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dc.creatorDojnov, Biljana
dc.creatorBožić, Nataša
dc.creatorBulajić, N.
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:16:40Z
dc.date.available2019-01-30T17:16:40Z
dc.date.issued2007
dc.identifier.issn0887-8013
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/364
dc.description.abstractImmunoblot analysis is not in wide use for diagnosis of invasive candidiasis, mostly because the procedure is not standardized and hence not reliable. This work describes a standardized method for C. albicans antigen extract preparation and immunochemical detection. The major improvement of the method is the preparation of combined antigen extract - consisting of the cell wall and cytosol antigens. The fungal cells and lysis buffer were mixed at a 1:3 ratio and disintegrated by ultrasound for six cycles of one minute each. After centrifugation, cytosol antigens were obtained in the supernatant and cell wall antigens were in the precipitate. Precipitate was dissolved in lysis buffer with 2% sodium dodecyl sulfate (SDS) and boiled at 100°C for 2 min. After centrifugation, the supernatant was combined with the previous one, so the extract of the combined antigens was obtained in the mixture. With those combined antigen extracts and with sera of three different groups of patients, immunoblot analysis showed sensitivity of 90.2%, specificity of 84.4%, and accuracy of 88.0%.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Clinical Laboratory Analysis
dc.subjectAntigenen
dc.subjectCandida albicansen
dc.subjectImmunobloten
dc.subjectInvasive candidiasisen
dc.titlePreparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysisen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВујчић, З.; Божић, Наташа; Булајић, Н.; Дојнов, Биљана;
dc.citation.volume21
dc.citation.issue6
dc.citation.spage406
dc.citation.epage412
dc.citation.other21(6): 406-412
dc.citation.rankM23
dc.identifier.pmid18022925
dc.identifier.doi10.1002/jcla.20206
dc.identifier.scopus2-s2.0-37049020502
dc.identifier.wos000251654300011
dc.type.versionpublishedVersion


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