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Karakterizacija tripsinima-sličnih enzima srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)

dc.creatorLončar, N.
dc.creatorBožić, Nataša
dc.creatorNenadović, Vera
dc.creatorIvanović, Jelisaveta
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:20:49Z
dc.date.available2019-01-30T17:20:49Z
dc.date.issued2009
dc.identifier.issn0354-4664
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/562
dc.description.abstractThe pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine.en
dc.description.abstractpH vrednost duž srednjeg creva larvi M. funereus se razlikuje, kisela je u regionu prednjeg dela, dok je u srednjem i zadnjem delu bazna. Elastazama i himotripsinima slična aktivnost je najveća u srednjem delu srednjeg creva dok je u prednjem delu detektovana mala vrednost, a u zadnjem delu zanemarljiva. Tripsinima slična aktivnost je detektovana duž celog srednjeg creva, s tim da se više od 90 % aktivnosti detektuje u prednjem delu srednjeg creva. Zastupljenost elastazama i himotripsinima sličnih endopeptidaza je zanemarljivo mala u poređenju sa zastupljenošću tripsinima sličnih enzima. U prednjem delu srednjeg creva nalaze se dve izoforme tripsinima sličnih enzima, sa baznim pI vrednostima, koje su skoro u potpunosti inhibirane benzamidinom.sr
dc.publisherUniversity of Belgrade, University of Novi Sad
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.rightsopenAccess
dc.sourceArchives of Biological Sciences
dc.subjectCerambycidaeen
dc.subjectMorimus funereusen
dc.subjecttrypsinen
dc.subjectmidgut peptidasesen
dc.subjectsynthetic substrateen
dc.subjectzymogramen
dc.titleCharacterization of trypsin-like enzymes from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvaeen
dc.titleKarakterizacija tripsinima-sličnih enzima srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)sr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractБожић, Наташа; Лончар, Н.; Ненадовић, Вера; Вујчић, З.; Ивановић, Јелисавета;
dc.citation.volume61
dc.citation.issue4
dc.citation.spage713
dc.citation.epage718
dc.citation.other61(4): 713-718
dc.citation.rankM23
dc.identifier.doi10.2298/ABS0904713L
dc.identifier.rcubConv_355
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/9199/560.pdf
dc.identifier.scopus2-s2.0-77949879656
dc.identifier.wos000273203500018
dc.type.versionpublishedVersion


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