Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization
Само за регистроване кориснике
2015
Аутори
Prodanović, OliveraSpasojevic, Dragica
Prokopijevic, Milos
Radotić, Ksenija
Marković, Nevena
Blažić, Marija
Prodanović, Radivoje
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Phenol and amino groups were introduced into alginate to different degrees via oxidation with 2.5, 5, 10, 15 and 20 mol% of periodate and reductive amination by tyramine. Modification of alginate with tyramine was confirmed by FTIR spectroscopy and UV-VIS spectroscopy, while concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid-base titration. All tyramine-alginates were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. Tyramine-alginates oxidized with up to 10 mol% of periodate were also capable of forming hydrogels with calcium ions. Tyramine-alginates were tested for HRP immobilization within micro-beads obtained by peroxidase catalyzed droplet polymerization using internal delivery of hydrogen peroxide via glucose oxidase and glucose. Highest activity of immobilized peroxidase was obtained with 20% (w/v) tyramine-alginate obtained via 20 mol% periodate oxidation. Immobilized enzyme was not ...leaking from the micro-beads and was further kinetically characterized for pyrogallol oxidation. Km for pyrogallol was increased after immobilization from 1.93 mM for soluble HRP to 734 mM for immobilized HRP. The optimum pH was also increased from pH 7.0 to 8.0. Temperature and organic solvent stability improved significantly after immobilization, so that half-life at 70 degrees C increased around four times, while half-life in 80% (v/v) dioxane increased 22 times. After repeated use of 6 times in batch reactor for pyrogallol oxidation immobilized HRP retained 45% of original activity.
Кључне речи:
Hydrogel / Tyramine / Polymerization / Emulsion / PeroxideИзвор:
Reactive and Functional Polymers, 2015, 93, 77-83Издавач:
- Elsevier
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-173017)
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-172049)
DOI: 10.1016/j.reactfunctpolym.2015.06.004
ISSN: 1381-5148
WoS: 000360869200009
Scopus: 2-s2.0-84935909709
Институција/група
IHTMTY - JOUR AU - Prodanović, Olivera AU - Spasojevic, Dragica AU - Prokopijevic, Milos AU - Radotić, Ksenija AU - Marković, Nevena AU - Blažić, Marija AU - Prodanović, Radivoje PY - 2015 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1727 AB - Phenol and amino groups were introduced into alginate to different degrees via oxidation with 2.5, 5, 10, 15 and 20 mol% of periodate and reductive amination by tyramine. Modification of alginate with tyramine was confirmed by FTIR spectroscopy and UV-VIS spectroscopy, while concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid-base titration. All tyramine-alginates were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. Tyramine-alginates oxidized with up to 10 mol% of periodate were also capable of forming hydrogels with calcium ions. Tyramine-alginates were tested for HRP immobilization within micro-beads obtained by peroxidase catalyzed droplet polymerization using internal delivery of hydrogen peroxide via glucose oxidase and glucose. Highest activity of immobilized peroxidase was obtained with 20% (w/v) tyramine-alginate obtained via 20 mol% periodate oxidation. Immobilized enzyme was not leaking from the micro-beads and was further kinetically characterized for pyrogallol oxidation. Km for pyrogallol was increased after immobilization from 1.93 mM for soluble HRP to 734 mM for immobilized HRP. The optimum pH was also increased from pH 7.0 to 8.0. Temperature and organic solvent stability improved significantly after immobilization, so that half-life at 70 degrees C increased around four times, while half-life in 80% (v/v) dioxane increased 22 times. After repeated use of 6 times in batch reactor for pyrogallol oxidation immobilized HRP retained 45% of original activity. PB - Elsevier T2 - Reactive and Functional Polymers T1 - Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization VL - 93 SP - 77 EP - 83 DO - 10.1016/j.reactfunctpolym.2015.06.004 ER -
@article{ author = "Prodanović, Olivera and Spasojevic, Dragica and Prokopijevic, Milos and Radotić, Ksenija and Marković, Nevena and Blažić, Marija and Prodanović, Radivoje", year = "2015", abstract = "Phenol and amino groups were introduced into alginate to different degrees via oxidation with 2.5, 5, 10, 15 and 20 mol% of periodate and reductive amination by tyramine. Modification of alginate with tyramine was confirmed by FTIR spectroscopy and UV-VIS spectroscopy, while concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid-base titration. All tyramine-alginates were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. Tyramine-alginates oxidized with up to 10 mol% of periodate were also capable of forming hydrogels with calcium ions. Tyramine-alginates were tested for HRP immobilization within micro-beads obtained by peroxidase catalyzed droplet polymerization using internal delivery of hydrogen peroxide via glucose oxidase and glucose. Highest activity of immobilized peroxidase was obtained with 20% (w/v) tyramine-alginate obtained via 20 mol% periodate oxidation. Immobilized enzyme was not leaking from the micro-beads and was further kinetically characterized for pyrogallol oxidation. Km for pyrogallol was increased after immobilization from 1.93 mM for soluble HRP to 734 mM for immobilized HRP. The optimum pH was also increased from pH 7.0 to 8.0. Temperature and organic solvent stability improved significantly after immobilization, so that half-life at 70 degrees C increased around four times, while half-life in 80% (v/v) dioxane increased 22 times. After repeated use of 6 times in batch reactor for pyrogallol oxidation immobilized HRP retained 45% of original activity.", publisher = "Elsevier", journal = "Reactive and Functional Polymers", title = "Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization", volume = "93", pages = "77-83", doi = "10.1016/j.reactfunctpolym.2015.06.004" }
Prodanović, O., Spasojevic, D., Prokopijevic, M., Radotić, K., Marković, N., Blažić, M.,& Prodanović, R.. (2015). Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization. in Reactive and Functional Polymers Elsevier., 93, 77-83. https://doi.org/10.1016/j.reactfunctpolym.2015.06.004
Prodanović O, Spasojevic D, Prokopijevic M, Radotić K, Marković N, Blažić M, Prodanović R. Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization. in Reactive and Functional Polymers. 2015;93:77-83. doi:10.1016/j.reactfunctpolym.2015.06.004 .
Prodanović, Olivera, Spasojevic, Dragica, Prokopijevic, Milos, Radotić, Ksenija, Marković, Nevena, Blažić, Marija, Prodanović, Radivoje, "Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization" in Reactive and Functional Polymers, 93 (2015):77-83, https://doi.org/10.1016/j.reactfunctpolym.2015.06.004 . .