Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction
2024
Преузимање 🢃
Аутори
Stojanović, SanjaRistović, Marina
Margetić, Aleksandra
Božić, Nataša
Vujčić, Zoran
Dojnov, Biljana
Vujčić, Miroslava
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Recently, the exploration of agricultural by-products for biotechnological applications has gained substantial interest. This study addresses the multiple potential of corn agricultural waste, focusing on its dual utility: cellulase production and enhanced polyphenols extraction.
Aspergillus welwitschiae FAW1 and Aspergillus tubingensis FAT36 strains, isolated from the environment, has proven to be good producers of the cellulase enzyme complex and at the same time non-toxigenic and safe for use in food production. Due to the fact that most of the fungal enzymes are inducible, the substrates used in this solid-state fermentation were agro-waste materials rich
in cellulose and hemicellulose (corn silk and corn cob). The cellulase complex was produced by growing fungal strains at 28℃for 96 h.
Through enzyme assisted extraction, the release of polyphenols from corn silk was enhanced. The obtained extracts, whether we used FAW1 or FAT36 strain`s cellulase enzyme complex showed an increa...se in total phenolic content, ranging from 876 mg/mL to 3050 mg/mL and 2944 mg/mL, respectively, which was determined by the Folin Ciocalteu method. Increasing the amount of extracted
polyphenols led to the two to three times higher antioxidant activity of the extracts, according to ABTS and FRAP methods.
This enzymatic approach offers a sustainable and eco-friendly alternative to conventional extraction methods, mitigating environmental impact while enhancing product purity. These findings not only contribute to the valorization of agricultural waste, but also emphasize the potential of corn agro-waste
as a versatile resource for bioprocessing applications, connecting enzyme production, natural product extraction and added value products obtaining.
Кључне речи:
Corn agro-waste / waste valorization / cellulase / polyphenol extractionИзвор:
V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye, 2024, PP17-Издавач:
- Izmir Institute of Technology
Финансирање / пројекти:
- UniCorn - Unique enzyme solutions for obtaining addedvalue products from corn (RS-ScienceFundRS-Prizma2023_PM-7347)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
Институција/група
IHTMTY - CONF AU - Stojanović, Sanja AU - Ristović, Marina AU - Margetić, Aleksandra AU - Božić, Nataša AU - Vujčić, Zoran AU - Dojnov, Biljana AU - Vujčić, Miroslava PY - 2024 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7812 AB - Recently, the exploration of agricultural by-products for biotechnological applications has gained substantial interest. This study addresses the multiple potential of corn agricultural waste, focusing on its dual utility: cellulase production and enhanced polyphenols extraction. Aspergillus welwitschiae FAW1 and Aspergillus tubingensis FAT36 strains, isolated from the environment, has proven to be good producers of the cellulase enzyme complex and at the same time non-toxigenic and safe for use in food production. Due to the fact that most of the fungal enzymes are inducible, the substrates used in this solid-state fermentation were agro-waste materials rich in cellulose and hemicellulose (corn silk and corn cob). The cellulase complex was produced by growing fungal strains at 28℃for 96 h. Through enzyme assisted extraction, the release of polyphenols from corn silk was enhanced. The obtained extracts, whether we used FAW1 or FAT36 strain`s cellulase enzyme complex showed an increase in total phenolic content, ranging from 876 mg/mL to 3050 mg/mL and 2944 mg/mL, respectively, which was determined by the Folin Ciocalteu method. Increasing the amount of extracted polyphenols led to the two to three times higher antioxidant activity of the extracts, according to ABTS and FRAP methods. This enzymatic approach offers a sustainable and eco-friendly alternative to conventional extraction methods, mitigating environmental impact while enhancing product purity. These findings not only contribute to the valorization of agricultural waste, but also emphasize the potential of corn agro-waste as a versatile resource for bioprocessing applications, connecting enzyme production, natural product extraction and added value products obtaining. PB - Izmir Institute of Technology C3 - V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye T1 - Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction SP - PP17 UR - https://hdl.handle.net/21.15107/rcub_cer_7812 ER -
@conference{ author = "Stojanović, Sanja and Ristović, Marina and Margetić, Aleksandra and Božić, Nataša and Vujčić, Zoran and Dojnov, Biljana and Vujčić, Miroslava", year = "2024", abstract = "Recently, the exploration of agricultural by-products for biotechnological applications has gained substantial interest. This study addresses the multiple potential of corn agricultural waste, focusing on its dual utility: cellulase production and enhanced polyphenols extraction. Aspergillus welwitschiae FAW1 and Aspergillus tubingensis FAT36 strains, isolated from the environment, has proven to be good producers of the cellulase enzyme complex and at the same time non-toxigenic and safe for use in food production. Due to the fact that most of the fungal enzymes are inducible, the substrates used in this solid-state fermentation were agro-waste materials rich in cellulose and hemicellulose (corn silk and corn cob). The cellulase complex was produced by growing fungal strains at 28℃for 96 h. Through enzyme assisted extraction, the release of polyphenols from corn silk was enhanced. The obtained extracts, whether we used FAW1 or FAT36 strain`s cellulase enzyme complex showed an increase in total phenolic content, ranging from 876 mg/mL to 3050 mg/mL and 2944 mg/mL, respectively, which was determined by the Folin Ciocalteu method. Increasing the amount of extracted polyphenols led to the two to three times higher antioxidant activity of the extracts, according to ABTS and FRAP methods. This enzymatic approach offers a sustainable and eco-friendly alternative to conventional extraction methods, mitigating environmental impact while enhancing product purity. These findings not only contribute to the valorization of agricultural waste, but also emphasize the potential of corn agro-waste as a versatile resource for bioprocessing applications, connecting enzyme production, natural product extraction and added value products obtaining.", publisher = "Izmir Institute of Technology", journal = "V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye", title = "Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction", pages = "PP17", url = "https://hdl.handle.net/21.15107/rcub_cer_7812" }
Stojanović, S., Ristović, M., Margetić, A., Božić, N., Vujčić, Z., Dojnov, B.,& Vujčić, M.. (2024). Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction. in V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye Izmir Institute of Technology., PP17. https://hdl.handle.net/21.15107/rcub_cer_7812
Stojanović S, Ristović M, Margetić A, Božić N, Vujčić Z, Dojnov B, Vujčić M. Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction. in V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye. 2024;:PP17. https://hdl.handle.net/21.15107/rcub_cer_7812 .
Stojanović, Sanja, Ristović, Marina, Margetić, Aleksandra, Božić, Nataša, Vujčić, Zoran, Dojnov, Biljana, Vujčić, Miroslava, "Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction" in V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye (2024):PP17, https://hdl.handle.net/21.15107/rcub_cer_7812 .