Green frogs skin harbours microbiota treasure
Samo za registrovane korisnike
2019
Autori
Krizmanić, JelenaStupar, Miloš
Ljaljević Grbić, Milica
Dimitrijević, Jelica
Vidaković, Danijela
Stamenković, Srđan
Krizmanić, Imre
Breka, Katarina
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Cutaneous microbiota of amphibians is considered
to be an indicator of the health status of examined
individuals, as well as of environmental factors. In
that sense the skin of the captured individuals
belonging to Pelophylax esculenta complex on the
locality Stevanove ravnice within the Special Nature
Reserve “Deliblato Sand” (Serbia) was screened for
the presence of biofilm-forming cyanobacteria,
algae and fungi dwelling directly on frogs’ stratum
corneum. Biofilm was sampled using non-aggressive
“adhesive tape method” and their constituents were
analyzed and characterized microscopically (Carl
Zeiss AxioImager.M1 with AxioVision 4.9 software).
We detected high diversity and abundance
of diatom taxa overall, particularly belonging to
genera Navicula, Gomphonema, Geissleria,
Placoneis and Nitzschia. Cyanobacteria were less
present and with low abundance. Among them
chroococcal genera Gloeocapsopsis, Pseudocapsa
and Aphanocapsa were recorded. Outside of diatoms
and ...cyanobacteria, genus Chlorella was the
only present photoautotrophic biofilm constituent.
We consider highly mucous green frogs skin as
a favorable environment for the survival of diatoms
and cyanobacteria. Light microscopy analyses of
biofilm fragments revealed the presence of variety
of fungal structures. Documented coenocytic mycelium
with zoosporangium, originated presumably
from oomycetes, as well as septate melanized
hyphae of dematiaceous fungi, suggested colonization
of frog skin from both types of environments
present, water and surrounding vegetation.
A variety of fungal spores (fragmospores, amerospores,
dictyospores, didymospores), and in few
cases spore germination were recorded on frog
skin. Detected biofilm composition was not determinate
by species and gender-specific.
Ključne reči:
amphibias / biofilm / environment / ecology / green frogIzvor:
European Journal of Phycology (Posters), 2019, 54, sup1, 156-156Izdavač:
- Taylor & Francis
Napomena:
- 7th European Phycological Congress, 25-30 August 2019, Zagreb
Institucija/grupa
IHTMTY - CONF AU - Krizmanić, Jelena AU - Stupar, Miloš AU - Ljaljević Grbić, Milica AU - Dimitrijević, Jelica AU - Vidaković, Danijela AU - Stamenković, Srđan AU - Krizmanić, Imre AU - Breka, Katarina PY - 2019 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4622 AB - Cutaneous microbiota of amphibians is considered to be an indicator of the health status of examined individuals, as well as of environmental factors. In that sense the skin of the captured individuals belonging to Pelophylax esculenta complex on the locality Stevanove ravnice within the Special Nature Reserve “Deliblato Sand” (Serbia) was screened for the presence of biofilm-forming cyanobacteria, algae and fungi dwelling directly on frogs’ stratum corneum. Biofilm was sampled using non-aggressive “adhesive tape method” and their constituents were analyzed and characterized microscopically (Carl Zeiss AxioImager.M1 with AxioVision 4.9 software). We detected high diversity and abundance of diatom taxa overall, particularly belonging to genera Navicula, Gomphonema, Geissleria, Placoneis and Nitzschia. Cyanobacteria were less present and with low abundance. Among them chroococcal genera Gloeocapsopsis, Pseudocapsa and Aphanocapsa were recorded. Outside of diatoms and cyanobacteria, genus Chlorella was the only present photoautotrophic biofilm constituent. We consider highly mucous green frogs skin as a favorable environment for the survival of diatoms and cyanobacteria. Light microscopy analyses of biofilm fragments revealed the presence of variety of fungal structures. Documented coenocytic mycelium with zoosporangium, originated presumably from oomycetes, as well as septate melanized hyphae of dematiaceous fungi, suggested colonization of frog skin from both types of environments present, water and surrounding vegetation. A variety of fungal spores (fragmospores, amerospores, dictyospores, didymospores), and in few cases spore germination were recorded on frog skin. Detected biofilm composition was not determinate by species and gender-specific. PB - Taylor & Francis C3 - European Journal of Phycology (Posters) T1 - Green frogs skin harbours microbiota treasure VL - 54 IS - sup1 SP - 156 EP - 156 DO - 10.1080/09670262.2019.1626628 ER -
@conference{ author = "Krizmanić, Jelena and Stupar, Miloš and Ljaljević Grbić, Milica and Dimitrijević, Jelica and Vidaković, Danijela and Stamenković, Srđan and Krizmanić, Imre and Breka, Katarina", year = "2019", abstract = "Cutaneous microbiota of amphibians is considered to be an indicator of the health status of examined individuals, as well as of environmental factors. In that sense the skin of the captured individuals belonging to Pelophylax esculenta complex on the locality Stevanove ravnice within the Special Nature Reserve “Deliblato Sand” (Serbia) was screened for the presence of biofilm-forming cyanobacteria, algae and fungi dwelling directly on frogs’ stratum corneum. Biofilm was sampled using non-aggressive “adhesive tape method” and their constituents were analyzed and characterized microscopically (Carl Zeiss AxioImager.M1 with AxioVision 4.9 software). We detected high diversity and abundance of diatom taxa overall, particularly belonging to genera Navicula, Gomphonema, Geissleria, Placoneis and Nitzschia. Cyanobacteria were less present and with low abundance. Among them chroococcal genera Gloeocapsopsis, Pseudocapsa and Aphanocapsa were recorded. Outside of diatoms and cyanobacteria, genus Chlorella was the only present photoautotrophic biofilm constituent. We consider highly mucous green frogs skin as a favorable environment for the survival of diatoms and cyanobacteria. Light microscopy analyses of biofilm fragments revealed the presence of variety of fungal structures. Documented coenocytic mycelium with zoosporangium, originated presumably from oomycetes, as well as septate melanized hyphae of dematiaceous fungi, suggested colonization of frog skin from both types of environments present, water and surrounding vegetation. A variety of fungal spores (fragmospores, amerospores, dictyospores, didymospores), and in few cases spore germination were recorded on frog skin. Detected biofilm composition was not determinate by species and gender-specific.", publisher = "Taylor & Francis", journal = "European Journal of Phycology (Posters)", title = "Green frogs skin harbours microbiota treasure", volume = "54", number = "sup1", pages = "156-156", doi = "10.1080/09670262.2019.1626628" }
Krizmanić, J., Stupar, M., Ljaljević Grbić, M., Dimitrijević, J., Vidaković, D., Stamenković, S., Krizmanić, I.,& Breka, K.. (2019). Green frogs skin harbours microbiota treasure. in European Journal of Phycology (Posters) Taylor & Francis., 54(sup1), 156-156. https://doi.org/10.1080/09670262.2019.1626628
Krizmanić J, Stupar M, Ljaljević Grbić M, Dimitrijević J, Vidaković D, Stamenković S, Krizmanić I, Breka K. Green frogs skin harbours microbiota treasure. in European Journal of Phycology (Posters). 2019;54(sup1):156-156. doi:10.1080/09670262.2019.1626628 .
Krizmanić, Jelena, Stupar, Miloš, Ljaljević Grbić, Milica, Dimitrijević, Jelica, Vidaković, Danijela, Stamenković, Srđan, Krizmanić, Imre, Breka, Katarina, "Green frogs skin harbours microbiota treasure" in European Journal of Phycology (Posters), 54, no. sup1 (2019):156-156, https://doi.org/10.1080/09670262.2019.1626628 . .