Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai
Produkcija i karakterizacija ramnolipida soja Pseudomonas aeruginosa san-ai
Abstract
The production and characteristics of rhamnolipid biosurfactant obtained by the strain Pseudomonas aeruginosa san-ai were investigated. With regard to the carbon and nitrogen sources, several media were tested to enhance the production of rhamnolipids. Phosphate-limited proteose peptone-ammonium salt (PPAS) medium supplemented with sunflower oil as a source of carbon and mineral ammonium chloride and peptone as nitrogen sources greatly improved the production of rhamnolipid, from 0.15 on basic PPAS (C/N ratio 4.0) to 3 g L-1 on optimized PPAS medium (C/N ratio 7.7). Response surface methodology analysis was used for testing the effect of three factors, i.e., temperature, concentration of carbon and nitrogen source (mass %), in the optimized PPAS medium on the production of rhamnolipid. The isolated rhamnolipids were characterized by infrared (IR) spectroscopy and electrospray ionization mass spectrometry (ESI-MS). The IR spectra confirmed that the isolated compound corresponded to the ...rhamnolipid structure, whereas MS indicated that the isolated preparation was a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic and di-rhamno-di-lipidic congeners.
U ovom radu je opisana produkcija i karakterizacija ramnolipidnog biosurfaktanta soja Pseudomonas aeruginosa san-ai. Ispitan je uticaj različitih izvora ugljenika i azota na produkciju ramnolipida. Podloga proteoze pepton-amonijum soli sa ograničenom količinom fosfata (PPAS) sa suncokretovim uljem kao izvorom ugljenika i amonijum-hloridom i peptonom kao izvorom azota, znatno povećava produkciju ramnolipida, od 0,15, na PPAS sa standardnim sastavom (odnos C/N 4,0), do 3 g L-1 na optimizovanoj PPAS podlozi (odnos C/N 7,7). Metodom planiranog eksperimenta (response surface methodology) ispitan je efekat tri parametra: temperature, koncentracije izvora ugljenika i azota na optimizovanoj podlozi. Karakterizacija izolovanog preparata ramnolipida urađena je pomoću IR i ESI-MS analiza. IR analiza je potvrdila prisustvo strukturnih elemenata karakterističnih za ramnolipide. MS analiza pokazala je da reč o smeši u kojoj su prisutne mono-ramno-monolipidne, di-ramno-mono-lipidne i di-ramno-di-lipi...dne komponente.
Keywords:
rhamnolipids / Pseudomonas aeruginosa / renewable sourcesSource:
Journal of the Serbian Chemical Society, 2012, 77, 1, 27-42Publisher:
- Serbian Chemical Society
Funding / projects:
DOI: 10.2298/JSC110211156R
ISSN: 0352-5139
WoS: 000300386100004
Scopus: 2-s2.0-84858652979
Collections
Institution/Community
IHTMTY - JOUR AU - Rikalović, Milena AU - Gojgić-Cvijović, Gordana AU - Vrvić, Miroslav AU - Karadžić, Ivanka PY - 2012 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/998 AB - The production and characteristics of rhamnolipid biosurfactant obtained by the strain Pseudomonas aeruginosa san-ai were investigated. With regard to the carbon and nitrogen sources, several media were tested to enhance the production of rhamnolipids. Phosphate-limited proteose peptone-ammonium salt (PPAS) medium supplemented with sunflower oil as a source of carbon and mineral ammonium chloride and peptone as nitrogen sources greatly improved the production of rhamnolipid, from 0.15 on basic PPAS (C/N ratio 4.0) to 3 g L-1 on optimized PPAS medium (C/N ratio 7.7). Response surface methodology analysis was used for testing the effect of three factors, i.e., temperature, concentration of carbon and nitrogen source (mass %), in the optimized PPAS medium on the production of rhamnolipid. The isolated rhamnolipids were characterized by infrared (IR) spectroscopy and electrospray ionization mass spectrometry (ESI-MS). The IR spectra confirmed that the isolated compound corresponded to the rhamnolipid structure, whereas MS indicated that the isolated preparation was a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic and di-rhamno-di-lipidic congeners. AB - U ovom radu je opisana produkcija i karakterizacija ramnolipidnog biosurfaktanta soja Pseudomonas aeruginosa san-ai. Ispitan je uticaj različitih izvora ugljenika i azota na produkciju ramnolipida. Podloga proteoze pepton-amonijum soli sa ograničenom količinom fosfata (PPAS) sa suncokretovim uljem kao izvorom ugljenika i amonijum-hloridom i peptonom kao izvorom azota, znatno povećava produkciju ramnolipida, od 0,15, na PPAS sa standardnim sastavom (odnos C/N 4,0), do 3 g L-1 na optimizovanoj PPAS podlozi (odnos C/N 7,7). Metodom planiranog eksperimenta (response surface methodology) ispitan je efekat tri parametra: temperature, koncentracije izvora ugljenika i azota na optimizovanoj podlozi. Karakterizacija izolovanog preparata ramnolipida urađena je pomoću IR i ESI-MS analiza. IR analiza je potvrdila prisustvo strukturnih elemenata karakterističnih za ramnolipide. MS analiza pokazala je da reč o smeši u kojoj su prisutne mono-ramno-monolipidne, di-ramno-mono-lipidne i di-ramno-di-lipidne komponente. PB - Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T1 - Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai T1 - Produkcija i karakterizacija ramnolipida soja Pseudomonas aeruginosa san-ai VL - 77 IS - 1 SP - 27 EP - 42 DO - 10.2298/JSC110211156R ER -
@article{ author = "Rikalović, Milena and Gojgić-Cvijović, Gordana and Vrvić, Miroslav and Karadžić, Ivanka", year = "2012", abstract = "The production and characteristics of rhamnolipid biosurfactant obtained by the strain Pseudomonas aeruginosa san-ai were investigated. With regard to the carbon and nitrogen sources, several media were tested to enhance the production of rhamnolipids. Phosphate-limited proteose peptone-ammonium salt (PPAS) medium supplemented with sunflower oil as a source of carbon and mineral ammonium chloride and peptone as nitrogen sources greatly improved the production of rhamnolipid, from 0.15 on basic PPAS (C/N ratio 4.0) to 3 g L-1 on optimized PPAS medium (C/N ratio 7.7). Response surface methodology analysis was used for testing the effect of three factors, i.e., temperature, concentration of carbon and nitrogen source (mass %), in the optimized PPAS medium on the production of rhamnolipid. The isolated rhamnolipids were characterized by infrared (IR) spectroscopy and electrospray ionization mass spectrometry (ESI-MS). The IR spectra confirmed that the isolated compound corresponded to the rhamnolipid structure, whereas MS indicated that the isolated preparation was a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic and di-rhamno-di-lipidic congeners., U ovom radu je opisana produkcija i karakterizacija ramnolipidnog biosurfaktanta soja Pseudomonas aeruginosa san-ai. Ispitan je uticaj različitih izvora ugljenika i azota na produkciju ramnolipida. Podloga proteoze pepton-amonijum soli sa ograničenom količinom fosfata (PPAS) sa suncokretovim uljem kao izvorom ugljenika i amonijum-hloridom i peptonom kao izvorom azota, znatno povećava produkciju ramnolipida, od 0,15, na PPAS sa standardnim sastavom (odnos C/N 4,0), do 3 g L-1 na optimizovanoj PPAS podlozi (odnos C/N 7,7). Metodom planiranog eksperimenta (response surface methodology) ispitan je efekat tri parametra: temperature, koncentracije izvora ugljenika i azota na optimizovanoj podlozi. Karakterizacija izolovanog preparata ramnolipida urađena je pomoću IR i ESI-MS analiza. IR analiza je potvrdila prisustvo strukturnih elemenata karakterističnih za ramnolipide. MS analiza pokazala je da reč o smeši u kojoj su prisutne mono-ramno-monolipidne, di-ramno-mono-lipidne i di-ramno-di-lipidne komponente.", publisher = "Serbian Chemical Society", journal = "Journal of the Serbian Chemical Society", title = "Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai, Produkcija i karakterizacija ramnolipida soja Pseudomonas aeruginosa san-ai", volume = "77", number = "1", pages = "27-42", doi = "10.2298/JSC110211156R" }
Rikalović, M., Gojgić-Cvijović, G., Vrvić, M.,& Karadžić, I.. (2012). Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai. in Journal of the Serbian Chemical Society Serbian Chemical Society., 77(1), 27-42. https://doi.org/10.2298/JSC110211156R
Rikalović M, Gojgić-Cvijović G, Vrvić M, Karadžić I. Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai. in Journal of the Serbian Chemical Society. 2012;77(1):27-42. doi:10.2298/JSC110211156R .
Rikalović, Milena, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, Karadžić, Ivanka, "Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai" in Journal of the Serbian Chemical Society, 77, no. 1 (2012):27-42, https://doi.org/10.2298/JSC110211156R . .