Polyglycerol Ester-Based Low Energy Nanoemulsions with Red Raspberry Seed Oil and Fruit Extracts: Formulation Development toward Effective In Vitro/In Vivo Bioperformance
Аутори
Gledović, AnaJanošević Ležaić, Aleksandra
Nikolić, Ines
Tasić-Kostov, Marija Z.
Antić-Stanković, Jelena
Krstonošić, Veljko S.
Randjelović, Danijela
Božić, Dragana
Ilić, Dušan
Tamburić, Slobodanka D.
Savić, Snežana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions
based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO
and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions
were obtained via phase inversion composition (PIC) method at room temperature by dilution of
microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic,
rheological and differential scanning calorimetry (DSC) investigations. The results have shown that
the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from
red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet
size (Z-ave: 50 to 70 nm, PDI value _ 0.1). The highest antioxidant activity (~92% inhibition of the
DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic
antioxidants (FE), due to their syn...ergistic effect. The nanoemulsion carrier significantly increased
the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal
human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles
and significant improvement in skin hydration during 2 h after application for all nanoemulsions.
Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red
raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin
protection and hydration.
Кључне речи:
nanoemulsion / phase inversion composition (PIC) / biocompatible / low energy / polyglycerol ester / red raspberry seed oil / French oak fruit extract / antioxidant activity / MTT / skin hydrationИзвор:
Nanomaterials, 2021, 11, 1, 1-21Издавач:
- MDPI
Финансирање / пројекти:
- Развој микро- и наносистема као носача за лекове са антиинфламаторним деловањем и метода за њихову карактеризацију (RS-MESTD-Technological Development (TD or TR)-34031)
DOI: 10.3390/nano11010217
ISSN: 2079-4991
PubMed: 33467701
WoS: 000610664200001
Scopus: 2-s2.0-85100166444
Институција/група
IHTMTY - JOUR AU - Gledović, Ana AU - Janošević Ležaić, Aleksandra AU - Nikolić, Ines AU - Tasić-Kostov, Marija Z. AU - Antić-Stanković, Jelena AU - Krstonošić, Veljko S. AU - Randjelović, Danijela AU - Božić, Dragana AU - Ilić, Dušan AU - Tamburić, Slobodanka D. AU - Savić, Snežana PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4243 AB - This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and differential scanning calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value _ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration. PB - MDPI T2 - Nanomaterials T1 - Polyglycerol Ester-Based Low Energy Nanoemulsions with Red Raspberry Seed Oil and Fruit Extracts: Formulation Development toward Effective In Vitro/In Vivo Bioperformance VL - 11 IS - 1 SP - 1 EP - 21 DO - 10.3390/nano11010217 ER -
@article{ author = "Gledović, Ana and Janošević Ležaić, Aleksandra and Nikolić, Ines and Tasić-Kostov, Marija Z. and Antić-Stanković, Jelena and Krstonošić, Veljko S. and Randjelović, Danijela and Božić, Dragana and Ilić, Dušan and Tamburić, Slobodanka D. and Savić, Snežana", year = "2021", abstract = "This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and differential scanning calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value _ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration.", publisher = "MDPI", journal = "Nanomaterials", title = "Polyglycerol Ester-Based Low Energy Nanoemulsions with Red Raspberry Seed Oil and Fruit Extracts: Formulation Development toward Effective In Vitro/In Vivo Bioperformance", volume = "11", number = "1", pages = "1-21", doi = "10.3390/nano11010217" }
Gledović, A., Janošević Ležaić, A., Nikolić, I., Tasić-Kostov, M. Z., Antić-Stanković, J., Krstonošić, V. S., Randjelović, D., Božić, D., Ilić, D., Tamburić, S. D.,& Savić, S.. (2021). Polyglycerol Ester-Based Low Energy Nanoemulsions with Red Raspberry Seed Oil and Fruit Extracts: Formulation Development toward Effective In Vitro/In Vivo Bioperformance. in Nanomaterials MDPI., 11(1), 1-21. https://doi.org/10.3390/nano11010217
Gledović A, Janošević Ležaić A, Nikolić I, Tasić-Kostov MZ, Antić-Stanković J, Krstonošić VS, Randjelović D, Božić D, Ilić D, Tamburić SD, Savić S. Polyglycerol Ester-Based Low Energy Nanoemulsions with Red Raspberry Seed Oil and Fruit Extracts: Formulation Development toward Effective In Vitro/In Vivo Bioperformance. in Nanomaterials. 2021;11(1):1-21. doi:10.3390/nano11010217 .
Gledović, Ana, Janošević Ležaić, Aleksandra, Nikolić, Ines, Tasić-Kostov, Marija Z., Antić-Stanković, Jelena, Krstonošić, Veljko S., Randjelović, Danijela, Božić, Dragana, Ilić, Dušan, Tamburić, Slobodanka D., Savić, Snežana, "Polyglycerol Ester-Based Low Energy Nanoemulsions with Red Raspberry Seed Oil and Fruit Extracts: Formulation Development toward Effective In Vitro/In Vivo Bioperformance" in Nanomaterials, 11, no. 1 (2021):1-21, https://doi.org/10.3390/nano11010217 . .