Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach
Abstract
Herein, we propose a H-1 NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H-1 and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberine and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity.
Keywords:
Cytotoxic activity / NMR / Metabolomics / Mahonia aquifoliumSource:
Journal of Pharmaceutical and Biomedical Analysis, 2018, 150, 9-14Publisher:
- Elsevier
Funding / projects:
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)
- Biological response modifiers in physiological and pathological conditions (RS-175011)
DOI: 10.1016/j.jpba.2017.11.075
ISSN: 0731-7085
PubMed: 29202306
WoS: 000423497000002
Scopus: 2-s2.0-85036522547
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Institution/Community
IHTMTY - JOUR AU - Gođevac, Dejan AU - Damjanovic, Ana AU - Stanojković, Tatjana AU - Anđelković, Boban D. AU - Zdunić, Gordana PY - 2018 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2464 AB - Herein, we propose a H-1 NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H-1 and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberine and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity. PB - Elsevier T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach VL - 150 SP - 9 EP - 14 DO - 10.1016/j.jpba.2017.11.075 ER -
@article{ author = "Gođevac, Dejan and Damjanovic, Ana and Stanojković, Tatjana and Anđelković, Boban D. and Zdunić, Gordana", year = "2018", abstract = "Herein, we propose a H-1 NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H-1 and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberine and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity.", publisher = "Elsevier", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach", volume = "150", pages = "9-14", doi = "10.1016/j.jpba.2017.11.075" }
Gođevac, D., Damjanovic, A., Stanojković, T., Anđelković, B. D.,& Zdunić, G.. (2018). Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach. in Journal of Pharmaceutical and Biomedical Analysis Elsevier., 150, 9-14. https://doi.org/10.1016/j.jpba.2017.11.075
Gođevac D, Damjanovic A, Stanojković T, Anđelković BD, Zdunić G. Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach. in Journal of Pharmaceutical and Biomedical Analysis. 2018;150:9-14. doi:10.1016/j.jpba.2017.11.075 .
Gođevac, Dejan, Damjanovic, Ana, Stanojković, Tatjana, Anđelković, Boban D., Zdunić, Gordana, "Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach" in Journal of Pharmaceutical and Biomedical Analysis, 150 (2018):9-14, https://doi.org/10.1016/j.jpba.2017.11.075 . .