Anodization/anaphoretic deposition of composite zein/hydroxyapatite coatings on titanium substrate
2024
Аутори
Božić, KatarinaPavlović, Miroslav
Kojić, Vesna V.
Veljović, Đorđe
Pantović Pavlović, Marijana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
INTRODUCTION: Hydroxyapatite, the main inorganic component of human bone, is a widely used bioceramic for bone implant coatings due to its chemical and structural similarity with bone minerals and approved biocompatibility. Nowadays, the surface properties of implants are also modified by the addition of different biopolymers. Features such as biocompatibility, tuned biodegradability, and non-cytotoxicity make these materials excellent candidates for these applications. Zein is one of the natural polymers that has gained great interest for biomedical applications due to its natural renewable resource, biodegradability, biocompatibility and potential antibacterial activity.
EXPERIMENTAL: In this study, zein/titanium dioxide (zein/TiO2), hydroxyapatite/zein/titanium dioxide (HAp/zein/TiO2) and strontium-doped hydroxyapatite/zein/titanium dioxide (Sr-HAp/zein/TiO2) coatings were obtained on a titanium substrate by in situ anodization/anaphoretic deposition method at a constant voltage of... 60 V and deposition time of 1 min. For the fabrication of HAp/zein/TiO2 and Sr-HAp/zein/TiO2 composite coatings, HAp and Sr-HAp nano-sized powders obtained by a modified chemical precipitation method were used. The microstructure and morphology of all coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Adhesion strength was measured according to the ASTM D3359-02 standard. Morphology and cell adhesion were analyzed by SEM.
RESULTS AND DISCUSSION: The advantage of this methodological approach compared to cataphoretic deposition is reflected in the simultaneous performance of several processes. The first is the anodization of the substrate surface, whereby a passive oxide layer (TiO2) is formed on the surface, which changes the structure and increases the surface roughness in a controlled manner. Another parallel process is the deposition of the HAp based coatings on the substrate. This way of coating formation shows better results than cataphoretic deposition in terms of better coating adhesion to the substrate. Adhesion strength was significantly improved compared to coatings obtained by cataphoretic processes, without the need for subsequent treatment. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy human lung fibroblasts (MRC-5) cells exposed to the obtained coatings.
CONCLUSIONS: Altogether, zein/TiO2, HAp/zein/TiO2 and Sr-HAp/zein/TiO2 could be promising non-toxic biomaterials for orthopedic and dental applications.
Кључне речи:
in situ synthesis / biomedical application / cytotoxicity / adhesionИзвор:
Chemical Industry - Supplementary Issue, 2024, 78, 1S, 42-42Издавач:
- Savez hemijskih inženjera Srbije
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
Напомена:
- Abstract from The ExcellMater Conference 2024: Innovative Biomaterials for Novel Medical Devices which was organized in Belgrade, Serbia, on April 10-12, 2024
Повезане информације:
Институција/група
IHTMTY - CONF AU - Božić, Katarina AU - Pavlović, Miroslav AU - Kojić, Vesna V. AU - Veljović, Đorđe AU - Pantović Pavlović, Marijana PY - 2024 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7655 AB - INTRODUCTION: Hydroxyapatite, the main inorganic component of human bone, is a widely used bioceramic for bone implant coatings due to its chemical and structural similarity with bone minerals and approved biocompatibility. Nowadays, the surface properties of implants are also modified by the addition of different biopolymers. Features such as biocompatibility, tuned biodegradability, and non-cytotoxicity make these materials excellent candidates for these applications. Zein is one of the natural polymers that has gained great interest for biomedical applications due to its natural renewable resource, biodegradability, biocompatibility and potential antibacterial activity. EXPERIMENTAL: In this study, zein/titanium dioxide (zein/TiO2), hydroxyapatite/zein/titanium dioxide (HAp/zein/TiO2) and strontium-doped hydroxyapatite/zein/titanium dioxide (Sr-HAp/zein/TiO2) coatings were obtained on a titanium substrate by in situ anodization/anaphoretic deposition method at a constant voltage of 60 V and deposition time of 1 min. For the fabrication of HAp/zein/TiO2 and Sr-HAp/zein/TiO2 composite coatings, HAp and Sr-HAp nano-sized powders obtained by a modified chemical precipitation method were used. The microstructure and morphology of all coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Adhesion strength was measured according to the ASTM D3359-02 standard. Morphology and cell adhesion were analyzed by SEM. RESULTS AND DISCUSSION: The advantage of this methodological approach compared to cataphoretic deposition is reflected in the simultaneous performance of several processes. The first is the anodization of the substrate surface, whereby a passive oxide layer (TiO2) is formed on the surface, which changes the structure and increases the surface roughness in a controlled manner. Another parallel process is the deposition of the HAp based coatings on the substrate. This way of coating formation shows better results than cataphoretic deposition in terms of better coating adhesion to the substrate. Adhesion strength was significantly improved compared to coatings obtained by cataphoretic processes, without the need for subsequent treatment. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy human lung fibroblasts (MRC-5) cells exposed to the obtained coatings. CONCLUSIONS: Altogether, zein/TiO2, HAp/zein/TiO2 and Sr-HAp/zein/TiO2 could be promising non-toxic biomaterials for orthopedic and dental applications. PB - Savez hemijskih inženjera Srbije C3 - Chemical Industry - Supplementary Issue T1 - Anodization/anaphoretic deposition of composite zein/hydroxyapatite coatings on titanium substrate VL - 78 IS - 1S SP - 42 EP - 42 UR - https://hdl.handle.net/21.15107/rcub_cer_7655 ER -
@conference{ author = "Božić, Katarina and Pavlović, Miroslav and Kojić, Vesna V. and Veljović, Đorđe and Pantović Pavlović, Marijana", year = "2024", abstract = "INTRODUCTION: Hydroxyapatite, the main inorganic component of human bone, is a widely used bioceramic for bone implant coatings due to its chemical and structural similarity with bone minerals and approved biocompatibility. Nowadays, the surface properties of implants are also modified by the addition of different biopolymers. Features such as biocompatibility, tuned biodegradability, and non-cytotoxicity make these materials excellent candidates for these applications. Zein is one of the natural polymers that has gained great interest for biomedical applications due to its natural renewable resource, biodegradability, biocompatibility and potential antibacterial activity. EXPERIMENTAL: In this study, zein/titanium dioxide (zein/TiO2), hydroxyapatite/zein/titanium dioxide (HAp/zein/TiO2) and strontium-doped hydroxyapatite/zein/titanium dioxide (Sr-HAp/zein/TiO2) coatings were obtained on a titanium substrate by in situ anodization/anaphoretic deposition method at a constant voltage of 60 V and deposition time of 1 min. For the fabrication of HAp/zein/TiO2 and Sr-HAp/zein/TiO2 composite coatings, HAp and Sr-HAp nano-sized powders obtained by a modified chemical precipitation method were used. The microstructure and morphology of all coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Adhesion strength was measured according to the ASTM D3359-02 standard. Morphology and cell adhesion were analyzed by SEM. RESULTS AND DISCUSSION: The advantage of this methodological approach compared to cataphoretic deposition is reflected in the simultaneous performance of several processes. The first is the anodization of the substrate surface, whereby a passive oxide layer (TiO2) is formed on the surface, which changes the structure and increases the surface roughness in a controlled manner. Another parallel process is the deposition of the HAp based coatings on the substrate. This way of coating formation shows better results than cataphoretic deposition in terms of better coating adhesion to the substrate. Adhesion strength was significantly improved compared to coatings obtained by cataphoretic processes, without the need for subsequent treatment. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy human lung fibroblasts (MRC-5) cells exposed to the obtained coatings. CONCLUSIONS: Altogether, zein/TiO2, HAp/zein/TiO2 and Sr-HAp/zein/TiO2 could be promising non-toxic biomaterials for orthopedic and dental applications.", publisher = "Savez hemijskih inženjera Srbije", journal = "Chemical Industry - Supplementary Issue", title = "Anodization/anaphoretic deposition of composite zein/hydroxyapatite coatings on titanium substrate", volume = "78", number = "1S", pages = "42-42", url = "https://hdl.handle.net/21.15107/rcub_cer_7655" }
Božić, K., Pavlović, M., Kojić, V. V., Veljović, Đ.,& Pantović Pavlović, M.. (2024). Anodization/anaphoretic deposition of composite zein/hydroxyapatite coatings on titanium substrate. in Chemical Industry - Supplementary Issue Savez hemijskih inženjera Srbije., 78(1S), 42-42. https://hdl.handle.net/21.15107/rcub_cer_7655
Božić K, Pavlović M, Kojić VV, Veljović Đ, Pantović Pavlović M. Anodization/anaphoretic deposition of composite zein/hydroxyapatite coatings on titanium substrate. in Chemical Industry - Supplementary Issue. 2024;78(1S):42-42. https://hdl.handle.net/21.15107/rcub_cer_7655 .
Božić, Katarina, Pavlović, Miroslav, Kojić, Vesna V., Veljović, Đorđe, Pantović Pavlović, Marijana, "Anodization/anaphoretic deposition of composite zein/hydroxyapatite coatings on titanium substrate" in Chemical Industry - Supplementary Issue, 78, no. 1S (2024):42-42, https://hdl.handle.net/21.15107/rcub_cer_7655 .