The effects of nitric oxide and peroxynitrite on MnSOD (E. coli)
Само за регистроване кориснике
2005
Аутори
Filipović, MilošStanić, Dragana
Nikolić, Milan
Stojanović, Srđan
Raičević, Smiljana
Niketić, Vesna
Поглавље у монографији (Објављена верзија)
,
IOS Press
Метаподаци
Приказ свих података о документуАпстракт
In vitro studies demonstrated that peroxynitrite inactivates both human
recombinant MnSOD (hrMnSOD) and E. coli MnSOD causing enzyme tyrosine
residue(s) nitration. This led to a suggestion that human MnSOD nitration and
inactivation in vivo, detected in various deseases associated with oxidative stress and
overproduction of nitric monoxide (NO)–conditions that favor peroxynitrite
formation–are also caused by peroxynitrite. In a previous study we demonstrated
that the exposure of E. coli MnSOD to NO under the anaerobic conditions causes
NO conversion (dismutation) into reactive nitrosonium (NO
+
) and nitroxyl
(HNO/NO
–
) species, which produce enzyme modifications and inactivation (Niketic
et al., Free Rad. Biol. Med. 27: 992 (1999)). The present study shows that interaction
of NO with E. coli MnSOD leads to the formation of nitrating species capable
of nitrating and oxidizing enzyme tyrosine residues, as well as that these species
are less invasive than peroxynitrite i...n producing enzyme modifications and
inactivation. Low molecular mass thiols are shown to reduce enzyme inactivation
and NO-induced tyrosine nitration. The present study contributes to the understanding
of the nature of NO reaction with E. coli MnSOD and provides compelling
argument in support of the direct involvement of NO in MnSOD mediated
generation of nitrating species. Interaction of NO with MnSOD may represent a
novel mechanism by which MnSOD protects the cell from deleterious effects
associated with overproduction of NO. However, extensive MnSOD modifications
and inactivation associated with a prolonged exposure to NO will amplify toxic
effects caused by elevated cell superoxide and NO levels.
Кључне речи:
MnSOD / nitric oxideИзвор:
NATO Science Series, Series I: Life and Behavioural Sciences - Free Radicals and Diseases: Gene Expression, Cellular Metabolism and Pathophysiology, 2005, 367, 61-69Издавач:
- IOS Press
Финансирање / пројекти:
- Serbian Research Fund (Grant HE1569).
Институција/група
IHTMTY - CHAP AU - Filipović, Miloš AU - Stanić, Dragana AU - Nikolić, Milan AU - Stojanović, Srđan AU - Raičević, Smiljana AU - Niketić, Vesna PY - 2005 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6509 AB - In vitro studies demonstrated that peroxynitrite inactivates both human recombinant MnSOD (hrMnSOD) and E. coli MnSOD causing enzyme tyrosine residue(s) nitration. This led to a suggestion that human MnSOD nitration and inactivation in vivo, detected in various deseases associated with oxidative stress and overproduction of nitric monoxide (NO)–conditions that favor peroxynitrite formation–are also caused by peroxynitrite. In a previous study we demonstrated that the exposure of E. coli MnSOD to NO under the anaerobic conditions causes NO conversion (dismutation) into reactive nitrosonium (NO + ) and nitroxyl (HNO/NO – ) species, which produce enzyme modifications and inactivation (Niketic et al., Free Rad. Biol. Med. 27: 992 (1999)). The present study shows that interaction of NO with E. coli MnSOD leads to the formation of nitrating species capable of nitrating and oxidizing enzyme tyrosine residues, as well as that these species are less invasive than peroxynitrite in producing enzyme modifications and inactivation. Low molecular mass thiols are shown to reduce enzyme inactivation and NO-induced tyrosine nitration. The present study contributes to the understanding of the nature of NO reaction with E. coli MnSOD and provides compelling argument in support of the direct involvement of NO in MnSOD mediated generation of nitrating species. Interaction of NO with MnSOD may represent a novel mechanism by which MnSOD protects the cell from deleterious effects associated with overproduction of NO. However, extensive MnSOD modifications and inactivation associated with a prolonged exposure to NO will amplify toxic effects caused by elevated cell superoxide and NO levels. PB - IOS Press T2 - NATO Science Series, Series I: Life and Behavioural Sciences - Free Radicals and Diseases: Gene Expression, Cellular Metabolism and Pathophysiology T1 - The effects of nitric oxide and peroxynitrite on MnSOD (E. coli) VL - 367 SP - 61 EP - 69 UR - https://hdl.handle.net/21.15107/rcub_cer_6509 ER -
@inbook{ author = "Filipović, Miloš and Stanić, Dragana and Nikolić, Milan and Stojanović, Srđan and Raičević, Smiljana and Niketić, Vesna", year = "2005", abstract = "In vitro studies demonstrated that peroxynitrite inactivates both human recombinant MnSOD (hrMnSOD) and E. coli MnSOD causing enzyme tyrosine residue(s) nitration. This led to a suggestion that human MnSOD nitration and inactivation in vivo, detected in various deseases associated with oxidative stress and overproduction of nitric monoxide (NO)–conditions that favor peroxynitrite formation–are also caused by peroxynitrite. In a previous study we demonstrated that the exposure of E. coli MnSOD to NO under the anaerobic conditions causes NO conversion (dismutation) into reactive nitrosonium (NO + ) and nitroxyl (HNO/NO – ) species, which produce enzyme modifications and inactivation (Niketic et al., Free Rad. Biol. Med. 27: 992 (1999)). The present study shows that interaction of NO with E. coli MnSOD leads to the formation of nitrating species capable of nitrating and oxidizing enzyme tyrosine residues, as well as that these species are less invasive than peroxynitrite in producing enzyme modifications and inactivation. Low molecular mass thiols are shown to reduce enzyme inactivation and NO-induced tyrosine nitration. The present study contributes to the understanding of the nature of NO reaction with E. coli MnSOD and provides compelling argument in support of the direct involvement of NO in MnSOD mediated generation of nitrating species. Interaction of NO with MnSOD may represent a novel mechanism by which MnSOD protects the cell from deleterious effects associated with overproduction of NO. However, extensive MnSOD modifications and inactivation associated with a prolonged exposure to NO will amplify toxic effects caused by elevated cell superoxide and NO levels.", publisher = "IOS Press", journal = "NATO Science Series, Series I: Life and Behavioural Sciences - Free Radicals and Diseases: Gene Expression, Cellular Metabolism and Pathophysiology", booktitle = "The effects of nitric oxide and peroxynitrite on MnSOD (E. coli)", volume = "367", pages = "61-69", url = "https://hdl.handle.net/21.15107/rcub_cer_6509" }
Filipović, M., Stanić, D., Nikolić, M., Stojanović, S., Raičević, S.,& Niketić, V.. (2005). The effects of nitric oxide and peroxynitrite on MnSOD (E. coli). in NATO Science Series, Series I: Life and Behavioural Sciences - Free Radicals and Diseases: Gene Expression, Cellular Metabolism and Pathophysiology IOS Press., 367, 61-69. https://hdl.handle.net/21.15107/rcub_cer_6509
Filipović M, Stanić D, Nikolić M, Stojanović S, Raičević S, Niketić V. The effects of nitric oxide and peroxynitrite on MnSOD (E. coli). in NATO Science Series, Series I: Life and Behavioural Sciences - Free Radicals and Diseases: Gene Expression, Cellular Metabolism and Pathophysiology. 2005;367:61-69. https://hdl.handle.net/21.15107/rcub_cer_6509 .
Filipović, Miloš, Stanić, Dragana, Nikolić, Milan, Stojanović, Srđan, Raičević, Smiljana, Niketić, Vesna, "The effects of nitric oxide and peroxynitrite on MnSOD (E. coli)" in NATO Science Series, Series I: Life and Behavioural Sciences - Free Radicals and Diseases: Gene Expression, Cellular Metabolism and Pathophysiology, 367 (2005):61-69, https://hdl.handle.net/21.15107/rcub_cer_6509 .