A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure
Glukan iz suvog aktivnog pekarskog kvasca (Saccharomyces cerevisiae) - hemijsko i enzimsko izučavanje strukture
Апстракт
The structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry baker’s yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry, NMR spectroscopy, and enzymic hydrolysis, as a new approach in determination of structures. The main structural feature of the polysaccharide deduced on the basis of the obtained results is a linear chain of (1→3)-linked β-D-glucopyranoses, a part of which is substituted through the positions O-6. The side units or groups are either a single D-glucopyranose or (1→3)-β-oligoglucosides, linked to the main chaing through (1→6)-glucosidic linkages. The low optical rotation as well as the 13C-NMR and FTIR spectra suggest that the glycosidic linkages are in the β-D-configuration.
Ispitivana je struktura homopolisaharida izolovanog iz suvog aktivnog pekarskog kvasca (Saccharomyces cerevisiae), metodom metilovanja perjodatnom oksidacijom, masenom spektrometrijom, NMR spektroskopijom i enzimskom hidrolizom, kao novim pristupom u izučavanju strukture polisaharida. Dobijeni rezultati ukazuju da polisaharid, koji se sastoji iz D-glukoze, ima (1→3)-D-glukopolimerni niz kao osnovni, a da su D-glukopiranozni ostaci vezani za osnovni niz preko položaja O-6 i to ili kao pojedinačne bočne jedinice ili kao deo oligomernih bočnih grupa koje čine (1→3)-D-glukopiranozil ostaci. Konfiguracija glikozidnih veza je β-D-tipa.
Кључне речи:
active dry baker’s yeast / Saccharomyces cerevisiae / polysaccharides / glucan / structure / chemical and enzymatic methodsИзвор:
Journal of the Serbian Chemical Society, 2003, 68, 11, 805-809Издавач:
- Serbian Chemical Society
DOI: 10.2298/JSC0311805Z
ISSN: 0352-5139
WoS: 000187394400003
Scopus: 2-s2.0-1442359740
Институција/група
IHTMTY - JOUR AU - Zlatković, Dragan AU - Jakovljević, Dragica AU - Zeković, Đorđe AU - Vrvić, Miroslav PY - 2003 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/95 AB - The structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry baker’s yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry, NMR spectroscopy, and enzymic hydrolysis, as a new approach in determination of structures. The main structural feature of the polysaccharide deduced on the basis of the obtained results is a linear chain of (1→3)-linked β-D-glucopyranoses, a part of which is substituted through the positions O-6. The side units or groups are either a single D-glucopyranose or (1→3)-β-oligoglucosides, linked to the main chaing through (1→6)-glucosidic linkages. The low optical rotation as well as the 13C-NMR and FTIR spectra suggest that the glycosidic linkages are in the β-D-configuration. AB - Ispitivana je struktura homopolisaharida izolovanog iz suvog aktivnog pekarskog kvasca (Saccharomyces cerevisiae), metodom metilovanja perjodatnom oksidacijom, masenom spektrometrijom, NMR spektroskopijom i enzimskom hidrolizom, kao novim pristupom u izučavanju strukture polisaharida. Dobijeni rezultati ukazuju da polisaharid, koji se sastoji iz D-glukoze, ima (1→3)-D-glukopolimerni niz kao osnovni, a da su D-glukopiranozni ostaci vezani za osnovni niz preko položaja O-6 i to ili kao pojedinačne bočne jedinice ili kao deo oligomernih bočnih grupa koje čine (1→3)-D-glukopiranozil ostaci. Konfiguracija glikozidnih veza je β-D-tipa. PB - Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T1 - A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure T1 - Glukan iz suvog aktivnog pekarskog kvasca (Saccharomyces cerevisiae) - hemijsko i enzimsko izučavanje strukture VL - 68 IS - 11 SP - 805 EP - 809 DO - 10.2298/JSC0311805Z ER -
@article{ author = "Zlatković, Dragan and Jakovljević, Dragica and Zeković, Đorđe and Vrvić, Miroslav", year = "2003", abstract = "The structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry baker’s yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry, NMR spectroscopy, and enzymic hydrolysis, as a new approach in determination of structures. The main structural feature of the polysaccharide deduced on the basis of the obtained results is a linear chain of (1→3)-linked β-D-glucopyranoses, a part of which is substituted through the positions O-6. The side units or groups are either a single D-glucopyranose or (1→3)-β-oligoglucosides, linked to the main chaing through (1→6)-glucosidic linkages. The low optical rotation as well as the 13C-NMR and FTIR spectra suggest that the glycosidic linkages are in the β-D-configuration., Ispitivana je struktura homopolisaharida izolovanog iz suvog aktivnog pekarskog kvasca (Saccharomyces cerevisiae), metodom metilovanja perjodatnom oksidacijom, masenom spektrometrijom, NMR spektroskopijom i enzimskom hidrolizom, kao novim pristupom u izučavanju strukture polisaharida. Dobijeni rezultati ukazuju da polisaharid, koji se sastoji iz D-glukoze, ima (1→3)-D-glukopolimerni niz kao osnovni, a da su D-glukopiranozni ostaci vezani za osnovni niz preko položaja O-6 i to ili kao pojedinačne bočne jedinice ili kao deo oligomernih bočnih grupa koje čine (1→3)-D-glukopiranozil ostaci. Konfiguracija glikozidnih veza je β-D-tipa.", publisher = "Serbian Chemical Society", journal = "Journal of the Serbian Chemical Society", title = "A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure, Glukan iz suvog aktivnog pekarskog kvasca (Saccharomyces cerevisiae) - hemijsko i enzimsko izučavanje strukture", volume = "68", number = "11", pages = "805-809", doi = "10.2298/JSC0311805Z" }
Zlatković, D., Jakovljević, D., Zeković, Đ.,& Vrvić, M.. (2003). A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure. in Journal of the Serbian Chemical Society Serbian Chemical Society., 68(11), 805-809. https://doi.org/10.2298/JSC0311805Z
Zlatković D, Jakovljević D, Zeković Đ, Vrvić M. A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure. in Journal of the Serbian Chemical Society. 2003;68(11):805-809. doi:10.2298/JSC0311805Z .
Zlatković, Dragan, Jakovljević, Dragica, Zeković, Đorđe, Vrvić, Miroslav, "A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure" in Journal of the Serbian Chemical Society, 68, no. 11 (2003):805-809, https://doi.org/10.2298/JSC0311805Z . .