Cyclic voltammetry in diagnosis of ALS
2012
Аутори
Mudrinić, TihanaIgnjatović, Aleksandar
Mojović, Zorica
Jovanović, Dušan
Mojović, Miloš
Bačić, Goran
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The diagnosis of amyotrophic lateral sclerosis (ALS) based on ALS functional rating score (ALSFRS) is often shown to be unreliable and false. On the other hand, it is a fact that MRI studies of a brain of ALS patients showthe presence of iron deposits in precentral gyruses of gray matter (PGGM), meaning that blood-brain barrier is compromised. Therefore, various studies have been performed with the goal to detect excess of iron in the cerebrospinal fluid (CSF) of ALS patients (e.g. EPR detection of •OH radicals as products of Fenton reaction after supplementation of H2O2 to the CSF). Unfortunately, no acceptable correlation could emerge, probably caused by the presence of a range of iron complexes in CSF. Therefore, a different approach to detect iron states in CFS is required. The aim of thisworkwas to determine if there is a specific feature in CSF that distinguishes patients with ALS from those with purely motor peripheral neuropathy (PN) and healthy control subjects. CSF obtained f...rom ALS patients and normal controls were analyzed using the technique of cyclic voltammetry. The results show that, at potential of 1.1 - 1.2 V vs. Ag/AgCl electrode, for the ALS patients, the plateau appeared and the potential of oxygen evolutionwas shifted toward more positive values. These voltammogram features were not present for the control patients. The cyclic voltammetry is fast and inexpensive technique and showed to be promising candidate for evaluating new biomarkers for ALS.
Кључне речи:
amyotrophic lateral sclerosis / cerebrospinal fluid / cyclic voltammetry / biomarkersИзвор:
Book of Abstracts - Regional Biophysics Conference 2012, September 03-07, Kladovo-Belgrade, Serbia, 2012, 75-75Издавач:
- Biophysical Society of Serbia
Финансирање / пројекти:
- Наноструктурни функционални и композитни материјали у каталитичким и сорпционим процесима (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45001)
Институција/група
IHTMTY - CONF AU - Mudrinić, Tihana AU - Ignjatović, Aleksandar AU - Mojović, Zorica AU - Jovanović, Dušan AU - Mojović, Miloš AU - Bačić, Goran PY - 2012 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7016 AB - The diagnosis of amyotrophic lateral sclerosis (ALS) based on ALS functional rating score (ALSFRS) is often shown to be unreliable and false. On the other hand, it is a fact that MRI studies of a brain of ALS patients showthe presence of iron deposits in precentral gyruses of gray matter (PGGM), meaning that blood-brain barrier is compromised. Therefore, various studies have been performed with the goal to detect excess of iron in the cerebrospinal fluid (CSF) of ALS patients (e.g. EPR detection of •OH radicals as products of Fenton reaction after supplementation of H2O2 to the CSF). Unfortunately, no acceptable correlation could emerge, probably caused by the presence of a range of iron complexes in CSF. Therefore, a different approach to detect iron states in CFS is required. The aim of thisworkwas to determine if there is a specific feature in CSF that distinguishes patients with ALS from those with purely motor peripheral neuropathy (PN) and healthy control subjects. CSF obtained from ALS patients and normal controls were analyzed using the technique of cyclic voltammetry. The results show that, at potential of 1.1 - 1.2 V vs. Ag/AgCl electrode, for the ALS patients, the plateau appeared and the potential of oxygen evolutionwas shifted toward more positive values. These voltammogram features were not present for the control patients. The cyclic voltammetry is fast and inexpensive technique and showed to be promising candidate for evaluating new biomarkers for ALS. PB - Biophysical Society of Serbia C3 - Book of Abstracts - Regional Biophysics Conference 2012, September 03-07, Kladovo-Belgrade, Serbia T1 - Cyclic voltammetry in diagnosis of ALS SP - 75 EP - 75 UR - https://hdl.handle.net/21.15107/rcub_cer_7016 ER -
@conference{ author = "Mudrinić, Tihana and Ignjatović, Aleksandar and Mojović, Zorica and Jovanović, Dušan and Mojović, Miloš and Bačić, Goran", year = "2012", abstract = "The diagnosis of amyotrophic lateral sclerosis (ALS) based on ALS functional rating score (ALSFRS) is often shown to be unreliable and false. On the other hand, it is a fact that MRI studies of a brain of ALS patients showthe presence of iron deposits in precentral gyruses of gray matter (PGGM), meaning that blood-brain barrier is compromised. Therefore, various studies have been performed with the goal to detect excess of iron in the cerebrospinal fluid (CSF) of ALS patients (e.g. EPR detection of •OH radicals as products of Fenton reaction after supplementation of H2O2 to the CSF). Unfortunately, no acceptable correlation could emerge, probably caused by the presence of a range of iron complexes in CSF. Therefore, a different approach to detect iron states in CFS is required. The aim of thisworkwas to determine if there is a specific feature in CSF that distinguishes patients with ALS from those with purely motor peripheral neuropathy (PN) and healthy control subjects. CSF obtained from ALS patients and normal controls were analyzed using the technique of cyclic voltammetry. The results show that, at potential of 1.1 - 1.2 V vs. Ag/AgCl electrode, for the ALS patients, the plateau appeared and the potential of oxygen evolutionwas shifted toward more positive values. These voltammogram features were not present for the control patients. The cyclic voltammetry is fast and inexpensive technique and showed to be promising candidate for evaluating new biomarkers for ALS.", publisher = "Biophysical Society of Serbia", journal = "Book of Abstracts - Regional Biophysics Conference 2012, September 03-07, Kladovo-Belgrade, Serbia", title = "Cyclic voltammetry in diagnosis of ALS", pages = "75-75", url = "https://hdl.handle.net/21.15107/rcub_cer_7016" }
Mudrinić, T., Ignjatović, A., Mojović, Z., Jovanović, D., Mojović, M.,& Bačić, G.. (2012). Cyclic voltammetry in diagnosis of ALS. in Book of Abstracts - Regional Biophysics Conference 2012, September 03-07, Kladovo-Belgrade, Serbia Biophysical Society of Serbia., 75-75. https://hdl.handle.net/21.15107/rcub_cer_7016
Mudrinić T, Ignjatović A, Mojović Z, Jovanović D, Mojović M, Bačić G. Cyclic voltammetry in diagnosis of ALS. in Book of Abstracts - Regional Biophysics Conference 2012, September 03-07, Kladovo-Belgrade, Serbia. 2012;:75-75. https://hdl.handle.net/21.15107/rcub_cer_7016 .
Mudrinić, Tihana, Ignjatović, Aleksandar, Mojović, Zorica, Jovanović, Dušan, Mojović, Miloš, Bačić, Goran, "Cyclic voltammetry in diagnosis of ALS" in Book of Abstracts - Regional Biophysics Conference 2012, September 03-07, Kladovo-Belgrade, Serbia (2012):75-75, https://hdl.handle.net/21.15107/rcub_cer_7016 .