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Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a
dc.creator | Božić, Nataša | |
dc.creator | Ruiz, Jordi | |
dc.creator | Lopez-Santin, Josep | |
dc.creator | Vujčić, Zoran | |
dc.date.accessioned | 2019-01-30T17:28:12Z | |
dc.date.available | 2019-01-30T17:28:12Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 1369-703X | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/899 | |
dc.description.abstract | Highly efficient raw starch digesting alpha-amylase was produced after 24 h of batch fermentation of Bacillus licheniformis ATCC 9945a in laboratory bioreactor at 37 degrees C. The enzyme was purified by gel filtration chromatographies with 6-fold increase of specific activity and 38% recovery and showed a molecular mass of 31 kDa by SDS-PAGE. The purified enzyme had an optimum pH of 6.5 and optimum temperature of 90 degrees C. The purified alpha-amylase in the presence of CaCl(2) retained 55% of its activity after 6 h of incubation at 70 degrees C. Co(2+), Ni(2+) and Ca(2+) slightly stimulated, while Hg(2+) completely inhibited alpha-amylase activity. Hydrolysis rates of raw triticale, wheat, potato, horseradish and corn starches, at 1% concentration were 63, 60, 59, 52 and 37%, respectively, in a period of 4 h. The properties of the purified enzyme proved its high efficacy for digesting diverse raw starches below gelatinization temperature and, hence, its potential commercial value to use as an industrial enzyme. | en |
dc.publisher | Elsevier Science Sa, Lausanne | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS// | |
dc.relation | Spanish MICINN [CTQ2008-00578] | |
dc.rights | restrictedAccess | |
dc.source | Biochemical Engineering Journal | |
dc.subject | alpha-Amylase | en |
dc.subject | Bacillus licheniformis | en |
dc.subject | Raw starch hydrolysis | en |
dc.subject | Thermostable | en |
dc.subject | Purification | en |
dc.title | Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Руиз, Јорди; Божић, Наташа; Вујциц, Зоран; Лопез-Сантин, Јосеп; | |
dc.citation.volume | 53 | |
dc.citation.issue | 2 | |
dc.citation.spage | 203 | |
dc.citation.epage | 209 | |
dc.citation.other | 53(2): 203-209 | |
dc.citation.rank | M21 | |
dc.identifier.doi | 10.1016/j.bej.2010.10.014 | |
dc.identifier.scopus | 2-s2.0-78649966240 | |
dc.identifier.wos | 000287116300007 | |
dc.type.version | publishedVersion |