Antioxidant properties of grape seed extract on human lymphocyte oxidative defence
Само за регистроване кориснике
2008
Аутори
Stanković, MiroslavaTešević, Vele
Vajs, Vlatka
Todorović, Nina
Milosavljević, Slobodan
Gođevac, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and C-13-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyt...e damage by ROS.
Кључне речи:
Vitis vinifera / Vitaceae / micronucleus test / antioxidant enzymes / human lymphocytes / LC/MS / NMRИзвор:
Planta Medica, 2008, 74, 7, 730-735Издавач:
- Georg Thieme Verlag Kg, Stuttgart
Финансирање / пројекти:
- Секундарни метаболити самониклих, лековитих биљака: изоловање, карактеризација и билошка активност (RS-MESTD-MPN2006-2010-142053)
DOI: 10.1055/s-2008-1074521
ISSN: 0032-0943
PubMed: 18496781
WoS: 000257284700005
Scopus: 2-s2.0-45849128011
Институција/група
IHTMTY - JOUR AU - Stanković, Miroslava AU - Tešević, Vele AU - Vajs, Vlatka AU - Todorović, Nina AU - Milosavljević, Slobodan AU - Gođevac, Dejan PY - 2008 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/391 AB - The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and C-13-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS. PB - Georg Thieme Verlag Kg, Stuttgart T2 - Planta Medica T1 - Antioxidant properties of grape seed extract on human lymphocyte oxidative defence VL - 74 IS - 7 SP - 730 EP - 735 DO - 10.1055/s-2008-1074521 ER -
@article{ author = "Stanković, Miroslava and Tešević, Vele and Vajs, Vlatka and Todorović, Nina and Milosavljević, Slobodan and Gođevac, Dejan", year = "2008", abstract = "The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and C-13-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS.", publisher = "Georg Thieme Verlag Kg, Stuttgart", journal = "Planta Medica", title = "Antioxidant properties of grape seed extract on human lymphocyte oxidative defence", volume = "74", number = "7", pages = "730-735", doi = "10.1055/s-2008-1074521" }
Stanković, M., Tešević, V., Vajs, V., Todorović, N., Milosavljević, S.,& Gođevac, D.. (2008). Antioxidant properties of grape seed extract on human lymphocyte oxidative defence. in Planta Medica Georg Thieme Verlag Kg, Stuttgart., 74(7), 730-735. https://doi.org/10.1055/s-2008-1074521
Stanković M, Tešević V, Vajs V, Todorović N, Milosavljević S, Gođevac D. Antioxidant properties of grape seed extract on human lymphocyte oxidative defence. in Planta Medica. 2008;74(7):730-735. doi:10.1055/s-2008-1074521 .
Stanković, Miroslava, Tešević, Vele, Vajs, Vlatka, Todorović, Nina, Milosavljević, Slobodan, Gođevac, Dejan, "Antioxidant properties of grape seed extract on human lymphocyte oxidative defence" in Planta Medica, 74, no. 7 (2008):730-735, https://doi.org/10.1055/s-2008-1074521 . .