Production of a novel opine dehydrogenase
2022
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Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Opine dehydrogenases are a family of NAD(P)H dependent oxidoreductases, whichcatalyze the reductive condensation of an α amino group from an amino acid with an αketo acid during anaerobic glycolysis by regenerating NAD. They are widespread incephalopods and mollusks. Opines are associated with crown gall tumor pathogenesiscaused by A. tumefaciens providing nutrients to the pathogen, and novel opine compoundsacting as metallophores have been identified. Besides, opine-type secondary aminedicarboxylic acids are chiral intermediates of angiotensin-converting enzyme inhibitors. Anovel enzyme originating from an extremophile bacterium, with assumed opinedehydrogenase function was successfully expressed in Escherichia coli STAR cells andpurified by affinity chromatography. Molecular mass determined by SDS-PAGE wasapproximately 40 kDa. The activity was measured by using pyruvate and alanine assubstrates, by which proved that it has opine dehydrogenase activity.
Кључне речи:
Opine dehydrogenasesИзвор:
Proceedings - Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry", September 22nd and 23rd, 2022, Novi Sad, Serbia, 2022, 79-79Издавач:
- Faculty of Chemistry
- Serbian Biochemical Society
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
Институција/група
IHTMTY - CONF AU - Kaličanin, Nevena AU - Balaž, Ana Marija AU - Prodanović, Olivera AU - Prodanović, Radivoje PY - 2022 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7099 AB - Opine dehydrogenases are a family of NAD(P)H dependent oxidoreductases, whichcatalyze the reductive condensation of an α amino group from an amino acid with an αketo acid during anaerobic glycolysis by regenerating NAD. They are widespread incephalopods and mollusks. Opines are associated with crown gall tumor pathogenesiscaused by A. tumefaciens providing nutrients to the pathogen, and novel opine compoundsacting as metallophores have been identified. Besides, opine-type secondary aminedicarboxylic acids are chiral intermediates of angiotensin-converting enzyme inhibitors. Anovel enzyme originating from an extremophile bacterium, with assumed opinedehydrogenase function was successfully expressed in Escherichia coli STAR cells andpurified by affinity chromatography. Molecular mass determined by SDS-PAGE wasapproximately 40 kDa. The activity was measured by using pyruvate and alanine assubstrates, by which proved that it has opine dehydrogenase activity. PB - Faculty of Chemistry PB - Serbian Biochemical Society C3 - Proceedings - Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry", September 22nd and 23rd, 2022, Novi Sad, Serbia T1 - Production of a novel opine dehydrogenase SP - 79 EP - 79 UR - https://hdl.handle.net/21.15107/rcub_cer_7099 ER -
@conference{ author = "Kaličanin, Nevena and Balaž, Ana Marija and Prodanović, Olivera and Prodanović, Radivoje", year = "2022", abstract = "Opine dehydrogenases are a family of NAD(P)H dependent oxidoreductases, whichcatalyze the reductive condensation of an α amino group from an amino acid with an αketo acid during anaerobic glycolysis by regenerating NAD. They are widespread incephalopods and mollusks. Opines are associated with crown gall tumor pathogenesiscaused by A. tumefaciens providing nutrients to the pathogen, and novel opine compoundsacting as metallophores have been identified. Besides, opine-type secondary aminedicarboxylic acids are chiral intermediates of angiotensin-converting enzyme inhibitors. Anovel enzyme originating from an extremophile bacterium, with assumed opinedehydrogenase function was successfully expressed in Escherichia coli STAR cells andpurified by affinity chromatography. Molecular mass determined by SDS-PAGE wasapproximately 40 kDa. The activity was measured by using pyruvate and alanine assubstrates, by which proved that it has opine dehydrogenase activity.", publisher = "Faculty of Chemistry, Serbian Biochemical Society", journal = "Proceedings - Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry", September 22nd and 23rd, 2022, Novi Sad, Serbia", title = "Production of a novel opine dehydrogenase", pages = "79-79", url = "https://hdl.handle.net/21.15107/rcub_cer_7099" }
Kaličanin, N., Balaž, A. M., Prodanović, O.,& Prodanović, R.. (2022). Production of a novel opine dehydrogenase. in Proceedings - Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry", September 22nd and 23rd, 2022, Novi Sad, Serbia Faculty of Chemistry., 79-79. https://hdl.handle.net/21.15107/rcub_cer_7099
Kaličanin N, Balaž AM, Prodanović O, Prodanović R. Production of a novel opine dehydrogenase. in Proceedings - Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry", September 22nd and 23rd, 2022, Novi Sad, Serbia. 2022;:79-79. https://hdl.handle.net/21.15107/rcub_cer_7099 .
Kaličanin, Nevena, Balaž, Ana Marija, Prodanović, Olivera, Prodanović, Radivoje, "Production of a novel opine dehydrogenase" in Proceedings - Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry", September 22nd and 23rd, 2022, Novi Sad, Serbia (2022):79-79, https://hdl.handle.net/21.15107/rcub_cer_7099 .