inst-2020-200026)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200168 (University of Belgrade, Faculty of Chemistry) (RS-MESTD-inst-2020-200168)
DOI: 10.1016/j.scitotenv.2021.149099
ISSN: 0048-9697
PubMed: 34303984
WoS: 000704363800004
Scopus: 2-s2.0-85111806517
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IHTMTY - JOUR AU - Avdalović, Jelena AU - Miletić, Srđan AU - Božović, Olga AU - Šolević Knudsen, Tatjana AU - Stanković, Dalibor AU - Lugonja, Nikoleta AU - Spasić, Snežana AU - Joksimović, Kristina AU - Dragičević, Igor AU - Vrvić, Miroslav PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4753 AB - The aim of this study was to investigate the creation of humic substances during biodegradation of heavy residual fuel oil, because there are indications that substances similar to humic substances are generated during biodegradation of polycyclic aromatic hydrocarbons. In the study, which lasted for 110 days, biodegradation of heavy residual fuel oil was carried out in a layer of artificial soil substrate. The initial concentration of the total petroleum hydrocarbon in the prepared artificial soil substrate (biopile) was 23.1 g kg-1 dry weight (d.w.). At the end of the process, the total petroleum hydrocarbons were reduced to 8.1 g kg-1 d.w. in the inoculated biopile, while the content of humic acids increased during bioremediation from 3.15 g kg-1 d.w. to 4.95 g kg-1 d.w. The humic acids extracted from biopile during the biodegradation process were characterized by various chemical techniques (elemental analysis, spectrofluorimetric analysis, electrochemical measurements, and size exclusion chromatography). The results showed that levels of C, H and the H/C ratio decreased as the biodegradation process progressed. This indicated that humic acids aromatization process took place and this was confirmed by the spectrofluorimetric analysis. The increase of oxygen percentage and the O/C ratio in the humic acids after the biodegradation treatment indicated an increase in functional oxygen groups. Additional analyses of humic acids from the inoculated biopile showed that they were transformed during the bioremediation process. They had greater redox and buffering capacities and a larger portion of the fractions had high molecular mass. Also, the humification parameters (the CHAs/CFAs ratio and CHAs/Corg ratio) increased during the biodegradation. This is one of the few studies that describes the generation of humic substances during the biodegradation of oil compounds. PB - Elsevier T2 - Science of the Total Environment T1 - Study on the assessment of humification processes during biodegradation of heavy residual fuel oil VL - 797 SP - 149099 DO - 10.1016/j.scitotenv.2021.149099 ER -
@article{ author = "Avdalović, Jelena and Miletić, Srđan and Božović, Olga and Šolević Knudsen, Tatjana and Stanković, Dalibor and Lugonja, Nikoleta and Spasić, Snežana and Joksimović, Kristina and Dragičević, Igor and Vrvić, Miroslav", year = "2021", abstract = "The aim of this study was to investigate the creation of humic substances during biodegradation of heavy residual fuel oil, because there are indications that substances similar to humic substances are generated during biodegradation of polycyclic aromatic hydrocarbons. In the study, which lasted for 110 days, biodegradation of heavy residual fuel oil was carried out in a layer of artificial soil substrate. The initial concentration of the total petroleum hydrocarbon in the prepared artificial soil substrate (biopile) was 23.1 g kg-1 dry weight (d.w.). At the end of the process, the total petroleum hydrocarbons were reduced to 8.1 g kg-1 d.w. in the inoculated biopile, while the content of humic acids increased during bioremediation from 3.15 g kg-1 d.w. to 4.95 g kg-1 d.w. The humic acids extracted from biopile during the biodegradation process were characterized by various chemical techniques (elemental analysis, spectrofluorimetric analysis, electrochemical measurements, and size exclusion chromatography). The results showed that levels of C, H and the H/C ratio decreased as the biodegradation process progressed. This indicated that humic acids aromatization process took place and this was confirmed by the spectrofluorimetric analysis. The increase of oxygen percentage and the O/C ratio in the humic acids after the biodegradation treatment indicated an increase in functional oxygen groups. Additional analyses of humic acids from the inoculated biopile showed that they were transformed during the bioremediation process. They had greater redox and buffering capacities and a larger portion of the fractions had high molecular mass. Also, the humification parameters (the CHAs/CFAs ratio and CHAs/Corg ratio) increased during the biodegradation. This is one of the few studies that describes the generation of humic substances during the biodegradation of oil compounds.", publisher = "Elsevier", journal = "Science of the Total Environment", title = "Study on the assessment of humification processes during biodegradation of heavy residual fuel oil", volume = "797", pages = "149099", doi = "10.1016/j.scitotenv.2021.149099" }
Avdalović, J., Miletić, S., Božović, O., Šolević Knudsen, T., Stanković, D., Lugonja, N., Spasić, S., Joksimović, K., Dragičević, I.,& Vrvić, M.. (2021). Study on the assessment of humification processes during biodegradation of heavy residual fuel oil. in Science of the Total Environment Elsevier., 797, 149099. https://doi.org/10.1016/j.scitotenv.2021.149099
Avdalović J, Miletić S, Božović O, Šolević Knudsen T, Stanković D, Lugonja N, Spasić S, Joksimović K, Dragičević I, Vrvić M. Study on the assessment of humification processes during biodegradation of heavy residual fuel oil. in Science of the Total Environment. 2021;797:149099. doi:10.1016/j.scitotenv.2021.149099 .
Avdalović, Jelena, Miletić, Srđan, Božović, Olga, Šolević Knudsen, Tatjana, Stanković, Dalibor, Lugonja, Nikoleta, Spasić, Snežana, Joksimović, Kristina, Dragičević, Igor, Vrvić, Miroslav, "Study on the assessment of humification processes during biodegradation of heavy residual fuel oil" in Science of the Total Environment, 797 (2021):149099, https://doi.org/10.1016/j.scitotenv.2021.149099 . .