Composition, antifungal and antioxidant properties of Hyssopus officinalis L. subsp pilifer (Pant.) Murb. essential oil and deodorized extracts
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2013
Authors
Dzamic, Ana M.Soković, Marina
Novaković, Miroslav
Jadranin, Milka
Ristic, Mihailo S.
Tešević, Vele
Marin, Petar D.
Article (Published version)
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The aim of the study was to investigate the chemical composition of Hyssopus officinalis L subsp. pilifer (Pant.) Murb. essential oil (EO) and deodorized extracts (DE) and to evaluate their potential antifungal and antioxidant activities. EO was analyzed by GC-FID and GC-MS. Among the 30 compounds identified in the oil, the main were 1.8-cineole (36.43%), beta-pinene (19.55%), isopinocamphone (15.32%) and trans-pinocamphone (6.39%). Deodorized aqueous extract (DAE) was analyzed by LC-DAD/ESI-TOF MS. The most significant phenolic acids detected in DAE were syringic, caffeoylqunic acids, feruloylquinic and rosmarinic acid. Aspergillus niger, A. ochraceus, A. versicolor, A. fumigatus, Cladosporium cladosporioides, C. fulvum, Penicillium funiculosum, P. ochrochloron, Trichoderma viride and Candida albicans yeast were used for antifungal assay. Minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of the tested EO and DEs were recorded by the microdilution me...thod. The antioxidant potential of the extracts was evaluated by means of the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging method. The deodorized aqueous extract (DAE) possessed the highest scavenging activity (EC50=0.54 mg/ml) followed by the deodorized methanol extract (DME) (EC50=0.82 mg/ml) and deodorized ethyl acetate extract (DEE) (EC50 = 2.97 mg/ml). EC/possessed the lowest activities compared to other extracts and control substances. Total phenolic content of DEs was determined by Folin-Ciocalteau (FC) assay and gallic acid was used as the standard. DAE of H. officinalis subsp. pilifer exhibited the highest phenolic content 96.47 GAE.
Keywords:
Hyssopus officinalis subsp pilifer / Essential oil / Deodorized extracts / Antifungal activity / Antioxidant activitySource:
Industrial Crops and Products, 2013, 51, 401-407Publisher:
- Elsevier
Funding / projects:
- Micromorphological, phytochemical and molecular investigations of plants - systematic, ecological and applicative aspects (RS-MESTD-Basic Research (BR or ON)-173029)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.1016/j.indcrop.2013.09.038
ISSN: 0926-6690
WoS: 000330820800056
Scopus: 2-s2.0-84886837939
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IHTMTY - JOUR AU - Dzamic, Ana M. AU - Soković, Marina AU - Novaković, Miroslav AU - Jadranin, Milka AU - Ristic, Mihailo S. AU - Tešević, Vele AU - Marin, Petar D. PY - 2013 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1242 AB - The aim of the study was to investigate the chemical composition of Hyssopus officinalis L subsp. pilifer (Pant.) Murb. essential oil (EO) and deodorized extracts (DE) and to evaluate their potential antifungal and antioxidant activities. EO was analyzed by GC-FID and GC-MS. Among the 30 compounds identified in the oil, the main were 1.8-cineole (36.43%), beta-pinene (19.55%), isopinocamphone (15.32%) and trans-pinocamphone (6.39%). Deodorized aqueous extract (DAE) was analyzed by LC-DAD/ESI-TOF MS. The most significant phenolic acids detected in DAE were syringic, caffeoylqunic acids, feruloylquinic and rosmarinic acid. Aspergillus niger, A. ochraceus, A. versicolor, A. fumigatus, Cladosporium cladosporioides, C. fulvum, Penicillium funiculosum, P. ochrochloron, Trichoderma viride and Candida albicans yeast were used for antifungal assay. Minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of the tested EO and DEs were recorded by the microdilution method. The antioxidant potential of the extracts was evaluated by means of the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging method. The deodorized aqueous extract (DAE) possessed the highest scavenging activity (EC50=0.54 mg/ml) followed by the deodorized methanol extract (DME) (EC50=0.82 mg/ml) and deodorized ethyl acetate extract (DEE) (EC50 = 2.97 mg/ml). EC/possessed the lowest activities compared to other extracts and control substances. Total phenolic content of DEs was determined by Folin-Ciocalteau (FC) assay and gallic acid was used as the standard. DAE of H. officinalis subsp. pilifer exhibited the highest phenolic content 96.47 GAE. PB - Elsevier T2 - Industrial Crops and Products T1 - Composition, antifungal and antioxidant properties of Hyssopus officinalis L. subsp pilifer (Pant.) Murb. essential oil and deodorized extracts VL - 51 SP - 401 EP - 407 DO - 10.1016/j.indcrop.2013.09.038 ER -
@article{ author = "Dzamic, Ana M. and Soković, Marina and Novaković, Miroslav and Jadranin, Milka and Ristic, Mihailo S. and Tešević, Vele and Marin, Petar D.", year = "2013", abstract = "The aim of the study was to investigate the chemical composition of Hyssopus officinalis L subsp. pilifer (Pant.) Murb. essential oil (EO) and deodorized extracts (DE) and to evaluate their potential antifungal and antioxidant activities. EO was analyzed by GC-FID and GC-MS. Among the 30 compounds identified in the oil, the main were 1.8-cineole (36.43%), beta-pinene (19.55%), isopinocamphone (15.32%) and trans-pinocamphone (6.39%). Deodorized aqueous extract (DAE) was analyzed by LC-DAD/ESI-TOF MS. The most significant phenolic acids detected in DAE were syringic, caffeoylqunic acids, feruloylquinic and rosmarinic acid. Aspergillus niger, A. ochraceus, A. versicolor, A. fumigatus, Cladosporium cladosporioides, C. fulvum, Penicillium funiculosum, P. ochrochloron, Trichoderma viride and Candida albicans yeast were used for antifungal assay. Minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of the tested EO and DEs were recorded by the microdilution method. The antioxidant potential of the extracts was evaluated by means of the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging method. The deodorized aqueous extract (DAE) possessed the highest scavenging activity (EC50=0.54 mg/ml) followed by the deodorized methanol extract (DME) (EC50=0.82 mg/ml) and deodorized ethyl acetate extract (DEE) (EC50 = 2.97 mg/ml). EC/possessed the lowest activities compared to other extracts and control substances. Total phenolic content of DEs was determined by Folin-Ciocalteau (FC) assay and gallic acid was used as the standard. DAE of H. officinalis subsp. pilifer exhibited the highest phenolic content 96.47 GAE.", publisher = "Elsevier", journal = "Industrial Crops and Products", title = "Composition, antifungal and antioxidant properties of Hyssopus officinalis L. subsp pilifer (Pant.) Murb. essential oil and deodorized extracts", volume = "51", pages = "401-407", doi = "10.1016/j.indcrop.2013.09.038" }
Dzamic, A. M., Soković, M., Novaković, M., Jadranin, M., Ristic, M. S., Tešević, V.,& Marin, P. D.. (2013). Composition, antifungal and antioxidant properties of Hyssopus officinalis L. subsp pilifer (Pant.) Murb. essential oil and deodorized extracts. in Industrial Crops and Products Elsevier., 51, 401-407. https://doi.org/10.1016/j.indcrop.2013.09.038
Dzamic AM, Soković M, Novaković M, Jadranin M, Ristic MS, Tešević V, Marin PD. Composition, antifungal and antioxidant properties of Hyssopus officinalis L. subsp pilifer (Pant.) Murb. essential oil and deodorized extracts. in Industrial Crops and Products. 2013;51:401-407. doi:10.1016/j.indcrop.2013.09.038 .
Dzamic, Ana M., Soković, Marina, Novaković, Miroslav, Jadranin, Milka, Ristic, Mihailo S., Tešević, Vele, Marin, Petar D., "Composition, antifungal and antioxidant properties of Hyssopus officinalis L. subsp pilifer (Pant.) Murb. essential oil and deodorized extracts" in Industrial Crops and Products, 51 (2013):401-407, https://doi.org/10.1016/j.indcrop.2013.09.038 . .