A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide
Samo za registrovane korisnike
2011
Autori
Vujčić, MiroslavaLazić, Milan
Milenković, Milica R.
Sladić, Dušan
Radulovic, Sinisa
Filipovic, Nenad
Anđelković, Katarina
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Organometallic Cd(II) compounds have recently attracted attention for their anticancer activity. The interaction of the dinuclear complex of Cd(II) with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N',N'(2)-bis[(1E)-1-(2pyridyl) ethylidene] propanedihydrazide (Cd(II)H(2)L), with calf thymus DNA (CT-DNA) was monitored by blue shift in UV-vis spectra of the complex. The binding constant of Cd(II) H2L complex with CT-DNA was determined (K(B) = 1.8 x 10(4) M(-1)) and was indicative of minor groove binding. Agarose gel electrophoretic changes in mobility of supercoiled and circular forms of pBR322 and pUC18 plasmids in the presence of the complex suggest that conformational changes in the plasmids occur upon binding of the Cd(II) H2L complex. The Cd(II) H2L complex induced perturbation of the cell cycle phase distribution and an increase in the percentage of cells in the sub-G1 phase of human cervical cancer HeLa cell line and murine melanoma B16 cell line. Imm...unoblotting analysis showed the overexpression of Bcl-2 protein with the Cd(II)H(2)L complex.
Ključne reči:
DNA Binding / Electrophoretic Analysis / Cell Cycle Phase PerturbationIzvor:
Journal of Biochemical and Molecular Toxicology, 2011, 25, 3, 175-182Izdavač:
- Wiley-Blackwell, Malden
Finansiranje / projekti:
- Interakcije prirodnih proizvoda i njihovih analoga sa proteinima i nukleinskim kiselinama (RS-MESTD-MPN2006-2010-142026)
- Farmakodinamska i farmakogenetska istraživanja novih lekova i prediktivna/prognostička vrednost farmakoterapije u onkologiji (RS-MESTD-MPN2006-2010-145035)
DOI: 10.1002/jbt.20374
ISSN: 1095-6670
PubMed: 21671309
WoS: 000291562100007
Scopus: 2-s2.0-79958856469
Institucija/grupa
IHTMTY - JOUR AU - Vujčić, Miroslava AU - Lazić, Milan AU - Milenković, Milica R. AU - Sladić, Dušan AU - Radulovic, Sinisa AU - Filipovic, Nenad AU - Anđelković, Katarina PY - 2011 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/784 AB - Organometallic Cd(II) compounds have recently attracted attention for their anticancer activity. The interaction of the dinuclear complex of Cd(II) with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N',N'(2)-bis[(1E)-1-(2pyridyl) ethylidene] propanedihydrazide (Cd(II)H(2)L), with calf thymus DNA (CT-DNA) was monitored by blue shift in UV-vis spectra of the complex. The binding constant of Cd(II) H2L complex with CT-DNA was determined (K(B) = 1.8 x 10(4) M(-1)) and was indicative of minor groove binding. Agarose gel electrophoretic changes in mobility of supercoiled and circular forms of pBR322 and pUC18 plasmids in the presence of the complex suggest that conformational changes in the plasmids occur upon binding of the Cd(II) H2L complex. The Cd(II) H2L complex induced perturbation of the cell cycle phase distribution and an increase in the percentage of cells in the sub-G1 phase of human cervical cancer HeLa cell line and murine melanoma B16 cell line. Immunoblotting analysis showed the overexpression of Bcl-2 protein with the Cd(II)H(2)L complex. PB - Wiley-Blackwell, Malden T2 - Journal of Biochemical and Molecular Toxicology T1 - A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide VL - 25 IS - 3 SP - 175 EP - 182 DO - 10.1002/jbt.20374 ER -
@article{ author = "Vujčić, Miroslava and Lazić, Milan and Milenković, Milica R. and Sladić, Dušan and Radulovic, Sinisa and Filipovic, Nenad and Anđelković, Katarina", year = "2011", abstract = "Organometallic Cd(II) compounds have recently attracted attention for their anticancer activity. The interaction of the dinuclear complex of Cd(II) with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N',N'(2)-bis[(1E)-1-(2pyridyl) ethylidene] propanedihydrazide (Cd(II)H(2)L), with calf thymus DNA (CT-DNA) was monitored by blue shift in UV-vis spectra of the complex. The binding constant of Cd(II) H2L complex with CT-DNA was determined (K(B) = 1.8 x 10(4) M(-1)) and was indicative of minor groove binding. Agarose gel electrophoretic changes in mobility of supercoiled and circular forms of pBR322 and pUC18 plasmids in the presence of the complex suggest that conformational changes in the plasmids occur upon binding of the Cd(II) H2L complex. The Cd(II) H2L complex induced perturbation of the cell cycle phase distribution and an increase in the percentage of cells in the sub-G1 phase of human cervical cancer HeLa cell line and murine melanoma B16 cell line. Immunoblotting analysis showed the overexpression of Bcl-2 protein with the Cd(II)H(2)L complex.", publisher = "Wiley-Blackwell, Malden", journal = "Journal of Biochemical and Molecular Toxicology", title = "A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide", volume = "25", number = "3", pages = "175-182", doi = "10.1002/jbt.20374" }
Vujčić, M., Lazić, M., Milenković, M. R., Sladić, D., Radulovic, S., Filipovic, N.,& Anđelković, K.. (2011). A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide. in Journal of Biochemical and Molecular Toxicology Wiley-Blackwell, Malden., 25(3), 175-182. https://doi.org/10.1002/jbt.20374
Vujčić M, Lazić M, Milenković MR, Sladić D, Radulovic S, Filipovic N, Anđelković K. A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide. in Journal of Biochemical and Molecular Toxicology. 2011;25(3):175-182. doi:10.1002/jbt.20374 .
Vujčić, Miroslava, Lazić, Milan, Milenković, Milica R., Sladić, Dušan, Radulovic, Sinisa, Filipovic, Nenad, Anđelković, Katarina, "A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide" in Journal of Biochemical and Molecular Toxicology, 25, no. 3 (2011):175-182, https://doi.org/10.1002/jbt.20374 . .