Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells
Samo za registrovane korisnike
2011
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In the present investigation the antiproliferative
activity of thirteen derivatives of betulinic acid and
betulin was tested against five different tumor cell lines.
The toxicity against normal human fibroblasts
(WWO70327) and the mode of cell death on HT-29
(colon cancer) as well as caspase activity induced by the
most active compounds, 9 (3-O-chloroacetylbetulinic
acid) and 15 (28-O-chloroacetylbetulin) were determined.
Investigated derivatives exerted a dose dependent antiproliferative
action at micromolar concentrations toward
target tumor cell lines. Treatment of HT-29 cells for 24 h
with 9 and 15 induced apoptosis, as observed by dye
exclusion test (trypan blue) and confirmed by the
appearance of a typical ladder pattern in the DNA
fragmentation assay.
Ključne reči:
Caspase activity / Cytotoxicity / Apoptosis / Natural products / Betulin / Betulinic acidIzvor:
Investigational New Drugs, 2011, 29, 2, 266-272Izdavač:
- Netherlands : Springer
Finansiranje / projekti:
- The authors are grateful to the Wirtschaftsministerium Sachsen-Anhalt for financial support (Antitumor drugs on the basis of natural compounds FuE 62/05).
DOI: 10.1007/s10637-009-9358-x
ISSN: 01676997; 1573-0646
PubMed: 19957199
WoS: 000287248100008
Scopus: 2-s2.0-79955594006
Institucija/grupa
IHTMTY - JOUR AU - Kommera, Harish AU - Kaluđerović, Goran N. AU - Kalbitz, Jutta AU - Paschke, Reinhard PY - 2011 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3411 AB - In the present investigation the antiproliferative activity of thirteen derivatives of betulinic acid and betulin was tested against five different tumor cell lines. The toxicity against normal human fibroblasts (WWO70327) and the mode of cell death on HT-29 (colon cancer) as well as caspase activity induced by the most active compounds, 9 (3-O-chloroacetylbetulinic acid) and 15 (28-O-chloroacetylbetulin) were determined. Investigated derivatives exerted a dose dependent antiproliferative action at micromolar concentrations toward target tumor cell lines. Treatment of HT-29 cells for 24 h with 9 and 15 induced apoptosis, as observed by dye exclusion test (trypan blue) and confirmed by the appearance of a typical ladder pattern in the DNA fragmentation assay. PB - Netherlands : Springer T2 - Investigational New Drugs T1 - Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells VL - 29 IS - 2 SP - 266 EP - 272 DO - 10.1007/s10637-009-9358-x ER -
@article{ author = "Kommera, Harish and Kaluđerović, Goran N. and Kalbitz, Jutta and Paschke, Reinhard", year = "2011", abstract = "In the present investigation the antiproliferative activity of thirteen derivatives of betulinic acid and betulin was tested against five different tumor cell lines. The toxicity against normal human fibroblasts (WWO70327) and the mode of cell death on HT-29 (colon cancer) as well as caspase activity induced by the most active compounds, 9 (3-O-chloroacetylbetulinic acid) and 15 (28-O-chloroacetylbetulin) were determined. Investigated derivatives exerted a dose dependent antiproliferative action at micromolar concentrations toward target tumor cell lines. Treatment of HT-29 cells for 24 h with 9 and 15 induced apoptosis, as observed by dye exclusion test (trypan blue) and confirmed by the appearance of a typical ladder pattern in the DNA fragmentation assay.", publisher = "Netherlands : Springer", journal = "Investigational New Drugs", title = "Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells", volume = "29", number = "2", pages = "266-272", doi = "10.1007/s10637-009-9358-x" }
Kommera, H., Kaluđerović, G. N., Kalbitz, J.,& Paschke, R.. (2011). Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells. in Investigational New Drugs Netherlands : Springer., 29(2), 266-272. https://doi.org/10.1007/s10637-009-9358-x
Kommera H, Kaluđerović GN, Kalbitz J, Paschke R. Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells. in Investigational New Drugs. 2011;29(2):266-272. doi:10.1007/s10637-009-9358-x .
Kommera, Harish, Kaluđerović, Goran N., Kalbitz, Jutta, Paschke, Reinhard, "Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells" in Investigational New Drugs, 29, no. 2 (2011):266-272, https://doi.org/10.1007/s10637-009-9358-x . .