Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae
2012
Autori
Pavlovic, R.Grujic, Marica
Dojnov, Biljana
Vujčić, Miroslava
Nenadovic, Vera
Ivanovic, Jelisaveta
Vujčić, Zoran
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven β-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and β-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-β-1,4-glucanase; subsequently, the obtained fragments are broken down by β-D-glucosidase mostly in middle midgut.
Ključne reči:
Cerambycidae / Cerambyx cerdo / cellulase / β-D-glucosidase / endocellulase / isoforms / zymogram / midgut / SerbiaIzvor:
Archives of Biological Sciences, 2012, 64, 2, 757-765Izdavač:
- Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
Finansiranje / projekti:
- Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-MESTD-Basic Research (BR or ON)-172048)
DOI: 10.2298/ABS1202757P
ISSN: 0354-4664
WoS: 000304339100043
Scopus: 2-s2.0-84862255096
Institucija/grupa
IHTMTY - JOUR AU - Pavlovic, R. AU - Grujic, Marica AU - Dojnov, Biljana AU - Vujčić, Miroslava AU - Nenadovic, Vera AU - Ivanovic, Jelisaveta AU - Vujčić, Zoran PY - 2012 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/943 AB - The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven β-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and β-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-β-1,4-glucanase; subsequently, the obtained fragments are broken down by β-D-glucosidase mostly in middle midgut. PB - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd T2 - Archives of Biological Sciences T1 - Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae VL - 64 IS - 2 SP - 757 EP - 765 DO - 10.2298/ABS1202757P ER -
@article{ author = "Pavlovic, R. and Grujic, Marica and Dojnov, Biljana and Vujčić, Miroslava and Nenadovic, Vera and Ivanovic, Jelisaveta and Vujčić, Zoran", year = "2012", abstract = "The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven β-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and β-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-β-1,4-glucanase; subsequently, the obtained fragments are broken down by β-D-glucosidase mostly in middle midgut.", publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd", journal = "Archives of Biological Sciences", title = "Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae", volume = "64", number = "2", pages = "757-765", doi = "10.2298/ABS1202757P" }
Pavlovic, R., Grujic, M., Dojnov, B., Vujčić, M., Nenadovic, V., Ivanovic, J.,& Vujčić, Z.. (2012). Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae. in Archives of Biological Sciences Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 64(2), 757-765. https://doi.org/10.2298/ABS1202757P
Pavlovic R, Grujic M, Dojnov B, Vujčić M, Nenadovic V, Ivanovic J, Vujčić Z. Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae. in Archives of Biological Sciences. 2012;64(2):757-765. doi:10.2298/ABS1202757P .
Pavlovic, R., Grujic, Marica, Dojnov, Biljana, Vujčić, Miroslava, Nenadovic, Vera, Ivanovic, Jelisaveta, Vujčić, Zoran, "Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae" in Archives of Biological Sciences, 64, no. 2 (2012):757-765, https://doi.org/10.2298/ABS1202757P . .