Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?
Само за регистроване кориснике
2004
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). Study design: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. Results: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin- lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. Conclusion: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the forma...tion of Hb-Ch. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.
Кључне речи:
cholesterol / HDL cholesterol / hemoglobin / red blood cells / hemoglobin-lipid / seasonal variationИзвор:
Clinical Biochemistry, 2004, 37, 1, 22-26Издавач:
- Elsevier
DOI: 10.1016/j.clinbiochem.2003.10.002
ISSN: 0009-9120
PubMed: 14675558
WoS: 000187580700004
Scopus: 2-s2.0-0348111560
Институција/група
IHTMTY - JOUR AU - Nikolić, Milan AU - Stanić, Dragana AU - Antonijevic, Nadežda AU - Niketić, Vesna PY - 2004 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4167 AB - Objective: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). Study design: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. Results: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin- lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. Conclusion: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the formation of Hb-Ch. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved. PB - Elsevier T2 - Clinical Biochemistry T1 - Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation? VL - 37 IS - 1 SP - 22 EP - 26 DO - 10.1016/j.clinbiochem.2003.10.002 ER -
@article{ author = "Nikolić, Milan and Stanić, Dragana and Antonijevic, Nadežda and Niketić, Vesna", year = "2004", abstract = "Objective: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). Study design: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. Results: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin- lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. Conclusion: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the formation of Hb-Ch. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.", publisher = "Elsevier", journal = "Clinical Biochemistry", title = "Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?", volume = "37", number = "1", pages = "22-26", doi = "10.1016/j.clinbiochem.2003.10.002" }
Nikolić, M., Stanić, D., Antonijevic, N.,& Niketić, V.. (2004). Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?. in Clinical Biochemistry Elsevier., 37(1), 22-26. https://doi.org/10.1016/j.clinbiochem.2003.10.002
Nikolić M, Stanić D, Antonijevic N, Niketić V. Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?. in Clinical Biochemistry. 2004;37(1):22-26. doi:10.1016/j.clinbiochem.2003.10.002 .
Nikolić, Milan, Stanić, Dragana, Antonijevic, Nadežda, Niketić, Vesna, "Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?" in Clinical Biochemistry, 37, no. 1 (2004):22-26, https://doi.org/10.1016/j.clinbiochem.2003.10.002 . .