Efficient enzyme@MOF composites for biocatalysis
Аутори
Stanišić, MarijaRistić, Predrag
Balaž, Ana Marija
Senćanski, Milan
Mitić, Dragana
Prodanović, Radivoje
Todorović, Tamara
Метаподаци
Приказ свих података о документуАпстракт
Novel industrial biocatalysts are needed which can offer advantages over traditional chemical processes with respect to sustainability, process efficiency, and reduced negative impact on the environment. Implementation of either native or mutated enzymes for various industrial applications is currently limited due to a lack of protein stability in harsh conditions. Metal-organic frameworks (MOFs), known for their ultra-high porosity and crystallinity, are perfect host materials that can protect guest enzymes from inhospitable external environments. Herein we show that the surface charge and chemistry of a protein determine its ability to seed MOF growth. We demonstrate that chemical modification of carbohydrate parts on the protein surface is an effective method for controlling biomimetic mineralization by zeolitic imidazolate framework-8 (ZIF-8). Protein charge, mixing of reactants, and stirring speed have been demonstrated to play important roles in controlling biomineralization reac...tion rate, particle shape, and morphology. This study highlights the important role played by protein surface chemistry in encapsulation and outlines a general method for facilitating the biomimetic mineralization of glycoproteins.
Кључне речи:
glycoproteins / biomimetic mineralisation / ZIF-8Извор:
Book of abstracts - EUROBIOTECH 8th Central European Congress of Life Sciences, 20-22 June 2022, Krakow, Poland, 2022, 138-138Финансирање / пројекти:
- SYMBIOSIS - Controllable Design of Efficient Enzyme"Mof Composites for Biocatalysis (RS-ScienceFundRS-Promis-6066997)
Напомена:
- Book of Abstracts
Институција/група
IHTMStanišić, M., Ristić, P., Balaž, A. M., Senćanski, M., Mitić, D., Prodanović, R.,& Todorović, T.. (2022). Efficient enzyme@MOF composites for biocatalysis. in Book of abstracts - EUROBIOTECH 8th Central European Congress of Life Sciences, 20-22 June 2022, Krakow, Poland, 138-138. https://hdl.handle.net/21.15107/rcub_cherry_5751
Stanišić M, Ristić P, Balaž AM, Senćanski M, Mitić D, Prodanović R, Todorović T. Efficient enzyme@MOF composites for biocatalysis. in Book of abstracts - EUROBIOTECH 8th Central European Congress of Life Sciences, 20-22 June 2022, Krakow, Poland. 2022;:138-138. https://hdl.handle.net/21.15107/rcub_cherry_5751 .
Stanišić, Marija, Ristić, Predrag, Balaž, Ana Marija, Senćanski, Milan, Mitić, Dragana, Prodanović, Radivoje, Todorović, Tamara, "Efficient enzyme@MOF composites for biocatalysis" in Book of abstracts - EUROBIOTECH 8th Central European Congress of Life Sciences, 20-22 June 2022, Krakow, Poland (2022):138-138, https://hdl.handle.net/21.15107/rcub_cherry_5751 .