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Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects
dc.creator | Milenković, Mila | |
dc.creator | Ciasca, Gabriele | |
dc.creator | Bonasera, Aurelio | |
dc.creator | Scopelliti, Michelangelo | |
dc.creator | Marković, Olivera | |
dc.creator | Verbić, Tatjana | |
dc.creator | Todorović Marković, Biljana | |
dc.creator | Jovanović, Svetlana | |
dc.date.accessioned | 2023-12-19T14:25:38Z | |
dc.date.available | 2023-12-19T14:25:38Z | |
dc.date.issued | 2024 | |
dc.identifier.issn | 1011-1344 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/7093 | |
dc.description.abstract | The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L-cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure. | sr |
dc.language.iso | en | sr |
dc.publisher | Elsevier | sr |
dc.relation | info:eu-repo/grantAgreement/ScienceFundRS/Ideje/7741955/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200017/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS// | sr |
dc.relation | The Italian Ministry of University and Research (MURST, ex-MIUR) is acknowledged by A.B. for funding his research activities (PON “AIM: Attrazione e Mobilita ` Internazionale”, call AIM1809078-2, CUP B78D19000280001) | sr |
dc.rights | restrictedAccess | sr |
dc.source | Journal of Photochemistry and Photobiology, B: Biology | sr |
dc.subject | Graphene quantum dots | sr |
dc.subject | Antibacterial activity | sr |
dc.subject | Photodynamic therapy | sr |
dc.subject | Singlet oxygen production | sr |
dc.title | Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.citation.volume | 250 | |
dc.citation.spage | 112818 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 38041931 | |
dc.identifier.doi | 10.1016/j.jphotobiol.2023.112818 | |
dc.identifier.scopus | 2-s2.0-85179056140 | |
dc.type.version | publishedVersion | sr |