Every Drop Counts - The Current Methods for Determining the Quality of Human Milk
Аутори
Lugonja, NikoletaStanković, Dalibor
Marinković, Vesna
Avdalović, Jelena
Spasić, Snežana
Vrvić, Miroslav
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background and objectives: Human milk is the golden standard in infant nutrition. A very important segment of nutritional support for premature children is milk banks. In milk banks, during the process of storage and pasteurization, the characteristics of milk change, and consequently, its quality changes as well. Current electrochemical techniques (green analytical chemistry) can be used to determine the quality of milk regarding its total antioxidant capacity (TAC). Methods: The application of various electrochemical methods, such as differential pulse voltammetry, cyclic voltammetry, polarography, or potentiometry, enables monitoring of the quality and redox capacity of human milk and infant food. Changes in the TAC of human milk were examined after thermal treatments, which are commonly used in milk banks, as well as after supplementation with a fortifier. Results: The results obtained using electrochemical methods were compared to the most commonly used spectrophotometric methods ...for monitoring total antioxidant capacity. The obtained results showed that electrochemical methods can overcome the limitations of spectrophotometric methods, such as lower sensitivity, slower reaction and insensitivity at higher antioxidant concentrations. Cyclic and differential pulse voltammetry methods can be successfully applied as fast methods for testing milk quality in milk banks and clinical studies. Conclusion: The tested electrochemical methods are fast, cheap, and reliable in determining TAC since they are based on direct measurement of electron-donating components of milk and enable the quantitative determination of TAC in human milk and infant food. They are very important for routine and daily determinations of TAC in infant food, as well as for the control of milk freshness, especially after using fortifiers in neonatal units.
Кључне речи:
human milk / premature children / milk bank / total antioxidant capacity / electrochemical methodsИзвор:
Proceedings, 2023, 91, 1Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
Напомена:
- Proceedings of The 14th European Nutrition Conference FENS 2023, Belgrade, Serbia, 14–17 November 2023
Институција/група
IHTMTY - CONF AU - Lugonja, Nikoleta AU - Stanković, Dalibor AU - Marinković, Vesna AU - Avdalović, Jelena AU - Spasić, Snežana AU - Vrvić, Miroslav PY - 2023 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7460 AB - Background and objectives: Human milk is the golden standard in infant nutrition. A very important segment of nutritional support for premature children is milk banks. In milk banks, during the process of storage and pasteurization, the characteristics of milk change, and consequently, its quality changes as well. Current electrochemical techniques (green analytical chemistry) can be used to determine the quality of milk regarding its total antioxidant capacity (TAC). Methods: The application of various electrochemical methods, such as differential pulse voltammetry, cyclic voltammetry, polarography, or potentiometry, enables monitoring of the quality and redox capacity of human milk and infant food. Changes in the TAC of human milk were examined after thermal treatments, which are commonly used in milk banks, as well as after supplementation with a fortifier. Results: The results obtained using electrochemical methods were compared to the most commonly used spectrophotometric methods for monitoring total antioxidant capacity. The obtained results showed that electrochemical methods can overcome the limitations of spectrophotometric methods, such as lower sensitivity, slower reaction and insensitivity at higher antioxidant concentrations. Cyclic and differential pulse voltammetry methods can be successfully applied as fast methods for testing milk quality in milk banks and clinical studies. Conclusion: The tested electrochemical methods are fast, cheap, and reliable in determining TAC since they are based on direct measurement of electron-donating components of milk and enable the quantitative determination of TAC in human milk and infant food. They are very important for routine and daily determinations of TAC in infant food, as well as for the control of milk freshness, especially after using fortifiers in neonatal units. PB - MDPI C3 - Proceedings T1 - Every Drop Counts - The Current Methods for Determining the Quality of Human Milk VL - 91 IS - 1 DO - 10.3390/proceedings2023091314 ER -
@conference{ author = "Lugonja, Nikoleta and Stanković, Dalibor and Marinković, Vesna and Avdalović, Jelena and Spasić, Snežana and Vrvić, Miroslav", year = "2023", abstract = "Background and objectives: Human milk is the golden standard in infant nutrition. A very important segment of nutritional support for premature children is milk banks. In milk banks, during the process of storage and pasteurization, the characteristics of milk change, and consequently, its quality changes as well. Current electrochemical techniques (green analytical chemistry) can be used to determine the quality of milk regarding its total antioxidant capacity (TAC). Methods: The application of various electrochemical methods, such as differential pulse voltammetry, cyclic voltammetry, polarography, or potentiometry, enables monitoring of the quality and redox capacity of human milk and infant food. Changes in the TAC of human milk were examined after thermal treatments, which are commonly used in milk banks, as well as after supplementation with a fortifier. Results: The results obtained using electrochemical methods were compared to the most commonly used spectrophotometric methods for monitoring total antioxidant capacity. The obtained results showed that electrochemical methods can overcome the limitations of spectrophotometric methods, such as lower sensitivity, slower reaction and insensitivity at higher antioxidant concentrations. Cyclic and differential pulse voltammetry methods can be successfully applied as fast methods for testing milk quality in milk banks and clinical studies. Conclusion: The tested electrochemical methods are fast, cheap, and reliable in determining TAC since they are based on direct measurement of electron-donating components of milk and enable the quantitative determination of TAC in human milk and infant food. They are very important for routine and daily determinations of TAC in infant food, as well as for the control of milk freshness, especially after using fortifiers in neonatal units.", publisher = "MDPI", journal = "Proceedings", title = "Every Drop Counts - The Current Methods for Determining the Quality of Human Milk", volume = "91", number = "1", doi = "10.3390/proceedings2023091314" }
Lugonja, N., Stanković, D., Marinković, V., Avdalović, J., Spasić, S.,& Vrvić, M.. (2023). Every Drop Counts - The Current Methods for Determining the Quality of Human Milk. in Proceedings MDPI., 91(1). https://doi.org/10.3390/proceedings2023091314
Lugonja N, Stanković D, Marinković V, Avdalović J, Spasić S, Vrvić M. Every Drop Counts - The Current Methods for Determining the Quality of Human Milk. in Proceedings. 2023;91(1). doi:10.3390/proceedings2023091314 .
Lugonja, Nikoleta, Stanković, Dalibor, Marinković, Vesna, Avdalović, Jelena, Spasić, Snežana, Vrvić, Miroslav, "Every Drop Counts - The Current Methods for Determining the Quality of Human Milk" in Proceedings, 91, no. 1 (2023), https://doi.org/10.3390/proceedings2023091314 . .