Prikaz osnovnih podataka o dokumentu
Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation
dc.creator | Perovic, I. | |
dc.creator | Milovanovic, M. | |
dc.creator | Stanić, Dragana | |
dc.creator | Burazer, Lidija M. | |
dc.creator | Petrović, D. | |
dc.creator | Milčić-Matić, Natalija | |
dc.creator | Gafvelin, Guro | |
dc.creator | van Hage, Marianne | |
dc.creator | Jankov, Ratko M. | |
dc.creator | Ćirković-Veličković, Tanja | |
dc.date.accessioned | 2021-03-07T23:20:35Z | |
dc.date.available | 2021-03-07T23:20:35Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0954-7894 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/4324 | |
dc.description.abstract | Treating allergies with modified allergens is an approach to make the treatment safer and more efficient. Art v 1 is the most prominent allergen of mugwort pollen and a significant cause of hayfever around Europe. The aim of this study was to reduce the allergenicity of Art v 1 by acetylation, and to investigate the capacity of the modified protein to generate blocking antibodies. The reduction of allergenicity of Art v 1 following acetylation was monitored by immunoblot, ELISA inhibition using a pool of sera from mugwort pollen allergic patients, basophil activation assay and by skin prick testing of mugwort-allergic patients. Rabbits were immunized against Art v 1 and acetylated Art v 1 (acArt v 1) and the rabbit antisera were tested for their capacity to block human IgE binding in ELISA. Human T cell proliferation against Art v 1 and acArt v 1 was examined in peripheral blood mononuclear cells (PBMCs) of mugwort pollen allergic patients and cytokine release in PBMC cultures was monitored. Acetylation of Art v 1 gave a derivative of reduced allergenicity in the in vitro and ex vivo tests applied. The skin test reactivity to acArt v 1 was significantly reduced in 19 patients when compared with the reactivity to Art v 1. Rabbit antibodies to acArt v 1 and Art v 1 showed similar capacity to block human IgE binding to Art v 1 in inhibition ELISA. Both proteins were able to induce proliferation of PBMCs and CD3/CD4(+) cells of mugwort-allergic patients. Release of IL-5 was significantly reduced in cultures stimulated with acArt v 1. Art v 1 modified by acetylation had a significantly reduced allergenicity in vitro and in vivo, while its immunogenicity was retained. Modification of allergens by acetylation could be a new strategy for allergen-specific immunotherapy. Cite this as: I. Perovic, M. Milovanovic, D. Stanic, L. Burazer, D. Petrovic, N. Milcic-Matic, G. Gafvelin, M. van Hage, R. Jankov and T. Cirkovic Velickovic, Clinical and Experimental Allergy, 2009 (39) 435-446. | en |
dc.publisher | Wiley-Blackwell Publishing, Inc, Malden | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS// | |
dc.rights | restrictedAccess | |
dc.source | Clinical and Experimental Allergy | |
dc.subject | acetylation | en |
dc.subject | allergen-specific immunotherapy | en |
dc.subject | allergoid | en |
dc.subject | Art v 1 | en |
dc.subject | blocking antibodies | en |
dc.subject | mugwort pollen allergy | en |
dc.title | Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Перовиц, И.; ван Хаге, Марианне; Гафвелин, Гуро; Милчић-Матић, Наталија; Петровић, Д.; Буразер, Лидија М.; Станић, Драгана; Миловановиц, М.; Ћирковић-Величковић, Тања; Јанков, Ратко М.; | |
dc.citation.volume | 39 | |
dc.citation.issue | 3 | |
dc.citation.spage | 435 | |
dc.citation.epage | 446 | |
dc.citation.other | 39(3): 435-446 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 19178539 | |
dc.identifier.doi | 10.1111/j.1365-2222.2008.03158.x | |
dc.identifier.scopus | 2-s2.0-58849114494 | |
dc.identifier.wos | 000262879100018 | |
dc.type.version | publishedVersion | en |