Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita
Апстракт
This work presents the electrochemical behavior of levodopa (L-DOPA), at boron-doped diamond (BDD) electrodes, using cycling voltammetry (CV), in Britton-Robinson (BR) buffer solution, and application of the proposed electrode for the determination of L-DOPA in extracts from the seeds of velvet bean (Mucuna prurita Hook or Mucuna pruriens (L.) DC.). L-DOPA provides a well-defined and single oval-shape oxidation peak at +0.8 V vs. Ag/AgCl (3 M KCl) reference electrode in BR buffer solution at pH 3.0. Experimental parameters, such as pH of supporting electrolyte and square wave voltammetry (SWV) operating parameters (frequency and modulation amplitude) were optimized. The effect of possible interferences was evaluated. Under optimal conditions the detection limit of the developed method was 0.8 mu M and the calibration curve for L-DOPA was linear in the range from 2 to 100 mu M. The proposed method was successfully applied to the determination of L-DOPA in an extract from the seeds of Mu...cuna prurita. The obtained result was in good agreement with obtained by photometry with 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). The developed approach can be beneficial for the quantification of L-DOPA using a BDD electrode as up-to-date potential alternative sensor for electroanalytical applications.
Кључне речи:
L-DOPA / boron-doped diamond electrode / square wave voltammetryИзвор:
Acta Chimica Slovenica, 2016, 63, 2, 220-226Издавач:
- Slovensko Kemijsko Drustvo, Ljubljana
Финансирање / пројекти:
- Примена унапређених оксидационих процеса и наноструктурисаних оксидних материјала за уклањање загађивача из животне средине, развој и оптимизација инструменталних техника за праћење ефикасности (RS-172030)
- Asea UniNet
- JoinEU-SEE-Penta Erasmus Mundus scholarship
DOI: 10.17344/acsi.2015.1541
ISSN: 1318-0207
PubMed: 27333543
WoS: 000388252900003
Scopus: 2-s2.0-84975259896
Институција/група
IHTMTY - JOUR AU - Stanković, Dalibor AU - Samphao, Anchalee AU - Dojčinović, Biljana AU - Kalcher, Kurt PY - 2016 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1997 AB - This work presents the electrochemical behavior of levodopa (L-DOPA), at boron-doped diamond (BDD) electrodes, using cycling voltammetry (CV), in Britton-Robinson (BR) buffer solution, and application of the proposed electrode for the determination of L-DOPA in extracts from the seeds of velvet bean (Mucuna prurita Hook or Mucuna pruriens (L.) DC.). L-DOPA provides a well-defined and single oval-shape oxidation peak at +0.8 V vs. Ag/AgCl (3 M KCl) reference electrode in BR buffer solution at pH 3.0. Experimental parameters, such as pH of supporting electrolyte and square wave voltammetry (SWV) operating parameters (frequency and modulation amplitude) were optimized. The effect of possible interferences was evaluated. Under optimal conditions the detection limit of the developed method was 0.8 mu M and the calibration curve for L-DOPA was linear in the range from 2 to 100 mu M. The proposed method was successfully applied to the determination of L-DOPA in an extract from the seeds of Mucuna prurita. The obtained result was in good agreement with obtained by photometry with 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). The developed approach can be beneficial for the quantification of L-DOPA using a BDD electrode as up-to-date potential alternative sensor for electroanalytical applications. PB - Slovensko Kemijsko Drustvo, Ljubljana T2 - Acta Chimica Slovenica T1 - Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita VL - 63 IS - 2 SP - 220 EP - 226 DO - 10.17344/acsi.2015.1541 ER -
@article{ author = "Stanković, Dalibor and Samphao, Anchalee and Dojčinović, Biljana and Kalcher, Kurt", year = "2016", abstract = "This work presents the electrochemical behavior of levodopa (L-DOPA), at boron-doped diamond (BDD) electrodes, using cycling voltammetry (CV), in Britton-Robinson (BR) buffer solution, and application of the proposed electrode for the determination of L-DOPA in extracts from the seeds of velvet bean (Mucuna prurita Hook or Mucuna pruriens (L.) DC.). L-DOPA provides a well-defined and single oval-shape oxidation peak at +0.8 V vs. Ag/AgCl (3 M KCl) reference electrode in BR buffer solution at pH 3.0. Experimental parameters, such as pH of supporting electrolyte and square wave voltammetry (SWV) operating parameters (frequency and modulation amplitude) were optimized. The effect of possible interferences was evaluated. Under optimal conditions the detection limit of the developed method was 0.8 mu M and the calibration curve for L-DOPA was linear in the range from 2 to 100 mu M. The proposed method was successfully applied to the determination of L-DOPA in an extract from the seeds of Mucuna prurita. The obtained result was in good agreement with obtained by photometry with 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). The developed approach can be beneficial for the quantification of L-DOPA using a BDD electrode as up-to-date potential alternative sensor for electroanalytical applications.", publisher = "Slovensko Kemijsko Drustvo, Ljubljana", journal = "Acta Chimica Slovenica", title = "Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita", volume = "63", number = "2", pages = "220-226", doi = "10.17344/acsi.2015.1541" }
Stanković, D., Samphao, A., Dojčinović, B.,& Kalcher, K.. (2016). Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita. in Acta Chimica Slovenica Slovensko Kemijsko Drustvo, Ljubljana., 63(2), 220-226. https://doi.org/10.17344/acsi.2015.1541
Stanković D, Samphao A, Dojčinović B, Kalcher K. Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita. in Acta Chimica Slovenica. 2016;63(2):220-226. doi:10.17344/acsi.2015.1541 .
Stanković, Dalibor, Samphao, Anchalee, Dojčinović, Biljana, Kalcher, Kurt, "Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita" in Acta Chimica Slovenica, 63, no. 2 (2016):220-226, https://doi.org/10.17344/acsi.2015.1541 . .