Use of monolithic supports for high-throughput protein and peptide separation in proteomics
Samo za registrovane korisnike
2017
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biologica...l samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.
Ključne reči:
High-throughput / Liquid chromatography / Mass spectrometry / Monoliths / ProteomicsIzvor:
Electrophoresis, 2017, 38, 22-23, 2851-2869Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-172048)
- Interakcije prirodnih proizvoda, njihovih derivata i kompleksnih jedinjenja sa proteinima i nukleinskim kiselinama (RS-172055)
- Alergeni, antitela, enzimi i mali fiziološki značajni molekuli: dizajn, struktura, funkcija i značaj (RS-172049)
- HTP-GLYCOMET - Methods for high-throughput glycoproteomic analysis (EU-324400)
Napomena:
- Peer-reviewed version: http://cer.ihtm.bg.ac.rs/handle/123456789/2985
DOI: 10.1002/elps.201700260
ISSN: 0173-0835
PubMed: 28906564
WoS: 000416867300006
Scopus: 2-s2.0-85036597546
Institucija/grupa
IHTMTY - JOUR AU - Anđelković, Uroš AU - Tufegdžić, Srđan AU - Popović, Milica PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2122 AB - The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed. PB - Wiley, Hoboken T2 - Electrophoresis T1 - Use of monolithic supports for high-throughput protein and peptide separation in proteomics VL - 38 IS - 22-23 SP - 2851 EP - 2869 DO - 10.1002/elps.201700260 ER -
@article{ author = "Anđelković, Uroš and Tufegdžić, Srđan and Popović, Milica", year = "2017", abstract = "The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.", publisher = "Wiley, Hoboken", journal = "Electrophoresis", title = "Use of monolithic supports for high-throughput protein and peptide separation in proteomics", volume = "38", number = "22-23", pages = "2851-2869", doi = "10.1002/elps.201700260" }
Anđelković, U., Tufegdžić, S.,& Popović, M.. (2017). Use of monolithic supports for high-throughput protein and peptide separation in proteomics. in Electrophoresis Wiley, Hoboken., 38(22-23), 2851-2869. https://doi.org/10.1002/elps.201700260
Anđelković U, Tufegdžić S, Popović M. Use of monolithic supports for high-throughput protein and peptide separation in proteomics. in Electrophoresis. 2017;38(22-23):2851-2869. doi:10.1002/elps.201700260 .
Anđelković, Uroš, Tufegdžić, Srđan, Popović, Milica, "Use of monolithic supports for high-throughput protein and peptide separation in proteomics" in Electrophoresis, 38, no. 22-23 (2017):2851-2869, https://doi.org/10.1002/elps.201700260 . .