Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization
Streptomyces subtilizinu sličan inhibitor (SSL-inhibitor) iz streptomyces hygroscopicus CH-7 - detekcija i delimična karakterizacija
Апстракт
Strain Streptomyces (S.) hygroscopicus CH-7 during the fermentation of polyketide antibiotic produces as a by product proteolytic complex with significant trypsin like activity. Also, S. hygroscopicus CH-7 produces extracellulary proteinaceous protease inhibitor known as Streptomyces subtilisine like inhibitor (SSL-inhibitor). Detection of SSL-inhibitor is preformed on the base of stability of SSL-inhibitors on very low pH (final concentracion of TCA is 9%). Molecular mass of SSL-inhibitor is approximately 11 kDa, and it provides very wide range of inhibitory activity toward different serin proteases: trypsin, protease from Bacillus (B.) subtilis, proteinase K from Tritirachium (T.) album, protease from B. licheniformis, protease from S. griseus and the complex of proteases from S. hygroscopicus CH-7.
Soj S. hygroscopicus CH-7 u toku fermentacije ekstracelularno proizvodi proteinski inhibitor proteaza koji pripada grupi Streptomyces subtilizinu sličnim inhibitorima (Streptomyces subtilisine like inhibitor: SSL-inhibitor). Postupak za detekciju je jednostavan i bazira se na činjenici da su SSL-inhibitori stabilni na veoma niskim pH. SSL-inhibitor iz S. hygroscopicus CH-7 pokazuje širok spektar inhibitornog dejstva na: tripsin, proteazu iz B. subtilis, proteinazu K iz T. album, proteazu iz B. licheniformis, proteazu iz S. griseus i na sirovi proteoliticki kompleks S. hygroscopicus CH-7. Molekulska masa SSL-inhibitora određena gel filtracijom iznosi 11 kDa, slično većini do sada izolovanih SSL.
Кључне речи:
SSL-inhibitor / Strepromyces hygroscopicus CH-7 / production / detection / partial characterizationИзвор:
Acta biologica iugoslavica - serija B: Mikrobiologija, 2000, 37, 1, 31-36Институција/група
IHTMTY - JOUR AU - Izrael, Lidija AU - Karadžić, Ivanka AU - Gojgić-Cvijović, Gordana AU - Vrvić, Miroslav PY - 2000 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/8 AB - Strain Streptomyces (S.) hygroscopicus CH-7 during the fermentation of polyketide antibiotic produces as a by product proteolytic complex with significant trypsin like activity. Also, S. hygroscopicus CH-7 produces extracellulary proteinaceous protease inhibitor known as Streptomyces subtilisine like inhibitor (SSL-inhibitor). Detection of SSL-inhibitor is preformed on the base of stability of SSL-inhibitors on very low pH (final concentracion of TCA is 9%). Molecular mass of SSL-inhibitor is approximately 11 kDa, and it provides very wide range of inhibitory activity toward different serin proteases: trypsin, protease from Bacillus (B.) subtilis, proteinase K from Tritirachium (T.) album, protease from B. licheniformis, protease from S. griseus and the complex of proteases from S. hygroscopicus CH-7. AB - Soj S. hygroscopicus CH-7 u toku fermentacije ekstracelularno proizvodi proteinski inhibitor proteaza koji pripada grupi Streptomyces subtilizinu sličnim inhibitorima (Streptomyces subtilisine like inhibitor: SSL-inhibitor). Postupak za detekciju je jednostavan i bazira se na činjenici da su SSL-inhibitori stabilni na veoma niskim pH. SSL-inhibitor iz S. hygroscopicus CH-7 pokazuje širok spektar inhibitornog dejstva na: tripsin, proteazu iz B. subtilis, proteinazu K iz T. album, proteazu iz B. licheniformis, proteazu iz S. griseus i na sirovi proteoliticki kompleks S. hygroscopicus CH-7. Molekulska masa SSL-inhibitora određena gel filtracijom iznosi 11 kDa, slično većini do sada izolovanih SSL. T2 - Acta biologica iugoslavica - serija B: Mikrobiologija T1 - Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization T1 - Streptomyces subtilizinu sličan inhibitor (SSL-inhibitor) iz streptomyces hygroscopicus CH-7 - detekcija i delimična karakterizacija VL - 37 IS - 1 SP - 31 EP - 36 UR - https://hdl.handle.net/21.15107/rcub_cer_8 ER -
@article{ author = "Izrael, Lidija and Karadžić, Ivanka and Gojgić-Cvijović, Gordana and Vrvić, Miroslav", year = "2000", abstract = "Strain Streptomyces (S.) hygroscopicus CH-7 during the fermentation of polyketide antibiotic produces as a by product proteolytic complex with significant trypsin like activity. Also, S. hygroscopicus CH-7 produces extracellulary proteinaceous protease inhibitor known as Streptomyces subtilisine like inhibitor (SSL-inhibitor). Detection of SSL-inhibitor is preformed on the base of stability of SSL-inhibitors on very low pH (final concentracion of TCA is 9%). Molecular mass of SSL-inhibitor is approximately 11 kDa, and it provides very wide range of inhibitory activity toward different serin proteases: trypsin, protease from Bacillus (B.) subtilis, proteinase K from Tritirachium (T.) album, protease from B. licheniformis, protease from S. griseus and the complex of proteases from S. hygroscopicus CH-7., Soj S. hygroscopicus CH-7 u toku fermentacije ekstracelularno proizvodi proteinski inhibitor proteaza koji pripada grupi Streptomyces subtilizinu sličnim inhibitorima (Streptomyces subtilisine like inhibitor: SSL-inhibitor). Postupak za detekciju je jednostavan i bazira se na činjenici da su SSL-inhibitori stabilni na veoma niskim pH. SSL-inhibitor iz S. hygroscopicus CH-7 pokazuje širok spektar inhibitornog dejstva na: tripsin, proteazu iz B. subtilis, proteinazu K iz T. album, proteazu iz B. licheniformis, proteazu iz S. griseus i na sirovi proteoliticki kompleks S. hygroscopicus CH-7. Molekulska masa SSL-inhibitora određena gel filtracijom iznosi 11 kDa, slično većini do sada izolovanih SSL.", journal = "Acta biologica iugoslavica - serija B: Mikrobiologija", title = "Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization, Streptomyces subtilizinu sličan inhibitor (SSL-inhibitor) iz streptomyces hygroscopicus CH-7 - detekcija i delimična karakterizacija", volume = "37", number = "1", pages = "31-36", url = "https://hdl.handle.net/21.15107/rcub_cer_8" }
Izrael, L., Karadžić, I., Gojgić-Cvijović, G.,& Vrvić, M.. (2000). Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization. in Acta biologica iugoslavica - serija B: Mikrobiologija, 37(1), 31-36. https://hdl.handle.net/21.15107/rcub_cer_8
Izrael L, Karadžić I, Gojgić-Cvijović G, Vrvić M. Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization. in Acta biologica iugoslavica - serija B: Mikrobiologija. 2000;37(1):31-36. https://hdl.handle.net/21.15107/rcub_cer_8 .
Izrael, Lidija, Karadžić, Ivanka, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, "Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization" in Acta biologica iugoslavica - serija B: Mikrobiologija, 37, no. 1 (2000):31-36, https://hdl.handle.net/21.15107/rcub_cer_8 .