dc.creator | Dimitrijević, Milena S. | |
dc.creator | Bogdanović Pristov, Jelena | |
dc.creator | Žižić, Milan | |
dc.creator | Stanković, Dalibor | |
dc.creator | Bajuk-Bogdanović, Danica | |
dc.creator | Stanić, Marina | |
dc.creator | Spasić, Snežana | |
dc.creator | Hagen, Wilfred | |
dc.creator | Spasojević, Ivan | |
dc.date.accessioned | 2019-06-03T08:18:31Z | |
dc.date.available | 2020-02-04 | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1477-9226 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/2933 | |
dc.description.abstract | Biliverdin (BV), a product of heme catabolism, is known to interact with transition metals, but the details of such interactions under physiological conditions are scarce. Herein, we examined coordinate/redox interactions of BV with Cu2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman spectroscopy, 1 H NMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable coordination complex with copper in 1 : 1 stoichiometry. The structure of BV was more planar and energetically stable in the complex. The complex showed strong paramagnetic effects that were attributed to an unpaired delocalized e−. The delocalized electron may come from BV or Cu2+, so the complex is formally composed either of BV radical cation and Cu1+ or of BV radical anion and Cu3+. The complex underwent oxidation only in the presence of both O2 and an excess of Cu2+, or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43010/RS// | |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/621375/EU// | |
dc.rights | embargoedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Dalton Transactions | |
dc.title | Biliverdin-copper complex at physiological pH | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Бајук-Богдановић, Даница; Димитријевић, Милена С.; Богдановић Пристов, Јелена; Жижић, Милан; Станковић, Далибор; Станић, Марина; Спасић, Снежана; Хаген, Wилфред; Спасојевић, Иван; | |
dc.citation.volume | 48 | |
dc.citation.issue | 18 | |
dc.citation.spage | 6061 | |
dc.citation.epage | 6070 | |
dc.citation.rank | M21~ | |
dc.description.other | This is the peer-reviewed version of the following article: Dimitrijević, M. S.; Bogdanović Pristov, J.; Žižić, M.; Stanković, D. M.; Bajuk-Bogdanović, D.; Stanić, M.; Spasić, S.; Hagen, W.; Spasojević, I. Biliverdin-Copper Complex at Physiological PH. Dalton Transactions 2019, 48 (18), 6061–6070. [https://doi.org/10.1039/c8dt04724c] | |
dc.description.other | [http://cer.ihtm.bg.ac.rs/handle/123456789/2932] | |
dc.identifier.doi | 10.1039/c8dt04724c | |
dc.identifier.fulltext | https://cer.ihtm.bg.ac.rs/bitstream/id/7135/bitstream_7135.pdf | |
dc.identifier.scopus | 2-s2.0-85065545631 | |
dc.identifier.wos | 000472449300020 | |
dc.type.version | acceptedVersion | |