Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization
Само за регистроване кориснике
2013
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
One hundred wild type strains of Bacillus sp. were isolated from industrial and agricultural soil across Serbia and screened for laccase activity. Three strains showed high laccase activity temperature optimum of 65 and 80 degrees C towards ABTS. A new laccase gene from the strain with highest temperature optimum, namely Bacillus amyloliquefaciens 12B was cloned and expressed in Escherichia coli. Recombinant laccase degraded dye Reactive blue 52 at pH 7.0 and pH 4.0 and at elevated temperature, while fungal laccases was unable to act on this substrate at pH higher than 4.0 and was quickly inactivated at temperatures higher than 45 degrees C. Degradation of dye was monitored by HPLC-DAD and resulting precipitate was analyzed by FTIR spectroscopy. Single product peak without chromophore was detected in solution, while water insoluble aggregate, presumably dye polymer is formed retaining blue color.
Кључне речи:
Laccase / Bacillus amyloliquefaciens / Decolorization / BiodegradationИзвор:
Bioresource Technology, 2013, 147, 177-183Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Производња, изоловање и карактеризација ензима и малих молекула и њихова примена у растворном и имобилизованом облику у биотехнологији хране, биогоривима и заштитити животне средине (RS-MESTD-Basic Research (BR or ON)-172048)
- Joint Serbian-Spanish Action
- Spanish MICINN [CTQ2011-28398-CO2-01]
DOI: 10.1016/j.biortech.2013.08.056
ISSN: 0960-8524
PubMed: 23994699
WoS: 000326660900026
Scopus: 2-s2.0-84883380664
Институција/група
IHTMTY - JOUR AU - Lončar, Nikola AU - Božić, Nataša AU - Lopez-Santin, Josep AU - Vujčić, Zoran PY - 2013 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1256 AB - One hundred wild type strains of Bacillus sp. were isolated from industrial and agricultural soil across Serbia and screened for laccase activity. Three strains showed high laccase activity temperature optimum of 65 and 80 degrees C towards ABTS. A new laccase gene from the strain with highest temperature optimum, namely Bacillus amyloliquefaciens 12B was cloned and expressed in Escherichia coli. Recombinant laccase degraded dye Reactive blue 52 at pH 7.0 and pH 4.0 and at elevated temperature, while fungal laccases was unable to act on this substrate at pH higher than 4.0 and was quickly inactivated at temperatures higher than 45 degrees C. Degradation of dye was monitored by HPLC-DAD and resulting precipitate was analyzed by FTIR spectroscopy. Single product peak without chromophore was detected in solution, while water insoluble aggregate, presumably dye polymer is formed retaining blue color. PB - Elsevier Sci Ltd, Oxford T2 - Bioresource Technology T1 - Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization VL - 147 SP - 177 EP - 183 DO - 10.1016/j.biortech.2013.08.056 ER -
@article{ author = "Lončar, Nikola and Božić, Nataša and Lopez-Santin, Josep and Vujčić, Zoran", year = "2013", abstract = "One hundred wild type strains of Bacillus sp. were isolated from industrial and agricultural soil across Serbia and screened for laccase activity. Three strains showed high laccase activity temperature optimum of 65 and 80 degrees C towards ABTS. A new laccase gene from the strain with highest temperature optimum, namely Bacillus amyloliquefaciens 12B was cloned and expressed in Escherichia coli. Recombinant laccase degraded dye Reactive blue 52 at pH 7.0 and pH 4.0 and at elevated temperature, while fungal laccases was unable to act on this substrate at pH higher than 4.0 and was quickly inactivated at temperatures higher than 45 degrees C. Degradation of dye was monitored by HPLC-DAD and resulting precipitate was analyzed by FTIR spectroscopy. Single product peak without chromophore was detected in solution, while water insoluble aggregate, presumably dye polymer is formed retaining blue color.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Bioresource Technology", title = "Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization", volume = "147", pages = "177-183", doi = "10.1016/j.biortech.2013.08.056" }
Lončar, N., Božić, N., Lopez-Santin, J.,& Vujčić, Z.. (2013). Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization. in Bioresource Technology Elsevier Sci Ltd, Oxford., 147, 177-183. https://doi.org/10.1016/j.biortech.2013.08.056
Lončar N, Božić N, Lopez-Santin J, Vujčić Z. Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization. in Bioresource Technology. 2013;147:177-183. doi:10.1016/j.biortech.2013.08.056 .
Lončar, Nikola, Božić, Nataša, Lopez-Santin, Josep, Vujčić, Zoran, "Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization" in Bioresource Technology, 147 (2013):177-183, https://doi.org/10.1016/j.biortech.2013.08.056 . .