Приказ основних података о документу

dc.creatorBožić, Nataša
dc.creatorRuiz, Jordi
dc.creatorLopez-Santin, Josep
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:28:12Z
dc.date.available2019-01-30T17:28:12Z
dc.date.issued2011
dc.identifier.issn1369-703X
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/899
dc.description.abstractHighly efficient raw starch digesting alpha-amylase was produced after 24 h of batch fermentation of Bacillus licheniformis ATCC 9945a in laboratory bioreactor at 37 degrees C. The enzyme was purified by gel filtration chromatographies with 6-fold increase of specific activity and 38% recovery and showed a molecular mass of 31 kDa by SDS-PAGE. The purified enzyme had an optimum pH of 6.5 and optimum temperature of 90 degrees C. The purified alpha-amylase in the presence of CaCl(2) retained 55% of its activity after 6 h of incubation at 70 degrees C. Co(2+), Ni(2+) and Ca(2+) slightly stimulated, while Hg(2+) completely inhibited alpha-amylase activity. Hydrolysis rates of raw triticale, wheat, potato, horseradish and corn starches, at 1% concentration were 63, 60, 59, 52 and 37%, respectively, in a period of 4 h. The properties of the purified enzyme proved its high efficacy for digesting diverse raw starches below gelatinization temperature and, hence, its potential commercial value to use as an industrial enzyme.en
dc.publisherElsevier Science Sa, Lausanne
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.relationSpanish MICINN [CTQ2008-00578]
dc.rightsrestrictedAccess
dc.sourceBiochemical Engineering Journal
dc.subjectalpha-Amylaseen
dc.subjectBacillus licheniformisen
dc.subjectRaw starch hydrolysisen
dc.subjectThermostableen
dc.subjectPurificationen
dc.titleProduction and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945aen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРуиз, Јорди; Божић, Наташа; Вујциц, Зоран; Лопез-Сантин, Јосеп;
dc.citation.volume53
dc.citation.issue2
dc.citation.spage203
dc.citation.epage209
dc.citation.other53(2): 203-209
dc.citation.rankM21
dc.identifier.doi10.1016/j.bej.2010.10.014
dc.identifier.scopus2-s2.0-78649966240
dc.identifier.wos000287116300007
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу