Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis
Само за регистроване кориснике
2007
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Immunoblot analysis is not in wide use for diagnosis of invasive candidiasis, mostly because the procedure is not standardized and hence not reliable. This work describes a standardized method for C. albicans antigen extract preparation and immunochemical detection. The major improvement of the method is the preparation of combined antigen extract - consisting of the cell wall and cytosol antigens. The fungal cells and lysis buffer were mixed at a 1:3 ratio and disintegrated by ultrasound for six cycles of one minute each. After centrifugation, cytosol antigens were obtained in the supernatant and cell wall antigens were in the precipitate. Precipitate was dissolved in lysis buffer with 2% sodium dodecyl sulfate (SDS) and boiled at 100°C for 2 min. After centrifugation, the supernatant was combined with the previous one, so the extract of the combined antigens was obtained in the mixture. With those combined antigen extracts and with sera of three different groups of patients, immunobl...ot analysis showed sensitivity of 90.2%, specificity of 84.4%, and accuracy of 88.0%.
Кључне речи:
Antigen / Candida albicans / Immunoblot / Invasive candidiasisИзвор:
Journal of Clinical Laboratory Analysis, 2007, 21, 6, 406-412Финансирање / пројекти:
- Интеракције природних производа и њихових аналога са протеинима и нуклеинским киселинама (RS-MESTD-MPN2006-2010-142026)
DOI: 10.1002/jcla.20206
ISSN: 0887-8013
PubMed: 18022925
WoS: 000251654300011
Scopus: 2-s2.0-37049020502
Институција/група
IHTMTY - JOUR AU - Dojnov, Biljana AU - Božić, Nataša AU - Bulajić, N. AU - Vujčić, Zoran PY - 2007 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/364 AB - Immunoblot analysis is not in wide use for diagnosis of invasive candidiasis, mostly because the procedure is not standardized and hence not reliable. This work describes a standardized method for C. albicans antigen extract preparation and immunochemical detection. The major improvement of the method is the preparation of combined antigen extract - consisting of the cell wall and cytosol antigens. The fungal cells and lysis buffer were mixed at a 1:3 ratio and disintegrated by ultrasound for six cycles of one minute each. After centrifugation, cytosol antigens were obtained in the supernatant and cell wall antigens were in the precipitate. Precipitate was dissolved in lysis buffer with 2% sodium dodecyl sulfate (SDS) and boiled at 100°C for 2 min. After centrifugation, the supernatant was combined with the previous one, so the extract of the combined antigens was obtained in the mixture. With those combined antigen extracts and with sera of three different groups of patients, immunoblot analysis showed sensitivity of 90.2%, specificity of 84.4%, and accuracy of 88.0%. T2 - Journal of Clinical Laboratory Analysis T1 - Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis VL - 21 IS - 6 SP - 406 EP - 412 DO - 10.1002/jcla.20206 ER -
@article{ author = "Dojnov, Biljana and Božić, Nataša and Bulajić, N. and Vujčić, Zoran", year = "2007", abstract = "Immunoblot analysis is not in wide use for diagnosis of invasive candidiasis, mostly because the procedure is not standardized and hence not reliable. This work describes a standardized method for C. albicans antigen extract preparation and immunochemical detection. The major improvement of the method is the preparation of combined antigen extract - consisting of the cell wall and cytosol antigens. The fungal cells and lysis buffer were mixed at a 1:3 ratio and disintegrated by ultrasound for six cycles of one minute each. After centrifugation, cytosol antigens were obtained in the supernatant and cell wall antigens were in the precipitate. Precipitate was dissolved in lysis buffer with 2% sodium dodecyl sulfate (SDS) and boiled at 100°C for 2 min. After centrifugation, the supernatant was combined with the previous one, so the extract of the combined antigens was obtained in the mixture. With those combined antigen extracts and with sera of three different groups of patients, immunoblot analysis showed sensitivity of 90.2%, specificity of 84.4%, and accuracy of 88.0%.", journal = "Journal of Clinical Laboratory Analysis", title = "Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis", volume = "21", number = "6", pages = "406-412", doi = "10.1002/jcla.20206" }
Dojnov, B., Božić, N., Bulajić, N.,& Vujčić, Z.. (2007). Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis. in Journal of Clinical Laboratory Analysis, 21(6), 406-412. https://doi.org/10.1002/jcla.20206
Dojnov B, Božić N, Bulajić N, Vujčić Z. Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis. in Journal of Clinical Laboratory Analysis. 2007;21(6):406-412. doi:10.1002/jcla.20206 .
Dojnov, Biljana, Božić, Nataša, Bulajić, N., Vujčić, Zoran, "Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis" in Journal of Clinical Laboratory Analysis, 21, no. 6 (2007):406-412, https://doi.org/10.1002/jcla.20206 . .