pH-Dependent solubility profile of desipramine hydrochloride
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pH-dependent solubility profile of Desipramine hydrochloride (Ds.HCl) was studied using pH-ramp shake flask method. First, the pH value of DsHCl stock solution in 0.15 M phosphate buffer was adjusted to 11.7 in order to minimize supersaturation effect. Then, the pH value in separate samples was adjusted downwards with HCl, to prepare solutions in the pH 1.7-11.7 region. After stirring (6 h) and sedimentation (18 h), PTFE (hydrophobic, pore size 0.22 µm) filters or centrifugation were used for phase separation. Concentration was measured using HPLC with UV/Vis detection. The computer program pDISOL-X was used for data processing and refinement of equilibrium constants. Different techniques were used for solid phase characterization.
Keywords:
pH-dependent solubility / Desipramine hydrochloride / pH-ramp shake flask methodSource:
6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts, 2017, 42-42Publisher:
- International Association of Physical Chemists
Funding / projects:
- Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology (RS-172035)
- The synthesis of aminoquinoline-based antimalarials and botulinum neurotoxin A inhibitors (RS-172008)
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IHTMTY - CONF AU - Marković, Olivera AU - Pešić, Miloš P. AU - Verbić, Tatjana AU - Avdeef, Alex PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3402 AB - pH-dependent solubility profile of Desipramine hydrochloride (Ds.HCl) was studied using pH-ramp shake flask method. First, the pH value of DsHCl stock solution in 0.15 M phosphate buffer was adjusted to 11.7 in order to minimize supersaturation effect. Then, the pH value in separate samples was adjusted downwards with HCl, to prepare solutions in the pH 1.7-11.7 region. After stirring (6 h) and sedimentation (18 h), PTFE (hydrophobic, pore size 0.22 µm) filters or centrifugation were used for phase separation. Concentration was measured using HPLC with UV/Vis detection. The computer program pDISOL-X was used for data processing and refinement of equilibrium constants. Different techniques were used for solid phase characterization. PB - International Association of Physical Chemists C3 - 6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts T1 - pH-Dependent solubility profile of desipramine hydrochloride SP - 42 EP - 42 UR - https://hdl.handle.net/21.15107/rcub_cer_3402 ER -
@conference{ author = "Marković, Olivera and Pešić, Miloš P. and Verbić, Tatjana and Avdeef, Alex", year = "2017", abstract = "pH-dependent solubility profile of Desipramine hydrochloride (Ds.HCl) was studied using pH-ramp shake flask method. First, the pH value of DsHCl stock solution in 0.15 M phosphate buffer was adjusted to 11.7 in order to minimize supersaturation effect. Then, the pH value in separate samples was adjusted downwards with HCl, to prepare solutions in the pH 1.7-11.7 region. After stirring (6 h) and sedimentation (18 h), PTFE (hydrophobic, pore size 0.22 µm) filters or centrifugation were used for phase separation. Concentration was measured using HPLC with UV/Vis detection. The computer program pDISOL-X was used for data processing and refinement of equilibrium constants. Different techniques were used for solid phase characterization.", publisher = "International Association of Physical Chemists", journal = "6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts", title = "pH-Dependent solubility profile of desipramine hydrochloride", pages = "42-42", url = "https://hdl.handle.net/21.15107/rcub_cer_3402" }
Marković, O., Pešić, M. P., Verbić, T.,& Avdeef, A.. (2017). pH-Dependent solubility profile of desipramine hydrochloride. in 6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts International Association of Physical Chemists., 42-42. https://hdl.handle.net/21.15107/rcub_cer_3402
Marković O, Pešić MP, Verbić T, Avdeef A. pH-Dependent solubility profile of desipramine hydrochloride. in 6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts. 2017;:42-42. https://hdl.handle.net/21.15107/rcub_cer_3402 .
Marković, Olivera, Pešić, Miloš P., Verbić, Tatjana, Avdeef, Alex, "pH-Dependent solubility profile of desipramine hydrochloride" in 6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts (2017):42-42, https://hdl.handle.net/21.15107/rcub_cer_3402 .