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dc.creatorMatić, Ivana Z.
dc.creatorZizak, Zeljko
dc.creatorSimonović, Mladen
dc.creatorSimonovic, Branislav
dc.creatorGođevac, Dejan
dc.creatorSavikin, Katarina
dc.creatorJuranić, Zorica
dc.date.accessioned2019-01-30T17:23:49Z
dc.date.available2019-01-30T17:23:49Z
dc.date.issued2010
dc.identifier.issn1096-620X
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/697
dc.description.abstractRed and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines-human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453 and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white wine polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential.en
dc.publisherMary Ann Liebert Inc, New Rochelle
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/145006/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Medicinal Food
dc.subjectcancer cellsen
dc.subjectcytotoxicityen
dc.subjectnormal peripheral blood mononuclear cellsen
dc.subjectred wine polyphenolsen
dc.subjectwhite wine polyphenolsen
dc.titleCytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cellsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСимоновиц, Младен; Матиц, Ивана; Савикин, Катарина; Симоновиц, Бранислав; Јураниц, Зорица; Зизак, Зељко; Гођевац, Дејан;
dc.citation.volume13
dc.citation.issue4
dc.citation.spage851
dc.citation.epage862
dc.citation.other13(4): 851-862
dc.citation.rankM22
dc.identifier.pmid20482276
dc.identifier.doi10.1089/jmf.2009.0193
dc.identifier.rcubConv_2535
dc.identifier.scopus2-s2.0-77955213675
dc.identifier.wos000280485000014
dc.type.versionpublishedVersion


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